Navigation Links
UW-Madison engineers squeeze secrets from proteins

Proteins, one of the basic components of living things, are among the most studied molecules in biochemistry. Understanding how proteins form or "fold" from sequenced strings of amino acids has long been one of the grand challenges of biology.

A common belief holds that the more proteins are confined by their environment, the more stable - or less likely to unfold - they become. Now, as reported on the cover of the March issue of Biophysical Journal, a team of chemical and biological engineers from UW-Madison shows that premise to be untrue. While confinement plays an important role, other factors are also at play.

"Most research in this area looked at proteins in free solution when in fact, most proteins are confined in some way," says Juan de Pablo, a chemical and biological engineer at the University of Wisconsin-Madison. "What we demonstrate for the first time is that the stability of proteins under severe confinement, which is really the relevant way of looking at them for numerous applications, depends on their shape, their size and their interactions with the environment. It is a delicate balance between the energy available to fold the protein and entropy, or it's desire to be in the unfolded state."

De Pablo's research team developed a method to precisely calculate the entropy and determine how much of a protein's stability change upon confinement to attribute to energy and how much to entropy. "This is the important part of the calculation," de Pablo adds.

Protein stability is an incredibly important property in myriad applications, de Pablo says. Consider laundry detergent. A popular ad for detergent once claimed that "protein gets out protein." The idea behind this is that engineered enzymes are at work in the wash breaking down elements of a stain.

"Once a protein is folded, you can actually unfold it or destabilize it, either by heating it up, or by adding solvents to the system, like urea for example, that just destroy the folded structure of the protein. How resilient the protein is to these assaults is what we often call stability," de Pablo says. "Detergents like the ones you use to wash your clothes have enzymes that break the fat in stains. When you put you clothes in hot water in your washing machine, you want your detergents to withstand those high temperatures. So what people do is engineer enzymes that do not unfold when you put them in hot water. They design enzymes that are more stable than normal enzymes at high temperatures."

To better understand protein folding, de Pablo's team built computer models of proteins under different types of confinement. These models were then simulated to gain a better understand of protein stability. Working with the UW-Madison Nanoscale Science and Engineering Center (NSEC), funded by the National Science Foundation, the researchers will continue to refine their models with the goal of confining, folding and measuring the stability of proteins under more realistic conditions.


'"/>

Source:University of Wisconsin-Madison


Related biology news :

1. UW-Madison scientists zero in on drugs sweet spots
2. Duke engineers develop new 3-D cardiac imaging probe
3. MIT engineers an anti-cancer smart bomb
4. Scientists and engineers apply natures design to human problems
5. Bioengineers create stable networks of blood vessels
6. Rice bioengineers pioneer techniques for knee repair
7. MIT engineers probe spiders polymer art
8. Boston University biomedical engineers win major grant for pursuit of the $1,000 Genome
9. UWs Rosetta software to unlock secrets of many human proteins
10. Scientists reveal molecular secrets of the malaria parasite
11. Marine sponge yields nanoscale secrets
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/16/2017)... TEANECK, N.J. , May 16, 2017  Veratad ... leading provider of online age and identity verification solutions, ... the K(NO)W Identity Conference 2017, May 15 thru May ... Ronald Regan Building and International Trade Center. ... across the globe and in today,s quickly evolving digital ...
(Date:4/24/2017)... , April 24, 2017 ... and partner with  Identity Strategy Partners, LLP (IdSP) ... "With or without President Trump,s March 6, 2017 ... Terrorist Entry , refugee vetting can be instilled with ... resettlement. (Right now, all refugee applications are suspended ...
(Date:4/17/2017)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... its 2016 Annual Report on Form 10-K on Thursday April 13, ... ... the Investor Relations section of the Company,s website at http://www.nxt-id.com ... http://www.sec.gov . 2016 Year Highlights: ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... NJ (PRWEB) , ... October 11, 2017 , ... Personal eye wash is a basic ... one eye at a time. So which eye do you rinse first if a dangerous ... have Plum Duo Eye Wash with its unique dual eye piece. , “Whether ...
(Date:10/11/2017)... , ... October 11, 2017 , ... Disappearing forests and ... lives of over 5.5 million people each year. Especially those living in larger cities ... Treepex - based in one of the most pollution-affected countries globally - decided to ...
(Date:10/10/2017)... , ... October 10, 2017 , ... ... Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of the ... for Excellence in Volunteer Experience from US2020. , US2020’s mission is to change ...
(Date:10/10/2017)... ... October 10, 2017 , ... The ... prestigious awards honoring scientists who have made outstanding contributions to analytical ... during Pittcon 2018, the world’s leading conference and exposition for laboratory science, which ...
Breaking Biology Technology: