Navigation Links
UF Researchers Map Bacterial Proteins That Cause Tooth Loss

and in most cases it’s proteins that are vaccine and drug targets.?/p>

The scientists have been trying to understand how P. gingivalis interacts with healthy oral tissues to cause such devastation. In this study, they used cutting-edge molecular research techniques to map all the proteins ?known as the proteome ?produced by P. gingivalis. Ultimately, the researchers were able to fill hundreds of gaps in the organism’s sequence of roughly 2,000 proteins.

“The approach used in this study is very exciting,?said Hansel Fletcher, Ph.D., an associate professor of microbiology and molecular genetics at Loma Linda University in Loma Linda, Calif. “For the first time, we are able to see that the more than 200 so-called ‘hypothetical?proteins in P. gingivalis are expressed and have specific functions.?/p>

Until now, scientists had identified less than 2 percent of the pathogen’s proteins and had to guess at what other proteins might be present in the proteome based on similarities to other known proteins, said Fletcher.

“This study has done two things to advance that,?Lamont said. “We’ve identified the complete protein complement of the organism, and we’ve looked at how those proteins are expressed when the organism is in an environment that closely mimics an oral situation.?/p>

To do this, Lamont and his colleagues compared the proteins secreted by P. gingivalis when grown in a medium containing human gum cell proteins with the proteins produced by the bacteria when grown in a neutral medium. Bacterial proteins from the two conditions were separated using a new technique called Multidimensional Protein Identification Technology, or MudPIT.

Once separated, mass spectrometry was used to measure each protein’s mass and charge, identifiers as unique to proteins as the whorls of fingerprints are to people.

The spectrometry measurements were fed into a computer database to create a computational model of the P. gingivalis proteome, resul
'"/>

Source:University Of Florida Health Science Center


Page: 1 2 3

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
11. Researchers develop rapid diagnostic tool for pathogen identification
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Researchers Map Bacterial Proteins That Cause Tooth Loss

(Date:10/14/2014)... of researchers, led by the Chinese Academy of Agricultural ... Genetics a brief genomic history of tomato breeding, ... plant. , The C.M. Rick Tomato Genetics Resource Center ... study by providing seed of both cultivated tomato varieties ... on the first tomato genome sequence completed just two ...
(Date:10/14/2014)... Over the past decades, we have seen numerous tragic ... needs of infants for social contact and stimulation has ... , Infancy and childhood are critical life ... generation of research suggests that enriched environments, full of ... function. In contrast, deprivation and stress may compromise cortical ...
(Date:10/14/2014)... oral biologic medication has successfully treated chronic, precancerous inflammation ... study authored by an MD/PhD student in the University ... , The study is featured on the cover of ... was published online ahead of print in September. The ... Inflammatory cells in the colon, or polyps, are very ...
Breaking Biology News(10 mins):Institutional rearing may increase risk attention-deficit disorder 2Oral drug reduces formation of precancerous polyps in the colon, UB researchers find 2Oral drug reduces formation of precancerous polyps in the colon, UB researchers find 3
... When a foreign object such as a catheter enters the ... into a slick coating a biofilm that is ... cannot be defeated by a basic approach of conventional means. ... reveals the key players in the network and how they ...
... 2010 China PharmaHub Corp. (OTC Bulletin Board: ... agreement to be a distributor for Integrated Biometrics ("IB"), ...  PharmaHub will have the exclusive distribution rights in China, ... to sell IB products to customers in the Pharmaceutical, ...
... soldiers in the battlefield healthy, the U.S. Army is exploring ... Current techniques for analyzing water in the field can take ... Lipkens of Western New England College in Springfield, Massachusetts and ... They are working on an alternative technology that uses sound ...
Cached Biology News:Research provides new leads in the case against drug-resistant biofilms 2China PharmaHub Signs Distribution Agreement with Integrated Biometrics 2China PharmaHub Signs Distribution Agreement with Integrated Biometrics 3
(Date:10/20/2014)... Oct. 20, 2014 Mapp Biopharmaceutical,s valiant ... antibody therapeutic to fight the Ebola outbreak will ... time-consuming the production of pharmaceuticals can be, according ... said that while some may be taken aback ... those with industry knowledge are well aware of ...
(Date:10/20/2014)... /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today ... to $5.8 million and provides a good start to Q4.  The ... America and one in the Middle East ... record levels," said Peter Bruijns , President & CEO. "Total ... Q3 than they have been for any complete year since the ...
(Date:10/20/2014)... Oct. 20, 2014 PureTech , a ... big healthcare problems, announced today the closing of ... participation from Invesco Perpetual, a $120 Billion group ... drive PureTech,s existing pipeline forward and to advance ... the scientific creativity to really go for the ...
(Date:10/20/2014)... 20, 2014 Local veterinary surgeon, Dr. ... investigational study of donor stem cells for dogs with ... therapy and has performed clinical stem cell therapy for ... to determine if a single injection of donor stem ... help reduce pain and inflammation in the treated joints. ...
Breaking Biology Technology:Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 2Kalorama: ZMapp Highlights Need For Faster Biopharmaceutical Production 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... Approve Merger With Integra on October 11, ... ... September 5 IsoTis, Inc.,(NASDAQ: ISOT ) ("IsoTis"), an orthobiologics ... U.S. Securities and,Exchange Commission ("SEC") in connection with the IsoTis-Integra,LifeSciences Holdings ...
... WEST BRIDGEWATER, Mass., Sept. 5 Pressure,BioSciences, Inc. ... has received,approval to CE Mark the Barocycler NEP2320, ... (PCT) instrument. The,Company also announced that it has ... indicating that the Barocycler NEP2320 has been found ...
... 5 Thermo Fisher,Scientific Inc. (NYSE: TMO ... the instrument sales business of Davis Inotek Instruments, ... and excludes its,related calibration services. Davis Instruments is ... instruments, serving customers in a,wide range of industries ...
Cached Biology Technology:IsoTis Files Definitive Proxy Statement 2IsoTis Files Definitive Proxy Statement 3IsoTis Files Definitive Proxy Statement 4Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 2Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 3Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 4Thermo Fisher Scientific Acquires Instrument Sales Business of Davis Inotek 2Thermo Fisher Scientific Acquires Instrument Sales Business of Davis Inotek 3Thermo Fisher Scientific Acquires Instrument Sales Business of Davis Inotek 4
... Sytem is a highly advanced digital ... digital imaging and automation technology that ... analysis, archving/retrieval and colored professional reporting. ... industry's fastest scanning and detection modules ...
... overlay agarose is used for second-dimension ... strips in place in PROTEAN Plus ... incorporated to allow monitoring of electrophoresis ... 0.75% agarose in 1x Tris, glycine, ...
... ReadyPrep overlay agarose, is a low ... sample setup to secure IPG strips in ... dye is incorporated to allow monitoring of ... 0.5% low melting point agarose in 1x ...
... super cooling coil, Trans-Blot cell, ... enzyme, high-intensity, and extended electrophoretic ... the Trans-Blot cell. Use of ... connection to an external water ...
Biology Products: