Navigation Links
UCR researchers design chip that can improve citrus varieties

UC Riverside researchers, in partnership with Affymetrix, Inc., have designed a chip ?the GeneChip® Citrus Genome Array ?that can improve citrus varieties and suggest ways to better manage them. By helping determine which genes are turned on in a tissue of citrus ?genes that are associated with taste, acidic content and disease, for example ?the chip provides information useful to researchers for rectifying existing problems and making improvements to the fruit.

The citrus array will be used to develop new diagnostic tools for the improvement of citrus agriculture and post-harvest fruit handling, as well as to understand mechanisms underlying citrus diseases. Researchers will study traits pertinent to the citrus industry such as easy peeling, seedlessness, flavor components, pest and disease control, nutritional characteristics, and reproductive development.

"The citrus array helps us quickly examine a certain trait in citrus," said Mikeal Roose, a professor of genetics in the Department of Botany and Plant Sciences at UCR and a leader of the three-year research project. "For a trait posing a problem for the consumer, such as an undesirable flavor, we can identify genes associated with the trait and target these for correction to improve the flavor. The chip also helps us address citrus diseases by helping us see what happens in cells when a citrus plant is under attack from a virus. And with this chip we can better understand what happens at the cellular level when oranges are put in cold storage after they are harvested, leading eventually to better methods of storage that improve fruit flavor."

Manufactured by Affymetrix, Inc., the GeneChip® Citrus Genome Array is made up of a glass wafer on to which nearly one million different pieces of citrus DNA are deposited on a grid or microarray using methods similar to those used to produce computer chips. The glass wafer is encased in a plas tic container somewhat smaller than the size of a credit card.

To use the chip, researchers purify total RNA (which reflects the genes expressed in the tissue) from plant tissue, make a copy of these molecules with a chemical tag added, and then "wash" the chip with the RNA sample. If a gene is being expressed in the tissue, its corresponding RNA will be present and bind to the complementary DNA sequences on the chip. The locations of the bound RNA have a visible signal because of the tag, rather like bright and dim pixels on a computer screen. Analysis of which pieces of DNA on the chip have signals indicates which genes are expressed in the tissue.

The chip is the first commercial citrus microarray and allows analysis of expression of more than 20,000 different genes. The array will also be used to develop a detailed genetic map of citrus that will help researchers locate many genes. The map location information will be used to make the development of new varieties more efficient.

"This industry-supported effort both added to and made use of publicly available citrus sequences to develop an entirely new tool that will benefit all citrus researchers and help sustain the citrus industry locally and worldwide," said Timothy Close, a professor of genetics at UCR and a co-leader of the project. "We owe a special thanks to colleagues in the citrus community: Abhaya Dandekar at UC Davis, Bob Shatters, Jose Chaparro and Greg McCollum at the USDA Horticultural Research Lab, and Avi Sadka at Volcani Institute in Israel for sharing the full content of their citrus sequence data.

"Other colleagues in the United States, Japan and Spain who deposited sequences to the public repository maintained by the National Center for Biotechnology Information also made valuable contributions. The use of all available public data resulted in very nice coverage of the citrus genome. We are pleased with the outcome ?the initial data from the citrus GeneChip have fulfilled our highest expectations."


'"/>

Source:University of California - Riverside


Related biology news :

1. NYU researchers simulate molecular biological clock
2. Vital step in cellular migration described by UCSD medical researchers
3. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
4. UCSD researchers maintain stem cells without contaminated animal feeder layers
5. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
6. New protein discovered by Hebrew University researchers
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Agilent Technologies releases automated literature search tool for biology researchers
9. Self-assembled nano-sized probes allow Penn researchers to see tumors through flesh and skin
10. Yale researchers identify molecule for detecting parasitic infection in humans
11. US life expectancy about to decline, researchers say
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/14/2017)... Feb. 14, 2017  Wake Forest Baptist Medical Center ... new chief executive officer (CEO). Freischlag joins the medical ... D. McConnell , M.D., who last year announced that ... Medical Center, after leading it since 2008.   ... of Wake Forest Baptist,s academic health system, which includes ...
(Date:2/13/2017)... Feb. 13, 2017 Former 9/11 Commission border ... Committee, Janice Kephart of Identity Strategy Partners, ... Donald Trump,s "Executive Order: Protecting the Nation From ... 2017):  "As President Trump,s ,Travel Ban, Executive ... now essentially banned the travel ban, it is important ...
(Date:2/9/2017)... The biomass boiler market report by Transparency Market Research ... in terms of revenue (US$ Mn) based on the ... boilers has been segmented on the basis of feedstock ... based on feedstock type, has been segmented into woody ... urban residues, and others. On the basis of product ...
Breaking Biology News(10 mins):
(Date:2/27/2017)... , February 27, 2017 ... Stock-Callers.com for evaluation today, and they are: Anthera Pharmaceuticals ... Biosciences of California Inc. (NASDAQ: PACB), and Conatus Pharmaceuticals ... article on ETF Trends, market observers are growing more ... administration could enact reforms to free cash held overseas ...
(Date:2/26/2017)... (PRWEB) , ... February 26, 2017 , ... Rob Lowe ... public television series. This series, called "Informed," focuses on issues that are important to ... on the climate change issue, which has been a hot topic around the world ...
(Date:2/24/2017)... Tenn. , Feb. 24, 2017 ... ("Provectus" or the "Company"), a clinical-stage oncology and ... regarding the deadline to participate in its previously ... units, consisting of shares of common stock and ... and holders of listed warrants. ...
(Date:2/24/2017)... Island, NY (PRWEB) , ... February 24, 2017 , ... ... rate. Founded in late 2014, FireflySci had the goal of bringing their powerful ... that goal continues to shape the path that FireflySci is going on as they ...
Breaking Biology Technology: