Navigation Links
UCLA scientists reveal how Nipah virus infects cells

UCLA scientists have discovered how the deadly Nipah virus infiltrates human cells to cause encephalitis. Designated as a potential bioterrorism agent by the National Biodefense Research Agenda, the virus exploits a protein essential to embryonic development to enter cells and launch its attack. The online edition of Nature reports the findings July 6.

"In its natural state, the Nipah virus can be used as a potential bioterrorism agent capable of devastating an entire country's public health and economy," said Dr. Benhur Lee, principal investigator and UCLA assistant professor of microbiology, immunology and molecular genetics. "Now that we understand how the virus operates, we can develop vaccines and drugs to block Nipah from entering the cells. This will help prevent infection and halt outbreaks before they reach epidemic proportions."

Since 1998, the Nipah virus has triggered disease outbreaks in Australia, Singapore, Malaysia and Bangladesh. Animals spread the virus to people, where it causes life-threatening respiratory and neurological diseases that kill up to 70 percent of patients ?a danger level equivalent to the Ebola virus.

To infect a cell, viruses must bind to a viral-specific receptor on the cell's surface in order to penetrate it. Lee's team identified a cell receptor called Ephrin-B2 as the key used by the Nipah virus to unlock the cells.

Located on brain cells and cells lining the blood vessels, Ephrin-B2 is critical to nervous system development and the growth of blood vessels in human and animal embryos. Ephrin-B2 is found in humans, horses, pigs and bats, which may explain why the infection can jump so easily from one species to another.

Collaborating with the University of Pennsylvania, the UCLA team applied tools of advanced molecular biology as well as old-fashioned detective work to track down the identity of the Ephrin-B2 receptor.

The researchers created a bait: they stitched the Nipah protein to part of a human antibody, like a worm on a fishing hook. When they placed this bait on cells at risk for Nipah infection, the antibody attached to a receptor on the cell surface. When placed on Nipah-resistant cells, however, the bait did not bind to the cell.

The scientists used an instrument that sorts molecules by weight to identify Ephrin-B2 as the receptor that bound to the bait.

To confirm their findings, the UCLA team engineered a harmless virus with Nipah virus proteins embedded in its coat. The decoy virus successfully infected cells vulnerable to the Nipah virus, but could not infect Nipah-resistant cells.

In the final step, the decoy virus entered nerve cells and cells lining blood vessels by latching onto Ephrin-B2, proving that the receptor is the same doorway that the real Nipah virus enters to infect these cells.

"We now can screen for small molecules that will block viral entry via Ephrin B-2 and develop them as therapeutic drugs," said Lee. "Because pigs are particularly susceptible to Nipah infection, public health officials could use these drugs to protect the animals, pig farmers and first-line responders, like paramedics, from a Nipah virus outbreak."

In the United States, agricultural experts estimate the value of pigs alone in the hog-farming industry at $8.6 billion.

The first reported outbreak of Nipah virus in Malaysia occurred between 1998 and 1999, sickening 265 people and killing 105. Spread from bats to pigs to humans, the outbreak infected more than 200 pig farmers and killed 40 percent. Desperate to contain the outbreak, the government ordered its military to kill more than 1 million pigs, resulting in economic devastation to the country.

In Bangladesh, death rates of repeated outbreaks of Nipah virus in the past four years have risen to 70 percent, suggesting that the virus is growing more lethal.


Source:University of California - Los Angeles

Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
3. UAB scientists discover the origin of a mysterious physical force
4. Fox Chase Cancer Center scientists identify immune-system mutation
5. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
6. U-M scientists find genes that control growth of common skin cancer
7. UCLA scientists transform HIV into cancer-seeking missile
8. RNA project to create language for scientists worldwide
9. Carnegie Mellon scientists develop tool that uses MRI to visualize gene expression in living animals
10. To control germs, scientists deploy tiny agents provocateurs
11. Leprosy microbes lead scientists to immune discovery
Post Your Comments:

(Date:11/4/2015)... , November 4, 2015 ... new market report published by Transparency Market Research "Home Security ... Trends and Forecast 2015 - 2022", the global home security ... 30.3 bn by 2022. The market is estimated to ... period from 2015 to 2022. Rising security needs among ...
(Date:10/29/2015)... ARBOR, Mich. , Oct. 29, 2015 /PRNewswire/ ... Eurofins Genomics for U.S. distribution of its DNA ... DNA-seq kit and Rubicon,s new ThruPLEX Plasma-seq kit. ... to enable the preparation of NGS libraries for ... plasma for diagnostic and prognostic applications in cancer ...
(Date:10/27/2015)... 2015 Munich, Germany ... technology (ASGM) automatically maps data from mobile eye tracking ... , so that they can be quantitatively analyzed with ... Munich, Germany , October 28-29, 2015. SMI,s Automated ... mobile eye tracking videos created with SMI,s Eye ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... CITY , Nov. 24, 2015 /PRNewswire/ - ... "Company") announced today that the remaining 11,000 post-share ... Share Purchase Warrants (the "Series B Warrants") subject ... were exercised on November 23, 2015, which will ... Shares.  After giving effect to the issuance of ...
(Date:11/24/2015)... ... November 24, 2015 , ... In harsh ... Insertion points for in-line sensors can represent a weak spot where leaking process ... series of retractable sensor housings , which are designed to tolerate extreme process ...
(Date:11/24/2015)... ... November 24, 2015 , ... ... Technologies, Inc., on being named to Deloitte's 2015 Technology Fast 500 list of ... OrthoAccel manufactures AcceleDent®, a FDA-cleared, Class II medical device that speeds up orthodontic ...
(Date:11/24/2015)... 2015 Capricor Therapeutics, Inc. (NASDAQ: ... discovery, development and commercialization of first-in-class therapeutics, today announced ... is scheduled to present at the 2015 Piper Jaffray ... EST, at The Lotte New York Palace Hotel in ... . --> . ...
Breaking Biology Technology: