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UCLA researchers unravel a mystery about DNA

del implies that the scrunched DNA is expelled from the enzyme channel at predictable sites that are available for interaction with transcription regulatory proteins. Beyond resolving the mechanism for initiation, the significance of this work is in pointing out an important regulation "checkpoint." Scrunched DNA is likely to play a major role in future studies of transcription regulation, and possibly become a focus for antibiotic drug discovery efforts.

"These are issues that we were not able to resolve until the development of the single molecule methods that we employed in these studies," Ebright said. "These methods involve detecting and manipulating single molecules, one at a time -- a breakthrough in its own right."

"The study of molecular machines, the dynamics of their moving parts and their translocation on molecular tracks is of great interest to nanotechnologists at the CNSI," said Weiss, the leader of the UCLA team. "Beyond furthering the understanding of transcription regulation, the novel methods and findings of this work will aid future studies of other molecular machines involved in cell replication, transcription and protein synthesis."


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Source:University of California - Los Angeles


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