Navigation Links
U-M scientists discover identifying markers for primitive blood-forming stem cells

s gives you similar or better purity than 10 to 12 of the best classical markers combined. It reduces the skill and time required to pick out a hematopoietic stem cell and makes the process simpler and less labor intensive."

"Many of the current markers we use to purify HSCs are expressed as a gradient from high to low across the hematopoietic cell hierarchy," says Omer H. Yilmaz, co-first author and a student in the Medical School's M.D./Ph.D. program. "What's really exciting about this finding is that now we have markers that turn on and off abruptly as stem cells differentiate."

Kiel and Yilmaz used microarray analysis to measure levels of gene activity in three types of cells ?hematopoietic stem cells, multipotent progenitor cells and fully developed bone marrow cells. They selected genes that were expressed at higher levels in the HSC population, as opposed to the other two types of cells. CD150, the founding member of the SLAM family of cell surface receptors, was near the top of the list.

To see if CD150 was expressed on hematopoietic stem cells, Kiel and Yilmaz transplanted CD150+ and CD150- cells into laboratory mice whose bone marrow had been destroyed by lethal doses of radiation. Healthy bone marrow was restored in six of six mice receiving CD150+ cells, but reconstitution was successful in only one of nine mice receiving CD150- cells.

When they followed the same procedure to test another SLAM marker, CD244, the results were completely opposite. Cells with CD244 receptors on their surface membranes were unable to reconstitute bone marrow in irradiated mice for more than a few weeks, while cells without CD244 receptors were able to reconstitute indefinitely. This indicated that CD244+ cells were a marker for multipotent progenitor cells (MPPs), but not for hematopoietic stem cells.

In a third step, the team tested another SLAM marker called CD48, and found it to be expressed only on more advanced cells. It was not p
'"/>

Source:University of Michigan Health System


Page: 1 2 3 4

Related biology news :

1. Wisconsin scientists grow critical nerve cells
2. UCSB scientists probe sea floor venting to gain understanding of early life on Earth
3. UAB scientists discover the origin of a mysterious physical force
4. Fox Chase Cancer Center scientists identify immune-system mutation
5. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
6. U-M scientists find genes that control growth of common skin cancer
7. UCLA scientists transform HIV into cancer-seeking missile
8. RNA project to create language for scientists worldwide
9. Carnegie Mellon scientists develop tool that uses MRI to visualize gene expression in living animals
10. To control germs, scientists deploy tiny agents provocateurs
11. Leprosy microbes lead scientists to immune discovery
Post Your Comments:
(Date:10/10/2008)..., MARSHFIELD, MILWAUKEE, MADISON Governor Jim D...een four Wisconsin research institutions to advanc...that proactively addresses diseases. The Wisconsin...ffort among the Marshfield Clinic, Medical College...l of Medicine and Public Health (UWSMPH) and UW-Mi...
(Date:10/10/2008)... New Haven, Conn. Yale scientists have created n... electronics that are both sensitive and specific ...detection, according to a report in Nano Letters ...y highly specific antigens signatures of bacteria... cells are activated, they produce acid, and gener...
(Date:10/9/2008)... Convenient online registration to the IOF World ...ed rates, is available only until October 31, 2008...ration, please visit http://www.iofbonehealth.org...es are available for participants from non-OECD co...8 only on-site registration, at higher rates, will...
(Date:10/9/2008)... , Will climate change exceed life,s ability to r...in the Oct. 10, 2008 issue of the journal, Scienc...ostered by the Smithsonian Tropical Research Insti... , As an extremely diverse region of rainforest a...ose as a result of global warming. Some disagree, ...
Breaking Biology News(10 mins):Governor Doyle announces historic genomic research collaboration 2Sensitive nanowire disease detectors made by Yale scientists 2Smithsonian perspective: Biodiversity in a warmer world 2Dartmouth researchers advance cellulosic ethanol production 7558 1Dartmouth researchers advance cellulosic ethanol production 7558 2Carbon molecule with a charge could be tomorrows semiconductor 7557 1Carbon molecule with a charge could be tomorrows semiconductor 7557 2Mylan to Retain Dey L P Its Specialty Pharmaceutical Subsidiary 26164 1Mylan to Retain Dey L P Its Specialty Pharmaceutical Subsidiary 26164 2Mylan to Retain Dey L P Its Specialty Pharmaceutical Subsidiary 26164 3Mylan to Retain Dey L P Its Specialty Pharmaceutical Subsidiary 26164 4Encouraging Gene Therapy Results in Genetic Form of Blindness 2990 1Encouraging Gene Therapy Results in Genetic Form of Blindness 2990 2Encouraging Gene Therapy Results in Genetic Form of Blindness 2990 3
...ditions early in the year mean that 2006 is shapin...s in almost four decades, according to Art Shapiro..., That,s a turnaround from last spring, when milli...he Central Valley. But other species have seen ste...om the region altogether. , "It has been the wors...
...ies that mimic the way insects, plants and animals...ur dependence on energy, according to new research... , When faced with engineering difficulties, such ... up to 70 per cent of man-made technologies manipu...order to resolve the problem. , However, new rese...
...ucted by Delft University of Technology has brough...friendly fuel bio-ethanol a great deal closer to f... was an important factor in this. His research in ...f certain sugars from agricultural waste to ethano...ee for his research into the subject. , The searc...
...lth & Science University study is adding crede...is what bonds stem cells with bone marrow cells to... School of Medicine found that transplanted cells ...tinal stem cells of both normal and diseased tissu...ls, known as the epithelium. The findings, reporte...
Other Biology News:Where have all the butterflies gone? 2Copying nature could save us energy, study shows 2Producing bio-ethanol from agricultural waste a step closer 2OHSU study says stem cell 'fusion' occurs in tumors 2OHSU study says stem cell 'fusion' occurs in tumors 3
(Date:10/10/2008)...ss., Oct. 10 /- Boston Scie...t has received approval from the,U.S. Food and Dru...tion,TAXUS(R) Liberte(R) Paclitaxel-Eluting Corona...AXUS Liberte stent early next month in the United,...f its TAXUS(R),Expresss2(TM) Atom(TM) Paclitaxel-E...
(Date:10/10/2008)...iangsu, CHINA, Oct. 10 /Xinhua-- Beik...ch.com ) a global leader in,stem cell research an...nced,today it has signed eight separate mutual coo...ld,s most outstanding stem cell research organizat...tablished a "Stem Cell Expert,Advisory Committee" ...
(Date:10/10/2008)..., Del., Oct. 10 /- AstraZen... and Drug Administration (FDA) has,approved once-d...-Release,Tablets for the acute treatment of the de..., the manic and mixed episodes associated with bip...bipolar I disorder as adjunctive,therapy to lithiu...
(Date:10/10/2008)... Md., Oct. 10 /- EntreMed, ...cal company developing,therapeutics for the treatm... today that Thomas H. Bliss, Jr., EntreMed,s Senio...nt, will present a Company,overview at the 16th An...ng,held October 12-14, 2008 in London, United King...
Breaking Biology Technology:Video: Boston Scientific Announces FDA Approval of Second-Generation TAXUS(R) Liberte(R) Drug-Eluting Stent 2Video: Boston Scientific Announces FDA Approval of Second-Generation TAXUS(R) Liberte(R) Drug-Eluting Stent 3Stem Cell Leader Beike Signs 8 New Cooperation Agreements, Establishes Safety Monitoring Boards 2Stem Cell Leader Beike Signs 8 New Cooperation Agreements, Establishes Safety Monitoring Boards 3FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 2FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 3FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 4FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 5FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 6FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 7FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 8FDA Approves AstraZeneca's SEROQUEL XR(R) for the Treatment of Bipolar Depression and Bipolar Mania 9EntreMed to Present at BioPartnering Europe Conference 2EntreMed to Present at BioPartnering Europe Conference 3
...ditor,s note: John Wade, VP/CIO, Saint Luke,s Heal...r at the annual Digital Healthcare Conference Ma...om his Kansas City office and offered a candid ass...ogy. , , WTN : What are the key remaining barrier..., , John Wade Wade: I think there are a couple...
...itor,s note: This is Part II of a three-part seri...Part I outlined the challenge facing businesses, a...n companies can do - and are doing - to address th...he estimates are accurate, about 10 percent of all... this year, and then the IT labor shortage will ki...
...When I last left you, it was in the middle of a we...ssion to open up a new trade office in that countr...both New and Old), the ancient capitol of Agra (ho...oved on to Mumbai (Bombay) for the last part of th...ons and key activities that the trade mission perf...
...Judy Lemke Green Bay, Wis. - Whenever the title..., the technologist usually is an established part ...ured out of retirement by Schneider National fol... , ,Lemke, executive vice president and CIO for th...n, logistics, and intermodal services, was recruit...
Other Biology Technology:Visions: Wade offers straight talk on e-medical records 2Visions: Wade offers straight talk on e-medical records 3Visions: Wade offers straight talk on e-medical records 4Visions: Wade offers straight talk on e-medical records 5IT workforce development: A little workforce planning goes a long way 2IT workforce development: A little workforce planning goes a long way 3IT workforce development: A little workforce planning goes a long way 4IT workforce development: A little workforce planning goes a long way 5A Midwest passage to India, Part II 2A Midwest passage to India, Part II 3A Midwest passage to India, Part II 4A Midwest passage to India, Part II 5A Midwest passage to India, Part II 6A Midwest passage to India, Part II 7A Midwest passage to India, Part II 8CIO Leadership Series: Judy Lemke, Schneider National 2CIO Leadership Series: Judy Lemke, Schneider National 3CIO Leadership Series: Judy Lemke, Schneider National 4CIO Leadership Series: Judy Lemke, Schneider National 5
RAB32, member RAS oncogene family, mRNA (cDNA clone MGC:13500 IMAGE:4280407), complete cds
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application. Each coverslip has 2.5 x 1 dimensions.
Recombinant Mouse TGF-beta RII/(human) Fc Chimera, CF
Human Norrin Biotinylated Affinity Purified PAb Protein Family: Other Growth Factors, Other Wnt-related Molecules
Biology Products: