Navigation Links
U-M researchers take new approach to defeating Gram-negative bugs

ve cells have sugars and carbohydrates on the outside in structures that look like hairs.

That exterior protection is part of what makes Gram-negative bacteria harder to kill antibiotics, Woodard said.

Woodard's team set out to genetically modify the cells to eliminate the key sugar to which the hair anchors on the outside of the cell.

"Unfortunately, the bug didn't die," Woodard said. The researchers found that a "backup" gene from a different pathway also could form the anchor, so they knocked out that gene, as well. Initially the cell with both genomic knockouts did not survive without special nutritional supplements. Later, they were surprised to see that with different growth conditions, the cell began to grow again but without the hair-like structure.

The cells survived---but they looked a lot like Gram-positive cells, without all the sugars on the outside.

"We, as well as the entire scientific community, always thought Gram-positive cells could not survive without this external structure. This shows that is not true," Woodard said. Though they didn't die, they were weakened, and that made the cells an easy target for antibiotics.

Because Woodard suspected he might be flying in the face of conventional wisdom on bacteria, he solicited second opinions from the Borstel Research Center in Germany, which does a good deal of work on Gram-negative bacteria. Scientists there were initially skeptical, he said, but eventually, Uwe Mamat and Buko Lindner from Borstel signed on to the project and became co-authors of the current paper.

Other members of the team were U-M medicinal chemistry doctoral students Timothy Meredith and Parag Aggarwal. Meredith, lead author of the publication, has since joined Harvard Medical School as a researcher.

Aggarwal, Mamat and Woodard continue to work on the approach, encouraged by the potential of developing a safer way to treat patients. They hope their research leads t
'"/>

Source:University of Michigan


Page: 1 2 3

Related biology news :

1. NYU researchers simulate molecular biological clock
2. Vital step in cellular migration described by UCSD medical researchers
3. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
4. UCSD researchers maintain stem cells without contaminated animal feeder layers
5. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
6. New protein discovered by Hebrew University researchers
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Agilent Technologies releases automated literature search tool for biology researchers
9. Self-assembled nano-sized probes allow Penn researchers to see tumors through flesh and skin
10. Yale researchers identify molecule for detecting parasitic infection in humans
11. US life expectancy about to decline, researchers say
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: researchers take new approach defeating Gram negative bugs

(Date:7/9/2014)... of increasing concern about the prevalence of antibiotic-resistant ... promising new pathway to disabling disease: blocking bacteria,s ... scientists showed how bacterial siderophore, a small molecule, ... fan bacterial growth as well as how ... infection process. Their findings appear in a recent ...
(Date:7/9/2014)... and fever but often can be defeated with antibiotics ... to beat. Now, scientists have built a new weapon against ... in the journal ACS Applied Materials & Interfaces , ... more of them than medicine alone. , David Leong, Jianping ... in wait, undetectable in the human body or in places ...
(Date:7/9/2014)... the amphibians with the highest distribution in the Iberian ... where it comes into contact with the red swamp ... by the Spaniard Germn Orizaola from the University of ... have developed a defensive response to the invasive species. ... they co-exist with the crayfish. , Numerous invasive organisms ...
Breaking Biology News(10 mins):Bacteria hijack plentiful iron supply source to flourish 2Frogs have developed rapid defences against the red swamp crayfish 2Frogs have developed rapid defences against the red swamp crayfish 3
... COLUMBIA, MO The American Association for the Advancement ... to Arun Chatterjee, a professor emeritus of plant sciences ... at the University of Missouri. Chatterjee was ... plant bacteriology, particularly for global regulation of virulence factors ...
... that the impact of disease-resistant genetically-modified wheat plants ... have looked at the effects of genetically-modified (GM) ... characterised by numerous insect species forming food webs. ... transgenic disease-resistant wheat lines and their effect on ...
... Madison, WI JANUARY 20, 2011 -- Chronic ... municipal wastewater, and atmospheric deposition from the burning of ... and harmful algal blooms. This nutrient pollution is prevalent ... Bay is the largest estuary in North America and ...
Cached Biology News:University of Missouri professor elected to American Association for the Advancement of Science 2New Anglo-Swiss research questions impact of GM wheat on insects 2Could oysters be used to clean up Chesapeake Bay? 2
(Date:1/15/2014)... England and SAN JOSE, California ... international biotechnology company developing antibody-drug conjugates for cancer, today announced ... Board of Directors. Dr Reynolds has over 20 years, development ... Medical Officer at Seattle Genetics. "I am delighted ...
(Date:1/15/2014)... 2014 More than 5 million Americans ... in 3 seniors will die with Alzheimer’s or another ... figures have shocked many Americans into looking for ways ... these tragic age-related cognitive disorders. Jonathan Weisman, president of ...
(Date:1/15/2014)... 2013 was a banner year of ... They saw continued independent research led by the team ... a $1 million grant from the Susanne Marcus Collins ... a peer reviewed journal, Amy Grant highlighted Brainwave Optimization® ...
(Date:1/14/2014)... 2014 Date: Friday, April 11, 2014 , ... Club, 1360 Almshouse Road, Warrington, Pa. , Details: ... dedicated to finding a cure for hepatitis B and improving ... its annual Crystal Ball on Friday, April 11 at Warrington ...
Breaking Biology Technology:Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2
... Danaher Corporation (NYSE:,DHR) announced that President and Chief ... at the J.P. Morgan Healthcare Conference in,San Francisco ... The audio will be,simultaneously webcast on http://www.danaher.com ... for one week., Danaher Corporation is ...
... THOR ), a world leader in products ... be participating in the JP,Morgan Healthcare Conference on ... chief executive officer, will provide an,update on the ... (11:30,a.m., Eastern Standard Time)., The presentation will ...
... than 500,000 Total Prescriptions Have Been Dispensed for ... ... 3 Shire plc (LSE: SHP,Nasdaq: SHPGY, TSX: SHQ), the ... from the U.S. Food and Drug,Administration (FDA) for three additional ...
Cached Biology Technology:FDA Approves Additional Dosage Strengths of Shire's ADHD Treatment VYVANSE(TM) (lisdexamfetamine dimesylate) Which May Help Physicians Tailor Treatment for Individual Patients 2FDA Approves Additional Dosage Strengths of Shire's ADHD Treatment VYVANSE(TM) (lisdexamfetamine dimesylate) Which May Help Physicians Tailor Treatment for Individual Patients 3FDA Approves Additional Dosage Strengths of Shire's ADHD Treatment VYVANSE(TM) (lisdexamfetamine dimesylate) Which May Help Physicians Tailor Treatment for Individual Patients 4FDA Approves Additional Dosage Strengths of Shire's ADHD Treatment VYVANSE(TM) (lisdexamfetamine dimesylate) Which May Help Physicians Tailor Treatment for Individual Patients 5
SDS-OutT SDS Precipitation Reagent...
... for fully automated systems for a broad ... configured for applications ranging from compound logistics, ... loop screening, which means it has fully ... picking and follow up studies all at ...
... Series of systems provide fast and reliable scalable automation ... development laboratories. Staccato systems are available in three base ... Series. ... All Staccato Application ...
Comp Dim: chamber L W D 45 45 0.8 mm chamber maximum volume 1500 μL...
Biology Products: