Navigation Links
Triplex assay used to assay duplex genomic DNA

Direct detection of base sequence in duplex nucleic acid has long been an unfulfilled objective. Ingeneus Research will publish "Heteropolymeric Triplex-Based Genomic Assay® to Detect Pathogens or Single-Nucleotide Polymorphisms in Human Genomic Samples" in the March 21st issue of the international, peer-reviewed, open access, online journal, PLoS ONE.

In the article they present a wealth of data relating to the assay of pathogens in samples also containing human genomic duplex DNA and to the assay of SNPs present in human genomic samples. The assays are carried out homogeneously and in solution at room temperature. Reactions can be monitored after as little as five minutes. The highly sensitive diagnostic assay allows for the direct detection of base sequence in human genomic duplex samples, thereby obviating the use of PCR which has inherent problems and is costly.

"We developed the heteropolymeric triplex assay step by step" says Jasmine Daksis, Senior Scientist with Ingeneus Research. "We started with synthetic 50-mer duplex targets and have developed our methods to the point where human genomic samples can be assayed." The assay uses YOYO-1, a bis-intercalator, to de-condense the duplex target, which renders the duplex nucleic acid readily reactive to oligo ssDNA probes. Any sequence present in the duplex may be specifically assayed. It is surmised that specific third strand binding creates additional grooves into which additional YOYO-1 molecules intercalate.

"We have decided not to focus on improving probe chemistry at this time, but rather to develop a flow injection based instrument which is matched to our chemistry," continued Daksis. Their Genome Flow® instrument, which employs hardware from FIALab Instruments of Bellevue, Washington, has one moving part, the syringe pump. It allows samples to be automatically quantitated, a necessary step in the Genomic Assay® because samples must be brought to a standard concentration, so they can be mixed with standard amounts of oligo probes for the purpose of automatic in solution assay. The instrument is easy to program, self-cleaning and inexpensive.

Daksis indicated that she expected to soon publish data on the use of the Genome Flow® instrument to carry out triplex assaying of genomic samples for pathogens or SNPs.
'"/>

Source:Public Library of Science


Related biology news :

1. PCRM develops worlds first cruelty-free insulin assay
2. Researchers develop assay that could be applied to drug screening
3. Molecule by molecule, new assay shows real-time gene activity
4. Stability of mRNA/DNA and DNA/DNA duplexes modulates mRNA transcription
5. Enzyme shown to help protect genomic stability
6. New comparative toxicogenomics database
7. Scientists document complex genomic events leading to the birth of new genes
8. Study of genomic DNA leads to new advances in cancer diagnostics
9. Measuring the impact of post-genomics on Mediterranean populations
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. PHACCS, an online tool for estimating the structure and diversity of uncultured viral communities using metagenomic information

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/13/2017)... April 13, 2017 According to a new market ... Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, ... Market is expected to grow from USD 14.30 Billion in 2017 to ... of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/11/2017)... , Apr. 11, 2017 Research and ... Market 2017-2021" report to their offering. ... The global eye tracking market to grow at a ... report, Global Eye Tracking Market 2017-2021, has been prepared based on ... covers the market landscape and its growth prospects over the coming ...
(Date:4/11/2017)... April 11, 2017 NXT-ID, Inc. (NASDAQ: ... company, announces the appointment of independent Directors Mr. Robin ... its Board of Directors, furthering the company,s corporate governance and ... Gino Pereira ... look forward to their guidance and benefiting from their considerable ...
Breaking Biology News(10 mins):
(Date:6/23/2017)... ... 2017 , ... The Academy of Model Aeronautics (AMA), the ... (UAA), the unifying voice for collegiate aviation education, are launching a joint UAS ... success through a STEM-based education platform. , Much like the program currently available ...
(Date:6/22/2017)... ... June 21, 2017 , ... RMC Pharmaceutical ... and engages Timothy Reinhardt to manage the new site. , Tim has 25 ... with his most recent role as the Director of Manufacturing and Supplier Quality ...
(Date:6/22/2017)... ... June 22, 2017 , ... ... specialists DST Diagnostische Systeme & Technologien GmbH, thereby expanding its product portfolio to ... from hay fever, urticaria, asthma, atopic eczema or a food allergy. Allergies are ...
(Date:6/22/2017)... ... 2017 , ... For the months of May and June, ... series on “Cell Therapy Regulation” for its regenerative medicine followship. The ... regulatory challenges of stem cell medical research. , Stem cell clinical trials present ...
Breaking Biology Technology: