Navigation Links
Triplex assay used to assay duplex genomic DNA

Direct detection of base sequence in duplex nucleic acid has long been an unfulfilled objective. Ingeneus Research will publish "Heteropolymeric Triplex-Based Genomic Assay® to Detect Pathogens or Single-Nucleotide Polymorphisms in Human Genomic Samples" in the March 21st issue of the international, peer-reviewed, open access, online journal, PLoS ONE.

In the article they present a wealth of data relating to the assay of pathogens in samples also containing human genomic duplex DNA and to the assay of SNPs present in human genomic samples. The assays are carried out homogeneously and in solution at room temperature. Reactions can be monitored after as little as five minutes. The highly sensitive diagnostic assay allows for the direct detection of base sequence in human genomic duplex samples, thereby obviating the use of PCR which has inherent problems and is costly.

"We developed the heteropolymeric triplex assay step by step" says Jasmine Daksis, Senior Scientist with Ingeneus Research. "We started with synthetic 50-mer duplex targets and have developed our methods to the point where human genomic samples can be assayed." The assay uses YOYO-1, a bis-intercalator, to de-condense the duplex target, which renders the duplex nucleic acid readily reactive to oligo ssDNA probes. Any sequence present in the duplex may be specifically assayed. It is surmised that specific third strand binding creates additional grooves into which additional YOYO-1 molecules intercalate.

"We have decided not to focus on improving probe chemistry at this time, but rather to develop a flow injection based instrument which is matched to our chemistry," continued Daksis. Their Genome Flow® instrument, which employs hardware from FIALab Instruments of Bellevue, Washington, has one moving part, the syringe pump. It allows samples to be automatically quantitated, a necessary step in the Genomic Assay® because samples must be brought to a standard concentration, so they
'"/>

Source:Public Library of Science


Page: 1 2

Related biology news :

1. PCRM develops worlds first cruelty-free insulin assay
2. Researchers develop assay that could be applied to drug screening
3. Molecule by molecule, new assay shows real-time gene activity
4. Stability of mRNA/DNA and DNA/DNA duplexes modulates mRNA transcription
5. Enzyme shown to help protect genomic stability
6. New comparative toxicogenomics database
7. Scientists document complex genomic events leading to the birth of new genes
8. Study of genomic DNA leads to new advances in cancer diagnostics
9. Measuring the impact of post-genomics on Mediterranean populations
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. PHACCS, an online tool for estimating the structure and diversity of uncultured viral communities using metagenomic information
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Triplex assay used assay duplex genomic DNA

(Date:7/30/2014)... the Entomological Society of America (ESA) has elected ten ... as a Fellow acknowledges outstanding contributions to entomology in ... or administration. The following Fellows will be recognized during ... will be held November 16-19, 2014 in Portland, Oregon. ... professor with the Department of Plant, Soil and Entomological ...
(Date:7/30/2014)... received a pledge of $1 million from the Cornelia ... new Neurobehavioral Biometry Center. , The newly ... the Laboratory to develop a greater breadth and depth ... of accelerating the pace of research to improve human ... premier resource for the Laboratory and its external partners, ...
(Date:7/30/2014)... shrinking ranges and a changing climate threaten some ... plants, and so conservationists establish seed collections to ... in hopes of preserving some genetic diversity., For ... simple models that offer a one-size-fits-all approach for ... saving 50 seed samples regardless of species, pollination ...
Breaking Biology News(10 mins):Entomological Society of America recognizes 2014 fellows 2Entomological Society of America recognizes 2014 fellows 3Entomological Society of America recognizes 2014 fellows 4Entomological Society of America recognizes 2014 fellows 5Entomological Society of America recognizes 2014 fellows 6Entomological Society of America recognizes 2014 fellows 7Entomological Society of America recognizes 2014 fellows 8Entomological Society of America recognizes 2014 fellows 9Entomological Society of America recognizes 2014 fellows 10Entomological Society of America recognizes 2014 fellows 11Entomological Society of America recognizes 2014 fellows 12Entomological Society of America recognizes 2014 fellows 13Entomological Society of America recognizes 2014 fellows 14Entomological Society of America recognizes 2014 fellows 15Entomological Society of America recognizes 2014 fellows 16Entomological Society of America recognizes 2014 fellows 17Rossi Foundation pledges $1M for JAX neurobehavioral research center 2Saving seeds the right way can save the world's plants 2
... to biobanks vast collections of human tissue samples that ... have a right to basic information about how their donations ... a new paper. The idea behind biobanks is that ... to medical records and other health information, can yield discoveries ...
... and terrorist plots are usually the preserve of Hollywood ... is the stuff of real life. As controversy about ... new paper argues for a complete overhaul of current ... Reconfiguring Global Responses to Influenza, by Dr Paul Forster, ...
... modify their body movements to be in tune with others, ... to be in synchrony with their peers. For example, we ... unison at the end of a concert. This phenomenon is ... the RIKEN Brain Science Institute report today that pairs of ...
Cached Biology News:Safeguards needed for tissue donors 2Pandemic controversies: The global response to pandemic influenza must change 2Primates too can move in unison 2
(Date:7/30/2014)... Diego, CA (PRWEB) July 30, 2014 ... announced their weighing boat special – Buy ... 1 free. This promotion will benefit lab technicians looking ... work. Weighing boats are ideal for multiple applications including ... offers a variety of choices including diamond-shaped, hexagonal, square, ...
(Date:7/29/2014)... “PharmaSphere: Emerging Biotech Investment Report ... Funding” report provides in-depth analysis of the current ... venture capital segments of the financial industry, bearing ... significant impact on the future of emerging biotechnology ... report identifies regions of the world that are ...
(Date:7/29/2014)... , July 29, 2014 Human Longevity, ... therapeutic company focused on extending the healthy, high performance ... Franz Och , Ph.D., an expert in machine learning ... Och comes to HLI from Google where he was ... He will report directly to HLI Co-Founder and CEO, ...
(Date:7/29/2014)... , July 29, 2014  The Society ... new and updated program for the 9 th ... 12, 2014 in Amelia Island , Florida.  ... and investigators remains unchanged, however, the topics and ... and networking opportunity among all stakeholders with a ...
Breaking Biology Technology:Weighing Boat Promotion Now Available at Pipette.com, Major Pipette Distributor 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 3Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 4Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 3
... OpGen , a company that makes optical tests capable ... the final stage of its $5 million investment round. ... Wisconsin-Madison technology, already sells its testing services to a ... between marketing that service and new developments such as ...
... toilet seat. Seriously. The Brondell Swash 600. But then controversy ... toilet seat in the first place. , ,Last fall I ... This group, organized by networker extraordinaire and Stonebrick Group founder ... virtue of their work or their own networking prowess, presumably ...
... Jim Doyle has appointed Mary Burke, who was most recently ... Bicycles , to be the state's next commerce secretary starting ... end of 2004 to take a job with a law ... Doyle's business-plan contest will stop accepting submissions after January 31. ...
Cached Biology Technology:OpGen completes $5 million investment round 2The myth of objectivity 2The myth of objectivity 3
Mouse monoclonal [TU-20] to beta III Tubulin (FITC) ( Abpromise for all tested applications). entrezGeneID: 10381 SwissProtID: Q13509...
Sheep polyclonal to Methaqualone ( Abpromise for all tested applications). Antigen: Methaqualone(5') conjugated to BSA...
Mouse monoclonal [CP23] to Ricin communis ( Abpromise for all tested applications)....
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
Biology Products: