Navigation Links
Triplex assay used to assay duplex genomic DNA

Direct detection of base sequence in duplex nucleic acid has long been an unfulfilled objective. Ingeneus Research will publish "Heteropolymeric Triplex-Based Genomic Assay® to Detect Pathogens or Single-Nucleotide Polymorphisms in Human Genomic Samples" in the March 21st issue of the international, peer-reviewed, open access, online journal, PLoS ONE.

In the article they present a wealth of data relating to the assay of pathogens in samples also containing human genomic duplex DNA and to the assay of SNPs present in human genomic samples. The assays are carried out homogeneously and in solution at room temperature. Reactions can be monitored after as little as five minutes. The highly sensitive diagnostic assay allows for the direct detection of base sequence in human genomic duplex samples, thereby obviating the use of PCR which has inherent problems and is costly.

"We developed the heteropolymeric triplex assay step by step" says Jasmine Daksis, Senior Scientist with Ingeneus Research. "We started with synthetic 50-mer duplex targets and have developed our methods to the point where human genomic samples can be assayed." The assay uses YOYO-1, a bis-intercalator, to de-condense the duplex target, which renders the duplex nucleic acid readily reactive to oligo ssDNA probes. Any sequence present in the duplex may be specifically assayed. It is surmised that specific third strand binding creates additional grooves into which additional YOYO-1 molecules intercalate.

"We have decided not to focus on improving probe chemistry at this time, but rather to develop a flow injection based instrument which is matched to our chemistry," continued Daksis. Their Genome Flow® instrument, which employs hardware from FIALab Instruments of Bellevue, Washington, has one moving part, the syringe pump. It allows samples to be automatically quantitated, a necessary step in the Genomic Assay® because samples must be brought to a standard concentration, so they can be mixed with standard amounts of oligo probes for the purpose of automatic in solution assay. The instrument is easy to program, self-cleaning and inexpensive.

Daksis indicated that she expected to soon publish data on the use of the Genome Flow® instrument to carry out triplex assaying of genomic samples for pathogens or SNPs.
'"/>

Source:Public Library of Science


Related biology news :

1. PCRM develops worlds first cruelty-free insulin assay
2. Researchers develop assay that could be applied to drug screening
3. Molecule by molecule, new assay shows real-time gene activity
4. Stability of mRNA/DNA and DNA/DNA duplexes modulates mRNA transcription
5. Enzyme shown to help protect genomic stability
6. New comparative toxicogenomics database
7. Scientists document complex genomic events leading to the birth of new genes
8. Study of genomic DNA leads to new advances in cancer diagnostics
9. Measuring the impact of post-genomics on Mediterranean populations
10. Genrate: a generative model that finds and scores new genes and exons in genomic microarray data
11. PHACCS, an online tool for estimating the structure and diversity of uncultured viral communities using metagenomic information

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/20/2017)... (NYSE: DAL ) customers now can use fingerprints instead of ... Airport (DCA). ... Delta launches biometrics to board aircraft at Reagan Washington National Airport ... Delta,s biometric boarding pass experience that launched in May ... boarding process to allow eligible Delta SkyMiles Members who are enrolled in ...
(Date:6/23/2017)... ARMONK, N.Y. and ITHACA, N.Y. ... IBM ) and Cornell University, a leader in dairy ... combined with bioinformatics designed to help reduce the chances ... breaches. With the onset of this dairy project, Cornell ... the Consortium for Sequencing the Food Supply Chain, a ...
(Date:5/23/2017)... 2017  Hunova, the first robotic gym for the rehabilitation and functional ... in Genoa, Italy . The first 30 robots will ... USA . The technology was developed and patented at ... IIT spin-off Movendo Technology thanks to a 10 million euro investment from ... click: ...
Breaking Biology News(10 mins):
(Date:8/10/2017)... ... August 09, 2017 , ... SPIE, the ... laboratories — the Wellman Center for Photomedicine, the Manstein Lab in the Cutaneous ... Beckman Laser Institute at University of California, Irvine — and the Hillenkamp family ...
(Date:8/10/2017)... (PRWEB) , ... August 10, 2017 , ... ... the stock market news outlet had initiated coverage on Next Group Holdings, Inc. ... and underserved consumer markets geared toward those that cannot engage in traditional banking ...
(Date:8/8/2017)... ... August 08, 2017 , ... HumanZyme ... in human cells, today announced the launch of HumanKine® Interferon beta ... I family of interferons that activate Th1-type innate immune responses against viral and bacterial ...
(Date:8/8/2017)... ... 08, 2017 , ... Myoderm , a global clinical trial drug supply ... announcement comes on the heels of the opening of Myoderm’s European warehouse facility earlier ... to adding staff and establishing operations to support its customers around the world. ...
Breaking Biology Technology: