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The connector rotation hypothesis? Phage do not pack their DNA by rotation

y would have seen a sine wave-like fluctuation in intensity in both channels with a phase shift of 90 degrees. They did not see the sine waves, and mathematical analysis of the fluorescence pattern confirmed that the changes in the fluorescence emitted by the molecules as packaging took place did not correspond to any sort of continuous rotational motion. The researchers concluded with more than 99% certainty that the packaging mechanism does not involve rotation.

How, then, does it happen? The researchers noted that their findings are compatible with a recently proposed nonrotating model in which the ring of ATPases alternately compresses and extends, drawing in the DNA. But further testing will be needed to confirm the validity of that model to the degree of certainty with which this team rejected the rotator hypothesis.


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Source:Public Library of Science


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