Navigation Links
The connector rotation hypothesis? Phage do not pack their DNA by rotation

The life cycles of many viruses include a self-assembly stage in which a powerful molecular motor must pack the DNA genome into the virus's preformed shell (the capsid). How it manages this intricate feat has been subject to debate, but we know that the DNA passes into the capsid shell through a channel formed by a structure called the connector. Scientists have speculated that rotation of the connector complex might feed the DNA into the capsid as it turns.

In a new study published online this week in the open access journal PLoS Biology, researchers Thorsten Hugel, Jens Michaelis, Craig Hetherington, and Carlos Bustamante present their detailed investigation into how the Bacillus subtilis bacteriophage ö29 crams its DNA into the capsid following replication.

Using their innovative system, Hugel and colleagues were able to observe capsids as they pack their DNA to directly test the connector rotation hypothesis. They found that it is more than likely not the mechanism by which the DNA is packed. The researchers combined single-molecule fluorescence polarization with "magnetic tweezers" to glue the end of the capsid farthest from the hole to a slide using antibodies, and then they draw out the DNA being packaged in the opposite direction by attaching a magnetic bead to its loose end and applying a magnetic field. Importantly, they also labeled the connector complex with fluorescent dye molecules so they could observe its motion using single-molecule fluorescence polarization spectroscopy.

The researchers then looked at connector movement during DNA packaging in six ö29 mutants. After attaching the fluorescent molecules to the connector complex, they set the mutants to work packaging DNA in a flow chamber. As the connectors functioned, the researchers shone homogeneously polarized light on them and recorded the pattern of fluorescence produced in two channels at right angles to one other.

If the connectors had been rotating, the
'"/>

Source:Public Library of Science


Page: 1 2

Related biology news :

1. Priming embryonic stem cells to fulfill their promise
2. Protein offers way to stop microscopic parasites in their tracks
3. Flocking together: Study shows how animal groups find their way
4. Where bacteria get their genes
5. Chickadees can help humans get their bearings
6. Bacteria use hosts immune response to their competitive advantage
7. Structures of marine toxins provide insight into their effectiveness as cancer drugs
8. Beauty queens urge girls not to sacrifice their bones
9. Researchers learn how blood vessel cells cope with their pressure-packed job
10. Stem cells electric abilities might help their safe clinical use
11. Multiple genes permit closely related fish species to mix and match their color vision
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: The connector rotation hypothesis Phage not pack their DNA rotation

(Date:7/11/2014)... to study the proteins that let nerve cells send ... stumbled upon a biological tactic that may offer a ... a safe and environmentally responsible way. , Their ... one species and harmless for a closely related onesuggests ... without harming beneficial species like bees. A summary of ...
(Date:7/10/2014)... Fe, Albuquerque, and other major cities in New Mexico, ... treated municipal wastewater rather than precious potable water supplies. ... 40% of all golf courses receive treated effluent. Reusing ... , Additionally, golf courses and homeowners alike fertilize their ... fertilizer is nitrate. A New Mexico State University turfgrass ...
(Date:7/10/2014)... , June 27, 2014  Pomerantz LLP ... Biopharmaceuticals, Inc. ("Provectus" or the "Company")(NYSE-MKT: PVCT) and ... in United States District Court, Middle District of ... on behalf of a class consisting of all ... Provectus securities between December 17, 2013 and May ...
Breaking Biology News(10 mins):Potent spider toxin 'electrocutes' German, not American, cockroaches 2Potent spider toxin 'electrocutes' German, not American, cockroaches 3'Tailored' water -- the latest in lawn care 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3
... fathers who are active in their children,s upbringing can significantly ... Now, a new study by University of South Florida ... born may play an important role in preventing death during ... is black. The USF team sought to evaluate ...
... RICHMOND, Va. , June 17 Commercializing University Technology ... 29 presented by LeClairRyan and a panel of industry experts from ... , , ... rise again, entrepreneurs, start-ups and emerging businesses should investigate all options to ...
... gestures have intentional meaning and are made with the ... and Richard Byrne from the University of St. Andrews ... communication takes a significant step forward with the authors, ... gestural communication of non-humans, applied here to a group ...
Cached Biology News:Father involvement in pregnancy could reduce infant mortality 2Free Webinar to Focus on Commercializing University Technology 2Free Webinar to Focus on Commercializing University Technology 3Signal like you mean it 2
(Date:7/11/2014)... 11, 2014  Researchers have pioneered a revolutionary ... new technology, called Virtual Finger, allows scientists to ... neurons and synapses using the flat surface of ... 3D imaging studies orders of magnitude more efficient, ... level across many areas of experimental biology. The ...
(Date:7/10/2014)... Cleveland, Ohio (PRWEB) July 10, 2014 ... in ion channel-expressing cell lines, and the gap that ... new cells that expand its industry-leading portfolio of validated ... focuses on ion channel-expressing cell lines, as ion channels ... able to control this activity creates the potential to ...
(Date:7/10/2014)... 10, 2014 EvoDerma ’s NOOME Anti-Aging ... rejuvenating device now comes with a second treatment cup to ... , The new cup is thinner on the edges for ... target rough, thin and uneven surfaces on the face, neck ... a stimulating treatment on areas such as the cheeks and ...
(Date:7/10/2014)... July 10, 2014 /PRNewswire-iReach/ -- Tute Genomics, ... today announced an agreement with Lineagen, Inc., ... Lineagen,s NextStep Dx PLUS.  Lineagen, focused ... neurological disorders, currently offers FirstStep Dx ... service used by healthcare providers as a ...
Breaking Biology Technology:Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4
... a Financial Crisis, Draws Regional Biopharmaceutical Executives, Investment Leaders ... Biotech companies throughout the country are reeling from the ... funds slated to run out of cash before 2010, ... a life sciences symposium presented by Yankee ...
... Spectral Diagnostics Inc. (TSX:SDI), a company developing products ... findings demonstrating the Toraymyxin(TM) cartridge, a blood purification ... added to conventional therapy, significantly improved hemodynamics and ... with severe sepsis and septic shock. The article ...
... can anticipate a future event and prepare for it, ... Science. In a paper that appeared today in ... and research associate Dr. Orna Dahan of the Institute,s ... Gal Romano of Tel Aviv University, examined microorganisms living ...
Cached Biology Technology:Life Science Leaders Discuss Strategies for Surviving the Financial Crisis at Yankee Public Relations Symposium 2Life Science Leaders Discuss Strategies for Surviving the Financial Crisis at Yankee Public Relations Symposium 3Life Science Leaders Discuss Strategies for Surviving the Financial Crisis at Yankee Public Relations Symposium 4Life Science Leaders Discuss Strategies for Surviving the Financial Crisis at Yankee Public Relations Symposium 5Spectral announces publication demonstrating Toraymyxin(TM) significantly reduces mortality from sepsis in prospective controlled trial 2Spectral announces publication demonstrating Toraymyxin(TM) significantly reduces mortality from sepsis in prospective controlled trial 3Israeli scientists show bacteria can plan ahead 2Israeli scientists show bacteria can plan ahead 3
...
...
... System is an imaging based plate ... and high throughput screening which can ... The Hamamatsu light sources and cameras ... decades-long experience in photon detection technology. ...
... pCITE-2a-c(+) vectors are designed for enhanced translation of ... e.g. rabbit reticulocyte lysate. The maps for pCITE-2b(+) ... the following exceptions: pCITE-2b(+) is a 3793 bp ... I and Xcm I sites. Subtract 7 bp ...
Biology Products: