Navigation Links
Technology reveals 'lock and key' proteins behind diseases

A new technology developed at the University of Toronto is revealing biochemical processes responsible for diseases such as cystic fibrosis and could one day pave the way for pharmaceutical applications.

A study appearing in the April 13 issue of Molecular Cell describes how U of T and Johns Hopkins University researchers designed a device to test for proteins that play an important role in human health and disease. The technology, iMYTH (or integrated membrane yeast-two hybrid system), scans cells to detect proteins that interact with key proteins called ATP-binding cassette (ABC) transporters ?proteins that, when impaired, can cause disease. One of the best known ABC transporters is the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), which, when disabled by mutation, causes cystic fibrosis, a hereditary disease that results in progressive disability and early death. Another important ABC protein is the Multidrug Resistance Protein (MRP), which normally removes drug metabolites and toxins from cells in our bodies but when overzealous can contribute to the drug resistance of tumours, thereby thwarting chemotherapy. __IMAGE_2

"All the cells in our bodies contain transporters that are poised in cellular membranes and act as ‘gatekeepers?to allow the entry of certain substances, like nutrients, into the cell and promote the export of other substances, like toxins, out of the cell," says Professor Igor Stagljar, Department of Medical Genetics and Department of Biochemistry at the University of Toronto and lead author of the study. "When the function of these transporters is impaired, disease can result. This device gives us insights as to what proteins are interfering with this process."

iMYTH works by scanning cells to reveal proteins that fit with the transporters, the only screening system sophisticated enough to work with delicate membrane proteins. Simply, if two proteins interact in iMYTH, they will stain the yeast cell bl ue. "Like lock and key, if two proteins interact with one another, it is safe to assume they participate or regulate the same cellular process," explains Stagljar. "Identifying new interactors for ABC transporters may reveal unanticipated aspects of how these transporters function and help researchers gain clues for fighting disease and drug resistance."

Using iMYTH, the Stagljar lab identified six proteins that interact with and presumably communicate with the ABC transporter Ycf1p, a yeast version of the human proteins CFTR and MRP. These newly discovered protein interactors represent novel potential pharmaceutical targets. Through a series of biochemical and genetic tests, the researchers discovered that one of these interactors, Tus1p, regulates Ycf1p transporter function in a completely novel way to stimulate its ability to remove toxins from the cell.

"The more we learn about membrane proteins, the better we can use this knowledge for pharmacological and clinical applications," Stagljar says. "We work by putting together biochemical processes piece by piece like a puzzle. Hopefully soon we will have a complete picture of how many other diseases such as breast cancer, heart diseases, arthritis and schizophrenia are caused by mutations in various human membrane proteins."
'"/>

Source:University of Toronto


Related biology news :

1. Breakthrough Microarray-based Technology for the Study of Cancer
2. Affymetrix Licenses Microfluidics Technology From Caliper Life Sciences
3. Affymetrix and bioMerieux Extend Their Agreement on GeneChip(R) Technology to Breast Cancer Diagnostics
4. Technology for monitoring fetal oxygen during labor offers no apparent benefit
5. Technology exists to keep E. coli out of food
6. A bacterial genome reveals new targets to combat infectious disease
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Examination of internal wiring of yeast, worm, and fly reveals conserved circuits
9. NYU study reveals how brains immune system fights viral encephalitis
10. Study reveals new technique for fingerprinting environmental samples
11. Study reveals dramatic difference between breast cancers in US and Africa

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/14/2017)... -- IBM (NYSE: IBM ) is introducing several innovative partner ... developing collaboration between startups and global businesses, taking place in ... event, nine startups will showcase the solutions they have built ... France is one of ... 30 percent increase in the number of startups created between ...
(Date:5/16/2017)... --  Bridge Patient Portal , an enterprise patient ... Systems , an electronic medical record solutions developer ... a partnership to build an interface between the ... products, including Centricity Practice Solution (CPS), Centricity Business ... integrations will allow healthcare delivery networks using GE ...
(Date:4/19/2017)... April 19, 2017 The global ... landscape is marked by the presence of several large ... held by five major players - 3M Cogent, NEC ... accounted for nearly 61% of the global military biometric ... in the global military biometrics market boast global presence, ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... They call it ... biological network, a depiction of a system of linkages and connections so complex ... associate professor of computer science at Worcester Polytechnic Institute (WPI) and director of ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... with the addition of its newest module, US Hemostats & Sealants. , SmartTRAK’s ... hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic sealants used in surgical ...
(Date:10/11/2017)... YORBA LINDA, CA (PRWEB) , ... October 11, ... ... adapted to upregulate any gene in its endogenous context, enabling overexpression experiments and ... activation (CRISPRa) system with small RNA guides is transformative for performing systematic gain-of-function ...
(Date:10/11/2017)... 11, 2017  VMS BioMarketing, a leading provider of patient ... Clinical Nurse Educator (CNE) network, which will launch this week. ... among health care professionals to enhance the patient care experience ... and other health care professionals to help women who have ... ...
Breaking Biology Technology: