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Technique speeds up detecting, treating wound bacteria

For Dr. Sydney Finegold, research is like reading a really good mystery or detective story.

"But it is real life and one can see the results in one's own patients," he said. "So, one can have great fun while accomplishing worthwhile things."

With a grant from the Department of Defense, Finegold has taken his passion for research and applied it to a problem that affects civilians as well as injured service members: wound bacteria.

"The flora of wound infections is very complex," he said. "At times there can be 12 or more organisms present, and most clinical laboratories are not proficient in isolating and identifying anaerobes, which often predominate."

Using DNA detection methods though a technique called real-time polymerase chain reaction, the physician-researcher from the West Los Angeles Veterans Administration Medical Center can drastically cut the time it takes for lab personnel to figure out just what bacteria they're dealing with.

"The big advantage of real-time PCR is that we get quantitative information and accurate identification on the organisms in five hours or so, whereas the current procedure--culturing and identifying organisms by biochemical activity, etc.--can take one to several days and sometimes weeks, depending on the organism," he said.

His technique is also useful in detecting flora that can't easily be grown in culture because no one's been able to determine just what the bacteria like in the way of nutrients and environmental conditions.

The earlier the lab staff has answers, the earlier the correct treatment can begin. Initial treatment is necessarily empiric.

"When the patient is quite ill, clinicians necessarily use a broad spectrum (antibiotic), hoping not to overlook anything," Finegold said. "The resulting overuse of antibiotics definitely contributes to antibiotic resistance."

According to the Centers for Disease Control and Prevention Web site, antibioti
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Source:US Department of Defense Congressionally Directed Medical Research Programs


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