Navigation Links
Swiss researchers develop all-in-one remote control gene expression tool

In an article appearing online today in the journal Nature Methods, researchers at the EPFL (Ecole Polytechnique Fédérale de Lausanne) unveil a powerful new tool that will facilitate genetic research and open up new avenues for the clinical treatment of genetic disease.

The researchers have combined several gene manipulation techniques and incorporated them into a single lentiviral vector ?a gene delivery system partly derived from HIV. When injected into living cells ?either in vitro or in vivo ?the genetic material aboard the lentiviral vector joins the genetic material in the nucleus of the cell, causing the cell to express the protein encoded by the new gene. This versatile package can also carry bits of RNA that stop the cell from expressing one of its own genes, by way of RNA interference. But the cargo that makes this tool really novel and exciting is a fusion protein that acts as a kind of remote control. By administering an antibiotic, the genetic manipulation ?either the transgenic material introduced by the lentivirus, or the gene silencing via RNA interference--can be switched on or off at will.

An all-in-one tool like this ?efficiently combining techniques that each previously required separate delivery ?will likely see wide use in genetic research and in clinical gene therapy applications. It is particularly applicable for use in stem cells, embryonic cells and tissues and organs that are amenable to genetic transduction.

"It's a flexible way to regulate the expression of genes in a cell," says EPFL professor Didier Trono. "The lentiviral vector integrates in an irreversible fashion into the cell and is then part of its genetic cargo and part of the genetic cargo of all its progeny."

The efficiency of the lentiviral vector will make it easier to create transgenic animals used in studying human genetic diseases such as Parkinson's, Alzheimer's or Huntington's diseases. The expression of the gene can be turned on and off
'"/>

Source:Ecole Polytechnique F茅d茅rale de Lausanne


Page: 1 2

Related biology news :

1. NYU researchers simulate molecular biological clock
2. Vital step in cellular migration described by UCSD medical researchers
3. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
4. UCSD researchers maintain stem cells without contaminated animal feeder layers
5. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
6. New protein discovered by Hebrew University researchers
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Agilent Technologies releases automated literature search tool for biology researchers
9. Self-assembled nano-sized probes allow Penn researchers to see tumors through flesh and skin
10. Yale researchers identify molecule for detecting parasitic infection in humans
11. US life expectancy about to decline, researchers say
Post Your Comments:
(Date:7/28/2014)... of a new study suggest two ways to effectively address ... well in obese women, compared to women of a normal ... method, researchers say, but as the population has increased in ... for obese women. Studies have consistently found that obesity has ... may in turn affect how well the pill prevents pregnancy. ...
(Date:7/28/2014)... himself the bug hunter, but the target of his ... and identified with special methods and instruments. Benjamin Hause, ... Diagnostic Laboratory at Kansas State University, recently published an ... which is an important find in the United States. ... didn,t know what it was," Hause said. "We used ...
(Date:7/28/2014)... HOUSTON (July 28, 2014) Rice University researchers ... chains of varying flexibility that can be used as ... visual proof that "bead-spring" polymers, introduced as theory in ... flexible as required and should be of interest to ... , The work led by Rice chemical and biomolecular ...
Breaking Biology News(10 mins):Strategies identified to improve oral contraceptive success with obese women 2Researcher using next-generation sequencing to rapidly identify pathogens 2Researcher using next-generation sequencing to rapidly identify pathogens 3Seeing is bead-lieving 2
... new gene could lead to better bug-resistant plants. ... the cover of this week,s Proceedings of the ... could re-learn a thing or two from their wild ... losing beneficial traits common to wild tomatoes. Anthony Schilmiller, ...
... Sept. 17, 2012Whether your neighbourhood is conducive to ... according to a new study by researchers at ... Evaluative Sciences. Researchers found this risk was ... neighbourhoods. A new immigrant living in a less ...
... study suggests there are two root causes of a type ... already have sparked a clinical trial, may guide development of ... CF. Almost half of patients with CF will ... develop it in their teens. In addition to the health ...
Cached Biology News:New gene could lead to better bug-resistant plants 2Risk of developing diabetes higher in neighborhoods that aren't walk-friendly: Study 2Cystic fibrosis disrupts pancreas two ways in CF-related diabetes 2Cystic fibrosis disrupts pancreas two ways in CF-related diabetes 3
(Date:7/29/2014)... 29, 2014 Human Longevity, Inc. (HLI), a ... on extending the healthy, high performance human life span, ... Ph.D., an expert in machine learning and machine translation ... HLI from Google where he was Distinguished Research Scientist ... directly to HLI Co-Founder and CEO, J. Craig ...
(Date:7/29/2014)... , July 29, 2014  Pomerantz LLP is investigating ... ("Impax" or the "Company") (NASDAQ: IPXL).  Such investors are ... rswilloughby@pomlaw.com or 888-476-6529, ext. 237. ... officers and/or directors have violated Sections 10(b) and 20(a) ... On July 29, 2014, Impax Laboratories, Inc. (the ...
(Date:7/29/2014)... Oregon , July 29, 2014 ... "Global surgical equipment market (types, applications and geography) - ... Research, Report, Opportunities, Segmentation and Forecast, 2013 - 2020", ... to reach $12.1 billion by 2020 registering a CAGR ... to the breakthrough achieved by the electro surgical devices ...
(Date:7/29/2014)... July 29, 2014 Philadelphia’s newest entrepreneurial incubation ... get their start. The Innovation Center @3401 , a ... University City Science Center to help attract and nurture ... Monday, July 28. , The Innovation Center ... and Market Streets, is the new home of DreamIt ...
Breaking Biology Technology:Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3SHAREHOLDER ALERT: Pomerantz Law Firm Investigates Claims On Behalf of Investors of Impax Laboratories, Inc. - IPXL 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 3Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 2Innovation Center @3401 Opens for Business; DreamIt Ventures and Point.io First Residents 3
... Global consultant Tom Koulopoulos said at the Fusion2006 CEO-CIO ... work they do that does not support their core competency ... expect, a discussion of outsourcing versus doing work in house ... the Delphi Group founder to tap his experience in IT ...
... Robert Paulos, associate director for the IceCube project, holds ... IceCube neutrino telescope. Photo courtesy of the Ice Cube ... of scientists and engineers has taken a major step ... cosmic neutrino observatory, harnessing a sophisticated hot-water drill to ...
... have been infected with the H5N1 avian influenza virus, mostly ... virus does not spread easily from its pioneering human hosts ... a study of cells in the human respiratory tract reveals ... that makes it difficult for the virus to jump from ...
Cached Biology Technology:IT outsourcing according to Tom Koulopoulos, part 1 2IT outsourcing according to Tom Koulopoulos, part 1 3Polar neutrino observatory construction leaps ahead 2Polar neutrino observatory construction leaps ahead 3Polar neutrino observatory construction leaps ahead 4UW shows why avian flu hasn't spread among humans yet 2UW shows why avian flu hasn't spread among humans yet 3
... Agilents G1979A multisignal output accessory ... multiple data systems in laboratories with ... unprocessed digital MS signals from Agilent ... into analog format and transmits the ...
... DSQ II (Dual-Stage Quadrupole) Thermo Scientific ... leader in analytical instrumentation, introduces the latest ... DSQ II. Based on the proven reliability ... PolarisQ GC/MS systems, the DSQ II incorporates ...
... the macro- and micronutrients as ... (1962) and the vitamins as ... (1968). Preparation Quantity Equivalency: Formulated ... powder per liter of medium. ...
... Contents include: Microarray hybridization chamber ... chamber top, clamp, thumbscrew) Microarray chamber user ... and are intended to be cleaned and ... with Agilents Catalog Oligo Microarray kits and ...
Biology Products: