Navigation Links
Swiss researchers develop all-in-one remote control gene expression tool

In an article appearing online today in the journal Nature Methods, researchers at the EPFL (Ecole Polytechnique Fédérale de Lausanne) unveil a powerful new tool that will facilitate genetic research and open up new avenues for the clinical treatment of genetic disease.

The researchers have combined several gene manipulation techniques and incorporated them into a single lentiviral vector ?a gene delivery system partly derived from HIV. When injected into living cells ?either in vitro or in vivo ?the genetic material aboard the lentiviral vector joins the genetic material in the nucleus of the cell, causing the cell to express the protein encoded by the new gene. This versatile package can also carry bits of RNA that stop the cell from expressing one of its own genes, by way of RNA interference. But the cargo that makes this tool really novel and exciting is a fusion protein that acts as a kind of remote control. By administering an antibiotic, the genetic manipulation ?either the transgenic material introduced by the lentivirus, or the gene silencing via RNA interference--can be switched on or off at will.

An all-in-one tool like this ?efficiently combining techniques that each previously required separate delivery ?will likely see wide use in genetic research and in clinical gene therapy applications. It is particularly applicable for use in stem cells, embryonic cells and tissues and organs that are amenable to genetic transduction.

"It's a flexible way to regulate the expression of genes in a cell," says EPFL professor Didier Trono. "The lentiviral vector integrates in an irreversible fashion into the cell and is then part of its genetic cargo and part of the genetic cargo of all its progeny."

The efficiency of the lentiviral vector will make it easier to create transgenic animals used in studying human genetic diseases such as Parkinson's, Alzheimer's or Huntington's diseases. The expression of the gene can be turned on and off by feeding the animal an antibiotic. Likewise genes that express pathogenic proteins can be conditionally silenced, allowing researchers to study possible new therapeutic approaches.

In cancer research this tool could be used to study gene function in tumor cells and for generating in vivo tumor models for drug screening and delivery.

In another application, dying cells (such as neurons) can be rescued by introducing a gene that expresses a growth factor. Thanks to the remote control carried in the lentivirus the expression of this growth factor can now be turned off when the desired effect is achieved, thus preventing unharnessed growth ?otherwise known as cancer.

"It's an extremely polymorphic tool, useful in testing therapies and in preclinical studies," says Trono. "Using it we can control the gene expression in vivo in an extraordinary and sensitive way."


'"/>

Source:Ecole Polytechnique F茅d茅rale de Lausanne


Related biology news :

1. NYU researchers simulate molecular biological clock
2. Vital step in cellular migration described by UCSD medical researchers
3. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
4. UCSD researchers maintain stem cells without contaminated animal feeder layers
5. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
6. New protein discovered by Hebrew University researchers
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Agilent Technologies releases automated literature search tool for biology researchers
9. Self-assembled nano-sized probes allow Penn researchers to see tumors through flesh and skin
10. Yale researchers identify molecule for detecting parasitic infection in humans
11. US life expectancy about to decline, researchers say
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/20/2017)... , March 20, 2017 At this year,s ... -based biometrics manufacturer DERMALOG. The Chancellor came to the DERMALOG stand ... is this year,s CeBIT partner country. At the largest German biometrics ... in use: fingerprint, face and iris recognition as well as DERMALOG´s multi-biometrics ... ...
(Date:3/16/2017)... - Against identity fraud with DERMALOG solutions "Made in Germany "  ... ... multi-biometric solutions provide a crucial contribution against identity fraud. (PRNewsFoto/Dermalog Identification Systems) ... Used combined in one project, multi-biometric solutions provide a crucial contribution against identity ... ...
(Date:3/9/2017)... , March 9, 2017 4Dx has ... World Lung Imaging Workshop at the University of Pennsylvania. ... invited to deliver the latest data to world leaders ... event brings together leaders at the forefront of the ... lung imaging. "The quality of the ...
Breaking Biology News(10 mins):
(Date:5/23/2017)... ... May 23, 2017 , ... ... as Vice President of Clinical Operations. She brings years of expertise in establishing ... From her professional foundation as a licensed occupational therapist, through a variety of ...
(Date:5/23/2017)... ... May 23, 2017 , ... Customers ... their control technology again and again. METTLER TOLEDO has released two new videos ... videos illustrate how integration of the ACT350 into Siemens and Allen Bradley PLCs ...
(Date:5/22/2017)... ... May 22, 2017 , ... ... is exhibiting in booth B2 at the Association for Pathology Informatics Annual ... , In addition to demonstrating its Cancer Diagnostic Cockpit and Consultation Portal, Inspirata ...
(Date:5/19/2017)... ... May 19, 2017 , ... In response to the strong base of ... Systems, Inc. announces the release of their Gait Trainer 3 with an Integrated Music ... biomedical system to aid in rehabilitating individuals with cerebral palsy, traumatic brain injury, stroke ...
Breaking Biology Technology: