Navigation Links
'Super' enzyme may lead way to better tumor vaccines

A "super" form of the enzyme Akt1 could provide the key to boosting the effect of tumor vaccines by extending the lives of dendritic cells, the immune-system master switches that promote the response of T-cells, which attack tumors, said researchers at Baylor College of Medicine in a report that appears in the "advance online publication" section of the current issue of the journal Nature Biotechnology.

"By keeping the dendritic cells alive longer, you extend the window of activation, promoting the desirable immune response, which in the case of cancer, is the expansion of T-cells," said Dr. David Spencer, associate professor of immunology at BCM. "The longer your dendritic cells are alive and active, the more likely you are to expand the appropriate T-helper repertoire and ultimately the desirable cytotoxic (cell killing) T-lymphocytes."

"The dendritic cells are the master switch in the immune system. They decide whether there will be a robust immune response or a tempered immune response to pathogens or cancer," he said.

Using a variety of sophisticated laboratory techniques, Spencer and his colleagues found that Akt1 "was in fact essential for dendritic cell survival," he said. Then they sought to develop a more potent form of Akt1 that would enable the dendritic cells to live longer, boosting immune response.

To do that, they altered the enzyme so that it targeted a particular domain on the plasma membrane of the cell where signaling occurred, making the action of Akt1 more specific. They then eliminated a small part of the Akt1 molecule that had a negative or inhibitory effect.

"It turned out that the altered molecule was much more potent," Spencer said. He credited graduate student Dongsu Park with doing much of the work to develop the super form of Akt1.

Using specially designed adenoviruses, he and his colleagues put the modified "super" Akt1 molecule into the dendritic cells.

"As predicted, these dendritic cells lived longer and were more potent, both in the laboratory and in mice," he said. "It led to the elimination of some very aggressive tumors in the mice."

In the laboratory, they found that the "super" Akt1 also has a potent effect on human dendritic cells as well, although it has not been used to treat people yet.

He expects that when the enzyme is used in people, the first target will be prostate cancer ?a long-standing interest of his laboratory. However, he said, it could be modified to attack other tumors as well.


'"/>

Source:Baylor College of Medicine


Related biology news :

1. Lack of enzyme turns fat cells into fat burners
2. Scientists find missing enzyme for tuberculosis iron scavenging pathway
3. Researchers report new pro-inflammatory role for anti-inflammatory enzyme
4. Purdue researchers use enzyme to clip DNA wires
5. Scientists take aim at virulent bacteria by decoding machinery of key control enzyme
6. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
7. Targeting a key enzyme with gene therapy reversed course of Alzheimers disease in mouse models
8. UCLA researchers identify key enzyme linked to childhood blindness
9. K-State professors discover enzyme responsible for creation of a beetles hard shell
10. Smoking damages key regulatory enzyme in the lung
11. New discovery: If it werent for this enzyme, decomposing pesticide would take millennia
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/13/2017)...  RSA Conference -- RSA, a Dell Technologies business, ... enhance fraud detection and investigation across digital environments ... & Risk Intelligence Suite. The new platform is ... from internal and external sources as well as ... from targeted cybercrime attacks. "Fraudsters are ...
(Date:2/10/2017)... -- Research and Markets has announced the ... Scientific and Commercial Aspects" to their offering. ... Biomarkers play ... therapy for selection of treatment as well for monitoring the ... disease in modern medicine. Biochip/microarray technologies and next generation sequencing ...
(Date:2/8/2017)... YORK , Feb. 8, 2017 About ... individual,s voice to match it against a stored ... such as pitch, cadence, and tone are compared ... require minimal hardware installation, as most PCs already ... for different transactions. Voice recognition biometrics are most ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... RADNOR, Pa., Feb. 24, 2017  VWR Corporation (NASDAQ: VWR), ... to laboratory and production customers, today reported its financial results ... 2016. Highlights: 4Q16 record ... up 1.0% on an organic basis. ... on an organic basis, while the Americas net sales increased ...
(Date:2/23/2017)... ... February 23, 2017 , ... BellBrook Labs is formalizing ... an array of biochemical analyses critical for Lead Discovery. The company’s Lead ... SAR programs, including inhibitor potency and selectivity, mechanism of action, and inhibitor residence ...
(Date:2/23/2017)... Feb. 23, 2017  Seattle,s upscale Capitol Hill neighborhood, with its ... place for a head lice treatment salon to set up ... Tuscan restaurant and a French bistro on E Madison Ave, ... aren,t just any old lice clinic, we pride ourselves on ... and release some of the stigma associated with lice. Everyone ...
(Date:2/23/2017)... ... February 23, 2017 , ... David Nolte, ... Recognition Reception at Purdue Research Park of West Lafayette, Indiana. , ... of outstanding contributions to, and success with, commercializing discoveries from Purdue research. “This ...
Breaking Biology Technology: