Navigation Links
'Super' enzyme may lead way to better tumor vaccines

A "super" form of the enzyme Akt1 could provide the key to boosting the effect of tumor vaccines by extending the lives of dendritic cells, the immune-system master switches that promote the response of T-cells, which attack tumors, said researchers at Baylor College of Medicine in a report that appears in the "advance online publication" section of the current issue of the journal Nature Biotechnology.

"By keeping the dendritic cells alive longer, you extend the window of activation, promoting the desirable immune response, which in the case of cancer, is the expansion of T-cells," said Dr. David Spencer, associate professor of immunology at BCM. "The longer your dendritic cells are alive and active, the more likely you are to expand the appropriate T-helper repertoire and ultimately the desirable cytotoxic (cell killing) T-lymphocytes."

"The dendritic cells are the master switch in the immune system. They decide whether there will be a robust immune response or a tempered immune response to pathogens or cancer," he said.

Using a variety of sophisticated laboratory techniques, Spencer and his colleagues found that Akt1 "was in fact essential for dendritic cell survival," he said. Then they sought to develop a more potent form of Akt1 that would enable the dendritic cells to live longer, boosting immune response.

To do that, they altered the enzyme so that it targeted a particular domain on the plasma membrane of the cell where signaling occurred, making the action of Akt1 more specific. They then eliminated a small part of the Akt1 molecule that had a negative or inhibitory effect.

"It turned out that the altered molecule was much more potent," Spencer said. He credited graduate student Dongsu Park with doing much of the work to develop the super form of Akt1.

Using specially designed adenoviruses, he and his colleagues put the modified "super" Akt1 molecule into the dendritic cells.

"As predicted, these dendritic cells lived longer and were more potent, both in the laboratory and in mice," he said. "It led to the elimination of some very aggressive tumors in the mice."

In the laboratory, they found that the "super" Akt1 also has a potent effect on human dendritic cells as well, although it has not been used to treat people yet.

He expects that when the enzyme is used in people, the first target will be prostate cancer ?a long-standing interest of his laboratory. However, he said, it could be modified to attack other tumors as well.


'"/>

Source:Baylor College of Medicine


Related biology news :

1. Lack of enzyme turns fat cells into fat burners
2. Scientists find missing enzyme for tuberculosis iron scavenging pathway
3. Researchers report new pro-inflammatory role for anti-inflammatory enzyme
4. Purdue researchers use enzyme to clip DNA wires
5. Scientists take aim at virulent bacteria by decoding machinery of key control enzyme
6. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
7. Targeting a key enzyme with gene therapy reversed course of Alzheimers disease in mouse models
8. UCLA researchers identify key enzyme linked to childhood blindness
9. K-State professors discover enzyme responsible for creation of a beetles hard shell
10. Smoking damages key regulatory enzyme in the lung
11. New discovery: If it werent for this enzyme, decomposing pesticide would take millennia
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/23/2017)... , Aug. 23, 2017  The general public,s help is being enlisted ... bacteria that live in and on the human body –and are believed ... The Microbiome Immunity ... human microbiome, starting with the gut. The project's goal is to help ... credit: IBM ...
(Date:6/14/2017)...  IBM (NYSE: IBM ) is introducing several innovative ... to developing collaboration between startups and global businesses, taking place ... the event, nine startups will showcase the solutions they have ... France is one ... a 30 percent increase in the number of startups created ...
(Date:4/24/2017)... , April 24, 2017 ... and partner with  Identity Strategy Partners, LLP (IdSP) ... "With or without President Trump,s March 6, 2017 ... Terrorist Entry , refugee vetting can be instilled with ... resettlement. (Right now, all refugee applications are suspended ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... DIEGO, CALIF. (PRWEB) , ... October 10, 2017 , ... ... as part of its corporate rebranding initiative announced today. The bold new look ... its reach, as the company moves into a significant growth period. , It will ...
(Date:10/10/2017)... firm Parks Associates announced today that Tom Kerber , ... Meeting , October 11 in Scottsdale, Arizona . Kerber ... smart safety and security products impact the competitive landscape. ... Parks Associates: Smart Home Devices: Main Purchase Driver ... "The residential security market has experienced continued growth, and the introduction ...
(Date:10/9/2017)...  BioTech Holdings announced today identification and patenting ... stem cell therapy prevents limb loss in animal ... that treatment with ProCell resulted in more than ... to standard bone marrow stem cell administration.  Interestingly, ... of therapeutic effect.  ...
(Date:10/9/2017)... Texas (PRWEB) , ... October ... ... study published on October 5, 2017, in the medical journal, Epilepsia, Brain ... with the gold standard, video EEG, in detecting generalized tonic-clonic seizures (GTCS) ...
Breaking Biology Technology: