Navigation Links
Study Demonstrates Gene Expression Microarrays are Comparable and Reproducible

sitate that microarray data produced in different laboratories be combined for analysis. Even if all procedures and equipment were the same, small differences between labs, such as in handling the tissue samples, extracting the RNA, or scanning the microarrays, could result in different profiles. To test the feasibility of multi-lab microarray studies, the NCI’s Director’s Challenge program set up this preliminary study to compare results between labs. Twelve different tumor tissues, five cancer cell lines, and five purified RNA samples were prepared, blinded, and randomized for analysis in four laboratories: University of Michigan Medical School, Ann Arbor, Mich.; Dana Farber Cancer Institute/Whitehead Institute, Boston, Mass.; Memorial Sloan-Kettering Cancer Center, New York, N.Y.; and H. Lee Moffitt Cancer Center, Tampa, Fla. All five cell lines and RNA samples were derived from lung carcinomas, while the tissue samples were derived from multiple normal and cancerous tissues. The labs followed a common protocol for all steps of sample preparation and microarray analysis and the resulting gene expression profiles were analyzed and compared. Sample correlation within each lab was extremely high; microarray data from the RNA samples had the highest correlation values, followed by the cell lines, and then finally the tissue samples. This was not surprising, as RNA required the fewest steps of preparation, while tissues required the most. The between-lab correlation values decreased slightly for all samples, but in all cases expression profiles of similar samples could be accurately grouped together. The researchers also examined variations of individual gene measurements to help determine causes of variability, and they found that laboratory practices comprised the smallest source of variation in these studies. Measurement errors common to all labs were the next most common contributor, and biological differences in different samples were the largest source of variat
'"/>

Source:NIH


Page: 1 2 3

Related biology news :

1. Novel Asthma Study Shows Multiple Genetic Input Required; Single-gene Solution Shot Down
2. Emory Study Tests Bone Marrow Stem Cells to Improve Circulation in Legs
3. UCLA Study Shows One-Third of Drug Ads in Medical Journals Do Not Contain References Supporting Medical Claims
4. Study Links Ebola Outbreaks To Animal Carcasses
5. Breakthrough Microarray-based Technology for the Study of Cancer
6. NYU Study Reveals How Brains Immune System Fights Viral Encephalitis
7. Study finds more than one-third of human genome regulated by RNA
8. Leukemia Drug Breakthrough Study In New England Journal Of Medicine
9. Study identifies predictors of HIV drug resistance in patients beginning triple therapy
10. New Study from Affymetrix Laboratories Points to Changing View of How Genome Works
11. Study: Soap And Water Work Best In Ridding Hands Of Disease Viruses
Post Your Comments:
(Date:6/1/2015)... -- According to a new market research ... (Touch-based & Touchless), Application (Consumer Electronics, Automotive, & Others), ... Global Forecast to 2020", published by MarketsandMarkets, the Gesture ... $23.55 Billion by 2020, at a CAGR of 28.23% ... market data T ables and   86   ...
(Date:6/1/2015)... , June 1, 2015 ... "Next Generation Biometrics Market by Application (Government, Military ... Travel & Immigration, and Security), Technology (Face Recognition, ... Signature Recognition, Vein Recognition, and Others), Function (Contact ... , Europe , APAC, ...
(Date:5/29/2015)... , May 28, 2015 Research ... addition of the "Facial Recognition Market by ... - Global Forecast to 2020" report to ... have seen facial recognition technology become more efficient ... to gain traction and investment from the commercial ...
Breaking Biology News(10 mins):Gesture Recognition & Touchless Sensing Market Worth $23.55 Billion by 2020 2Gesture Recognition & Touchless Sensing Market Worth $23.55 Billion by 2020 3Gesture Recognition & Touchless Sensing Market Worth $23.55 Billion by 2020 4Next Generation Biometrics Market Worth $24,448.84 Million by 2020 2Next Generation Biometrics Market Worth $24,448.84 Million by 2020 3Next Generation Biometrics Market Worth $24,448.84 Million by 2020 4Global advanced Facial Recognition Market Expected to Grow From $2.77 Billion in 2015 to $6.19 Billion in 2020, at an Estimated CAGR of 17.4% 2
... topping-out ceremony for the experimental hall of PETRA III ... On occasion of the structural completion of the building, German ... von Beust visited the Helmholtz research centre DESY. Its new ... 2009. PETRA III is a milestone on the way ...
... by Indiana University researchers found the chlorinated flame retardant ... northeastern United States, with by far the highest concentrations ... chemical is produced. The study, by Xinghua Qui ... Public and Environmental Affairs, was published online last week ...
... expected this century. It is likely to intensify droughts, ... migrations and potential conflicts in the areas migrated to. ... Hurricane Katrina in the US, Rafael Reuveny from Indiana ... environmental degradation on population migration, or ecomigration. He examines ...
Cached Biology News:Brilliant perspectives 2Brilliant perspectives 3The proof is in the tree bark 2Environmental exodus 2
(Date:6/1/2015)... Regis Technologies, Inc. is a ... stationary phases (CSPs) for analytical and preparative chromatography ... presenting solutions for new clients, Regis implemented a ... addition to its core expertise in chiral chromatography ... tackle projects for the scale clients need–from milligram ...
(Date:6/1/2015)... 1, 2015  Fennec Pharmaceuticals, Inc. (TSX: FRX, ... results from a poster presented today entitled, "Anti-tumor ... phase III trial of the efficacy of sodium ... cisplatin (Cis) monotherapy for standard risk hepatoblastoma (SR-HB)." ... of Clinical Oncology (ASCO) 2015 Annual Meeting in ...
(Date:6/1/2015)...  Neogen Corporation (NASDAQ: NEOG ) announced today that ... leading commercial food testing laboratory based in southwest ... as a base for the company,s new operations in ... House was incorporated in 1990, and its business has ... water quality testing for major hotels and restaurants in its ...
(Date:6/1/2015)... Frederick MD (PRWEB) June 01, 2015 ... that they have entered into a commercial collaboration ... both companies will combine their technologies and work ... cell (MSC) regenerative technology. RoosterBio and Sistemic will ... and technologies to build more precise and relevant ...
Breaking Biology Technology:Regis Technologies Offers Chiral Chromatography Service 2Regis Technologies Offers Chiral Chromatography Service 3Fennec Announces Presentation Of Positive Interim Sodium Thiosulfate Phase III Safety Data At The 2015 ASCO Annual Meeting 2Fennec Announces Presentation Of Positive Interim Sodium Thiosulfate Phase III Safety Data At The 2015 ASCO Annual Meeting 3Fennec Announces Presentation Of Positive Interim Sodium Thiosulfate Phase III Safety Data At The 2015 ASCO Annual Meeting 4Neogen acquires India food safety company 2Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 2Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 3Sistemic and RoosterBio Inc Announce Broad Collaboration Agreement to Advance miRNA and Mesenchymal Stem/Stromal Cell (MSC) Regenerative Technology 4
... - Oncolytics Biotech Inc. (TSX: ONC, NASDAQ: ... been granted its 33rd U.S. Patent, # 7,608,257, ... With a Virus." The patent claims cover methods ... chemotherapeutic agents to treat patients that are refractory ...
... , COLUMBIA, Md., Oct. 27 Precision ... a component of the National Cancer Institute (NCI),s Clinical ... improving the number and standardizing the quality of monoclonal ... contract award is the result of a third solicitation ...
... Oct. 27 /PRNewswire-Asia/ -- Sinovac Biotech Ltd. (NYSE: ... announced today that,it has received its third purchase order ... and Information Technology for the national,stockpiling plan. Under this ... million doses of PANFLU.1 (15ug/0.5ml) for the Chinese central,government. ...
Cached Biology Technology:Oncolytics(R) Biotech Inc. Announces Issuance of 33rd U.S. Patent 2Precision Antibody Wins Contract to Develop 'Gold Standard' Antibodies as Part of National Cancer Institute's Clinical Proteomics Technologies for Cancer (CPTC) Initiative 2Precision Antibody Wins Contract to Develop 'Gold Standard' Antibodies as Part of National Cancer Institute's Clinical Proteomics Technologies for Cancer (CPTC) Initiative 3Sinovac Obtains Third H1N1 Vaccine Order from Chinese Central Government 2Sinovac Obtains Third H1N1 Vaccine Order from Chinese Central Government 3
Rabbit Anti-Human Secretory Carrier Membrane Protein 2 (SCAMP2 Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: Western Blot...
... Mucin 3 (MUC3) is a glycoprotein. ... a lesser extent in breast, lung and ... shows no cross-reaction with MUC1 and MUC2. ... human MUC3 Immunogen: MUC3 tandem ...
... a member of the caudal-related homeobox family, ... both proliferation and differentiation in intestinal epithelial ... triggering cells towards the phenotype of differentiated ... maintenance of the phenotype. Clone CDX2-88 reacts ...
Rabbit polyclonal to VPAC2 ( Abpromise for all tested applications). entrezGeneID: 7434 SwissProtID: P41587...
Biology Products: