Navigation Links
Stretching DNA to the limit

It has long been known that UV light can damage DNA, reducing its ability to replicate and interact with proteins, and often resulting in the development of skin cancers. However, not much is known about how the elasticity of DNA strands is altered upon exposure to UV light. Now a group of researchers at Duke University have developed a method to measure changes in the mechanical properties of DNA upon irradiation with UV light.

Piotr Marszalek and his colleagues have conducted single-molecule force spectroscopy measurements on viral DNA, which show the unraveling of the DNA double helix upon exposure to UV irradiation. The researchers essentially pick up individual DNA molecules with the tip of a scanning probe microscope and stretch it while measuring the forces generated. These "stretch—release" measurements enable the accurate determination of changes in the elasticity of the DNA strands. Upon exposure to UV light, the force profile of the viral DNA changes dramatically in a dose-dependent manner. The force curve of intact DNA is characterized by a plateau region. This characteristic plateau is drastically reduced in width with increasing exposure to UV light.

UV light induces the crosslinking of the constituent DNA bases within the polynucleotide chains, as well as causes the formation of lesions by linking together the adjacent strands. The small changes in structure induced by this crosslinking can very profoundly affect the ability of DNA to recognize specific molecules, and can thus completely disrupt its ability to replicate and interact with the transcriptional machinery to synthesize proteins. Marszalek and his colleagues have also examined synthetic DNA to figure out the extent to which different bases are affected by UV light. They conclude that the changes in the force profile of viral DNA exposed to UV light are due to the local unwinding of the double helix in some regions arising from the massive formation of crosslinked structures .

"These are the first measurements that establish a relationship between DNA nanomechanics and damage", said Marszalek. He believes that this work paves the way for using stretch—release force spectroscopy measurements in DNA diagnostics.
'"/>

Source:John Wiley & Sons, Inc.


Related biology news :

1. Bumblebees copy one another when contending with unfamiliar flowers
2. New research indicates a troubled greenhouse is brewing
3. DOE JGI launches IMG public online microbial genome data clearinghouse
4. Asleep in the deep: Model helps assess ocean-injection strategy for combating greenhouse effect
5. Food-crop yields in future greenhouse-gas conditions lower than expected
6. Plutonium or greenhouse gases? Weighing the energy options
7. Siberian lakes burp time-bomb greenhouse gas
8. Elephant seal pups suffer from ocean warming
9. Global warming increases oyster sensitivity to pollution
10. Errors in the measurement of global warming corrected
11. Clean skies=faster global warming?

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/19/2016)... 2016 The new GEZE SecuLogic ... web-based "all-in-one" system solution for all door components. It ... the door interface with integration authorization management system, and ... The minimal dimensions of the access control and the ... installations offer considerable freedom of design with regard to ...
(Date:4/14/2016)... April 14, 2016 BioCatch ... Detection, today announced the appointment of Eyal Goldwerger ... role. Goldwerger,s leadership appointment comes at a ... of the deployment of its platform at several of ... technology, which discerns unique cognitive and physiological factors, is ...
(Date:3/31/2016)... , March 31, 2016  Genomics firm Nabsys has ... CEO, Barrett Bready , M.D., who returned to ... the original technical leadership team, including Chief Technology Officer, ... Product Development, Steve Nurnberg and Vice President of Software ... the company. Dr. Bready served as CEO ...
Breaking Biology News(10 mins):
(Date:5/23/2016)... England , May 23, 2016 ... May 25 th at 10:15 a.m. ET before the ... the role genetically engineered mosquitos can play in controlling the ... carrier of the Zika virus.      (Logo: ... engineered male mosquito with a self-limiting gene. Trials in ...
(Date:5/22/2016)... ... ... Doctors in Rome say micronutrients found in certain foods have the potential to ... posted an article on the new research. Click here to read it now. ... Medicine evaluated more than 150 studies on polyphenols in cancer for their report recently ...
(Date:5/20/2016)... MD (PRWEB) , ... May 20, 2016 , ... The ... with Listeria, as reported by Food Safety News on May 12, 2016(1), demonstrates the ... to Ted Olsen, CEO of Baltimore-based biotech firm, PathSensors, Inc. , ...
(Date:5/19/2016)... KS (PRWEB) , ... May 19, 2016 , ... ... contract research organization (CRO) has welcomed Abu Siddiqui as Director, Large Molecule & ... and executing biologics, vaccine and translational biomarker discovery studies for preclinical and clinical ...
Breaking Biology Technology: