Navigation Links
Spelling out cancer on the nanoscale

Tumors start small and stay quiet, yet their intentions are clearly spelled out internally, if we could only read them. No matter how small, every tumor reveals its identity in tiny amounts of abnormally expressed proteins called oncoproteins. Being able to read trace oncoprotein levels from early-stage tumors would speak volumes to physicians and cancer researchers. Enter nano-fluidics and the art of reading a lot from very little.

Researchers led by Dean Felsher at Stanford University School of Medicine, and bioengineers at Cell Biosciences in Palo Alto, collaborated to develop an automated, high-throughput, nano-fluidic system that was able to measure the levels of three oncoproteins: MYC, BCL2, and AKT, in tiny samples drawn as very fine needle aspirates from hematopoetic tumor cells in preclinical transgenic mouse models. The nano-fluidic system physically separates the proteins in very small capillary tubes and then uses antibodies for protein detection. It was also tested on human lymphoma samples.

In previous work, the Felsher group had shown that inactivating MYC induces sustained tumor regression in mice. The researchers decided to test their conditional model of MYC-induced lymphoma by using their nano-fluidic system to quickly measure the impact of targeted protein inhibitors in mice. A series of fine needle-aspirated samples was analyzed, confirming a decrease in oncoprotein levels.

The new nano-fluidic system was also tested on human tumors by measuring the levels of MYC, BCL2, ERK, and AKT proteins in the lymph nodes obtained from patients with Burkitt’s, mantle cell, or follicular lymphoma. The nano-fluidic system reported that MYC was overexpressed in Burkitt’s, while BCL2 was overexpressed in mantle cell and follicular lymphoma patients. In parallel, traditional Western blots were performed to confirm BCL-2 and MYC levels.

"Our strategy can be used to repetitively and quickly assess the levels of oncopro
'"/>

Source:American Society for Cell Biology


Page: 1 2

Related biology news :

1. Viral DNA sequence a possible trigger for breast cancer
2. Enzyme, lost in most mammals, is shown to protect against UV-induced skin cancer
3. Its not all genetic: Common epigenetic problem doubles cancer risk in mice
4. Columbia research lifts major hurdle to gene therapy for cancer
5. Combination therapy boosts effectiveness of telomere-directed cancer cell death
6. Mitochondrial DNA mutations play significant role in prostate cancer
7. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
8. BRCA1 causes ovarian cancer through indirect, biochemical route
9. Researchers identify target for cancer drugs
10. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
11. First atlas of key brain genes could speed research on cancer, neurological diseases
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Spelling out cancer the nanoscale

(Date:8/27/2014)... Currently, the main factors thought to be associated ... injuries are the age of the patient, mechanism ... repair time, repair method, operation technique, and repair ... the repair of peripheral nerve injuries, there is ... good prognosis, and the dose-effect relationship of the ...
(Date:8/27/2014)... Warwick research finds , Gamblers show the same ... research has shown. , Researchers, led by Dr Elliot ... conducted tests that found that both human gamblers and ... than low-value rewards. , Published in Biology Letters ... the important role that memories of previous biggest wins ...
(Date:8/26/2014)... have provided the first evidence that the lack ... early signs of dementia. , Published in ... absence of the protein MK2/3 promotes structural and ... These changes were shown to have a significant ... learning and memory formation capabilities. , An absence ...
Breaking Biology News(10 mins):Gamblers are greedy bird-brains, University of Warwick research finds 2Lack of naturally occuring protein linked to dementia 2
... A current controversy raging in evolutionary biology is ... many genes, each of relatively small effect, or just ... published in Molecular Ecology strongly supports the ... from the Department of Biology and the Redpath Museum, ...
... Robot squirrels from the University of California, Davis, ... a research project on the interaction between squirrels and ... how squirrels signal to snakes with heat and tail ... University and UC Davis aim to learn more about ...
... April 3, 2012 A team led by scientists ... prevent infection from widely differing strains of hepatitis C virus ... very high rate of mutation normally helps it to evade ... to sites on the viral envelope that seldom mutate. One ...
Cached Biology News:Robosquirrels versus rattlesnakes 2Robosquirrels versus rattlesnakes 3Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 2Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 3Scripps Research Institute scientists find promising vaccine targets on hepatitis C virus 4
(Date:8/26/2014)... Aug. 26, 2014 An analysis by Replikins, ... all Dengue virus strains in Pubmed, has revealed extensive ... 1944. This conservation and sharing of specific Replikin gene ... Blocker-Vaccine™ candidate, as it did for the H5N1 influenza ... which have been found to be effective (1,2). ...
(Date:8/26/2014)... Aug. 26, 2014 Technology Applications International ... (NUUU) has two (2) wholly- owned subsidiaries, Renuell ... harness scientifically advanced technology to provide advanced solutions ... Renuell Int,l, Inc. is launching its REJUVEL ... products that is made possible because of its ...
(Date:8/26/2014)... (PRWEB) August 26, 2014 Genedata, ... drug discovery and life science research, today announced ... will be held in Asia, Europe, and ... global pharmaceutical companies and leading research organizations will ... best practices, case studies, and upcoming challenges in ...
(Date:8/26/2014)... , Aug. 26, 2014  Guardant Health, ... commercialization of oncology diagnostic products, has been recognized ... 2015 Technology Pioneer . As an innovator ... of 24 companies across a wide variety of ... Guardant360 ® , Guardant Health,s ...
Breaking Biology Technology:Technology Applications International Corporation, Trading Symbol NUUU now trading on NASDAQ's Over the Counter Bulletin Board (OTC BB) 2Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 2Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 3Guardant Health Named a 2015 Technology Pioneer by World Economic Forum 2
... QED International Associates, Inc.,administrator for the ... fund portfolios, today announced that effective at ... 2008, Alexion Pharmaceutical, Inc.,(Nasdaq: ALXN ) ... ASPV ) in,the HealthShares(TM) Autoimmune-Inflammation Index. Alpharma, ...
... ARLINGTON, Va., Jan. 4 Faced with ... suppliers are turning their attention to,opportunities in ... navigate these unfamiliar waters, leading research and,advisory ... report,examining the marketing and media preferences of ...
... Md., Jan. 4 Human Genome Sciences,Inc. (Nasdaq: ... 26th,Annual JPMorgan Healthcare Conference will be webcast and may ... ), A member of Human Genome Sciences, ... on Wednesday, January 9, 2008 at 7:30 am Pacific,Time, ...
Cached Biology Technology:QED International Associates Announces Changes to the HealthShares(TM) Autoimmune-Inflammation and Neuroscience Indexes - Correction 2Study Examines Media Preferences of Life Scientists in Applied Markets 2Study Examines Media Preferences of Life Scientists in Applied Markets 3
... The CopyControl™ vector can be induced prior ... vectors provide the stability of single-copy BACs ... or other cloning vectors. The kits provide ... reagents, insert size screening system) necessary to ...
ID clarifier: Without ethidium bromide...
... Acridine orange has been used as a ... and polyacrylamide gels. Acridine orange at a ... DNA in gels with a sensitivity of ... been used extensively for cell staining of ...
... rich in GC content or with significant ... amplify, and when subjected to PCR result ... and consistency can be increased by the ... reactions. Polymer-Aide is an effective PCR enhancer ...
Biology Products: