Navigation Links
Spelling out cancer on the nanoscale

Tumors start small and stay quiet, yet their intentions are clearly spelled out internally, if we could only read them. No matter how small, every tumor reveals its identity in tiny amounts of abnormally expressed proteins called oncoproteins. Being able to read trace oncoprotein levels from early-stage tumors would speak volumes to physicians and cancer researchers. Enter nano-fluidics and the art of reading a lot from very little.

Researchers led by Dean Felsher at Stanford University School of Medicine, and bioengineers at Cell Biosciences in Palo Alto, collaborated to develop an automated, high-throughput, nano-fluidic system that was able to measure the levels of three oncoproteins: MYC, BCL2, and AKT, in tiny samples drawn as very fine needle aspirates from hematopoetic tumor cells in preclinical transgenic mouse models. The nano-fluidic system physically separates the proteins in very small capillary tubes and then uses antibodies for protein detection. It was also tested on human lymphoma samples.

In previous work, the Felsher group had shown that inactivating MYC induces sustained tumor regression in mice. The researchers decided to test their conditional model of MYC-induced lymphoma by using their nano-fluidic system to quickly measure the impact of targeted protein inhibitors in mice. A series of fine needle-aspirated samples was analyzed, confirming a decrease in oncoprotein levels.

The new nano-fluidic system was also tested on human tumors by measuring the levels of MYC, BCL2, ERK, and AKT proteins in the lymph nodes obtained from patients with Burkitt’s, mantle cell, or follicular lymphoma. The nano-fluidic system reported that MYC was overexpressed in Burkitt’s, while BCL2 was overexpressed in mantle cell and follicular lymphoma patients. In parallel, traditional Western blots were performed to confirm BCL-2 and MYC levels.

"Our strategy can be used to repetitively and quickly assess the levels of oncopro teins in cancer cells grown in the laboratory and in human patients," says Felsher. "It may prove useful for the early detection of cancer, and for monitoring patients?responses during their treatment, allowing clinicians to tailor treatments to individual patients. Finally, we provide a high throughput method to identify if new cancer drugs are effective at targeting specific oncoproteins."
'"/>

Source:American Society for Cell Biology


Related biology news :

1. Viral DNA sequence a possible trigger for breast cancer
2. Enzyme, lost in most mammals, is shown to protect against UV-induced skin cancer
3. Its not all genetic: Common epigenetic problem doubles cancer risk in mice
4. Columbia research lifts major hurdle to gene therapy for cancer
5. Combination therapy boosts effectiveness of telomere-directed cancer cell death
6. Mitochondrial DNA mutations play significant role in prostate cancer
7. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
8. BRCA1 causes ovarian cancer through indirect, biochemical route
9. Researchers identify target for cancer drugs
10. Weizmann Institute scientists develop a new approach for directing treatment to metastasized prostate cancer in the bones.
11. First atlas of key brain genes could speed research on cancer, neurological diseases

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/17/2017)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... its 2016 Annual Report on Form 10-K on Thursday April 13, ... ... the Investor Relations section of the Company,s website at http://www.nxt-id.com ... http://www.sec.gov . 2016 Year Highlights: ...
(Date:4/11/2017)... April 11, 2017 No two people ... at the New York University Tandon School of ... have found that partial similarities between prints are ... in mobile phones and other electronic devices can ... The vulnerability lies in the fact that fingerprint-based ...
Breaking Biology News(10 mins):
(Date:10/9/2017)... ... October 09, 2017 , ... The award-winning American Farmer television ... quarter 2018. American Farmer airs Tuesdays at 8:30aET on RFD-TV. , With global ... the challenge of how to continue to feed a growing nation. At the same ...
(Date:10/9/2017)... ... October 09, 2017 , ... ... October 5, 2017, in the medical journal, Epilepsia, Brain Sentinel’s SPEAC® System ... standard, video EEG, in detecting generalized tonic-clonic seizures (GTCS) using surface electromyography ...
(Date:10/7/2017)... 6, 2017  The 2017 Nobel Prize in ... Jacques Dubochet, Joachim Frank and ... cryo-electron microscopy (cryo-EM) have helped to broaden ... biology community. The winners worked with systems manufactured ... produce highly resolved, three-dimensional images of protein structures ...
(Date:10/5/2017)... , ... October 05, 2017 , ... LabRoots , ... scientists from around the world, is giving back to cancer research with a month-long ... , Now through October 31, shoppers can use promo code PinkRibbon to get ...
Breaking Biology Technology: