Navigation Links
'Smart' nanoprobes light up disease

Researchers from Rice University's Center for Biological and Environmental Nanotechnology (CBEN) have developed a "smart" beacon hundreds of times smaller than a human cell that is programmed to light up only when activated by specific proteases. Altered expression of particular proteases is a common hallmark of cancer, atherosclerosis, and many other diseases.

In the September issue of the journal Biochemical and Biophysical Research Communications, lead authors Jennifer West, the Isabel C. Cameron Professor of Bioengineering and director of CBEN's biological research program, and Rebekah Drezek, the Stanley C. Moore Assistant Professor of Bioengineering and assistant professor of electrical and computer engineering, describe development of a new nanoprobe for visualization of proteolytic activity in vivo.

"The idea is to develop a 'smart' nanostructure that is dark in its original state but lights up very brightly in the presence of enzymatic activity associated with a particular disease process," said West. "Other groups have used targeted nanostructures including quantum dots for molecular imaging, but they have never been able to adequately solve the problem of clearly distinguishing between the 'cancer is here' signal and the background light which arises from nanostructures not specifically bound to their molecular targets."

Rice's technology solves this longstanding problem by using emissive nanoparticles called quantum dots that give off light in the near-infrared (NIR), a rare portion of the spectrum that has no background component in biomedical imaging. Near-infrared light also passes harmlessly through skin, muscle and cartilage, so the new probes could alert doctors to tumors and other diseases sites deep in the body without the need for a biopsy or invasive surgery.

The probe's design makes use of a technique called "quenching" that involves tethering a gold nanoparticle to the quantum dot to inhibit luminescence. The te ther, a peptide sequence measuring only a few nanometers, or billionths of a meter, holds the gold close enough to prevent the quantum dot from giving off its light.

In their test system, the Rice team used a peptide tether that is cleaved by the enzyme collagenase. The researchers first showed that luminescence of the quantum dots was cut by more than 70 percent when they were attached to the gold particles. They remained dark until the nanostructures were exposed to collagenase after which the luminescence steadily returned.

Ultimately, the researchers hope to pair a series of quantum dots, each with a unique NIR optical signature, to an index of linker proteases.

"There is currently a critical need for methods to simultaneously image the activity of multiple proteases in vivo," said Drezek. "This is important not only for early detection of several diseases, but perhaps more significantly, in understanding and monitoring the efficacy of therapeutic interventions, including the growing class of drugs that act as protease inhibitors. What is particularly powerful about the protease imaging probes described in this study is the combination of the contrast enhancement achievable through an activateable probe with the imaging advantages provided by the brightness, photostability, and tunability of quantum dots."


'"/>

Source:Rice University


Related biology news :

1. Smart genetic therapy helps the body to heal itself
2. UC Davis researchers use heated nanoprobes to destroy breast cancer cells in mice
3. Researchers discover way to make cells in the eye sensitive to light
4. Recent breakthroughs in common adult leukemia highlighted in New England Journal of Medicine
5. Bacteria collection sheds light on urinary tract infections
6. Sea skate experiment sheds light on human cell transport
7. X-Ray Beams And Fruit Fly Flight Simulator Aid Scientists View Of Muscle Power
8. McGill researchers shed light on formation of carcinogen in food
9. Scientists discover how plants disarm the toxic effects of excessive sunlight
10. Medical molecules designed to respond to visible light that can penetrate tissue
11. Genetic defects give the immune system the green light to attack the pancreas
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/23/2017)... 2017  Hunova, the first robotic gym for the rehabilitation and functional ... in Genoa, Italy . The first 30 robots will ... USA . The technology was developed and patented at ... IIT spin-off Movendo Technology thanks to a 10 million euro investment from ... click: ...
(Date:5/16/2017)... 2017   Bridge Patient Portal , an ... MD EMR Systems , an electronic medical record ... have established a partnership to build an interface ... GE Centricity™ products, including Centricity Practice Solution (CPS), ... These new integrations will allow healthcare delivery networks ...
(Date:4/24/2017)... Janice Kephart , former 9/11 ... Partners, LLP (IdSP) , today issues the following ... March 6, 2017 Executive Order: Protecting the ... be instilled with greater confidence, enabling the reactivation ... applications are suspended by until at least July ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... , ... October 11, 2017 , ... ... announced today it will be hosting a Webinar titled, “Pathology is going digital. ... Associates , on digital pathology adoption best practices and how Proscia improves lab ...
(Date:10/11/2017)... ... ... Disappearing forests and increased emissions are the main causes of the evolving ... those living in larger cities are affected by air pollution related diseases. , That ... countries globally - decided to take action. , “I knew I had to take ...
(Date:10/10/2017)... ... October 10, 2017 , ... San Diego-based team building and cooking events ... announced today. The bold new look is part of a transformation to increase ... a significant growth period. , It will also expand its service offering from its ...
(Date:10/10/2017)... (PRWEB) , ... October 10, 2017 , ... Dr. Bob ... at his local San Diego Rotary Club. The event entitled “Stem ... CA and had 300+ attendees. Dr. Harman, DVM, MPVM was joined by two ...
Breaking Biology Technology: