Navigation Links
Silver bullet: UGA researchers use laser, nanotechnology to rapidly detect viruses

Waiting a day or more to get lab results back from the doctor's office soon could become a thing of a past. Using nanotechnology, a team of University of Georgia researchers has developed a diagnostic test that can detect viruses as diverse as influenza, HIV and RSV in 60 seconds or less.

In addition to saving time, the technique ?which is detailed in the November issue of the journal Nano Letters ?could save lives by rapidly detecting a naturally occurring disease outbreak or bioterrorism attack.

"It saves days to weeks," said lead author Ralph Tripp, Georgia Research Alliance Eminent Scholar in Vaccine Development at the UGA College of Veterinary Medicine. "You could actually apply it to a person walking off a plane and know if they're infected."

The technique, called surface enhanced Raman spectroscopy (SERS), works by measuring the change in frequency of a near-infrared laser as it scatters off viral DNA or RNA. This change in frequency, named the Raman shift for the scientist who discovered it in 1928, is as distinct as a fingerprint.

This phenomenon is well known, but Tripp explained that previous attempts to use Raman spectroscopy to diagnose viruses failed because the signal produced is inherently weak.

But UGA physics professor Yiping Zhao and UGA chemistry professor Richard Dluhy experimented with several different metals and methods and found a way to significantly amplify the signal. Using a method they've patented, they place rows of silver nanorods 10,000 times finer than the width of a human hair on the glass slides that hold the sample. And, like someone positioning a TV antenna to get the best reception, they tried several angles until they found that the signal is best amplified when the nanorods are arranged at an 86-degree angle.

"The enhancement factors are extraordinary," Dluhy said. "And the nice thing about this fabrication methodology is that it's very easy to implement, it's very che ap and it's very reproducible."

Tripp said the technique is so powerful that it has the potential to detect a single virus particle and can also discern virus subtypes and those with mutations such as gene insertions and deletions. This specificity makes it valuable as a diagnostic tool, but also as a means for epidemiologists to track where viruses originate from and how they change as they move through populations.

The researchers have shown that the technique works with viruses isolated from infected cells grown in a lab, and the next step is to study its use in biological samples such as blood, feces or nasal swabs. Tripp said preliminary results are so promising that the researchers are currently working to create an online encyclopedia of Raman shift values. With that information, a technician could readily reference a Raman shift for a particular virus to identify an unknown virus.

To make their finding commercially viable, they're developing a business model, seeking venture capital and exploring ways to mass produce the silver nanorods. Next year, they plan on moving their enterprise to the Georgia BioBusiness Center, an UGA incubator for startup bio-science companies.

Presently, viruses are first diagnosed with methods that detect the antibodies a person produces in response to an infection. Tripp explained that these tests are prone to false positives because a person can still have antibodies in their system from a related infection decades ago. The tests are also prone to false negatives because some people don't produce high levels of antibodies.

Because of these limitations, antibody based tests often must be confirmed with a test known as polymerase chain reaction (PCR), which detects the virus itself by copying it many times. The test can take anywhere from several days to two weeks. Tripp said the latter is clearly too long, especially in light of emerging threats such as H5N1 avian influenza.

"For some respiratory viruses, you've either cleared the infection at that point or succumbed to the infection," Tripp said. "What we've developed is the next generation of diagnostic testing."


'"/>

Source:University of Georgia


Related biology news :

1. NYU researchers simulate molecular biological clock
2. Vital step in cellular migration described by UCSD medical researchers
3. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
4. UCSD researchers maintain stem cells without contaminated animal feeder layers
5. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
6. New protein discovered by Hebrew University researchers
7. First real-time view of developing neurons reveals surprises, say Stanford researchers
8. Agilent Technologies releases automated literature search tool for biology researchers
9. Self-assembled nano-sized probes allow Penn researchers to see tumors through flesh and skin
10. Yale researchers identify molecule for detecting parasitic infection in humans
11. US life expectancy about to decline, researchers say
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/13/2017)... , April 13, 2017 According to a new ... Authentication, Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, ... IAM Market is expected to grow from USD 14.30 Billion in 2017 ... (CAGR) of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/11/2017)... 2017 NXT-ID, Inc. (NASDAQ:   NXTD ... the appointment of independent Directors Mr. Robin D. Richards ... of Directors, furthering the company,s corporate governance and expertise. ... Gino Pereira , Chief ... to their guidance and benefiting from their considerable expertise as ...
(Date:4/5/2017)... KEY FINDINGS The global market ... CAGR of 25.76% during the forecast period of 2017-2025. ... for the growth of the stem cell market. ... MARKET INSIGHTS The global stem cell market is segmented ... The stem cell market of the product is segmented ...
Breaking Biology News(10 mins):
(Date:4/27/2017)... ... 27, 2017 , ... Arrowhead Publishers is pleased to announce ... to San Diego, CA on September 27-28, 2017. Leaders from the pharmaceutical, biotech, ... in the treatment of various types of pain. There are also extended networking ...
(Date:4/27/2017)... (PRWEB) , ... April 27, 2017 , ... ... the 2017 Borlaug CAST Communication Award goes to Jayson Lusk, a consummate communicator ... forms of media to advocate for science, as he explains how innovation and ...
(Date:4/26/2017)... ... April 26, 2017 , ... Led by ... I clinical trials comes to Tampa, San Francisco and Boston in 2017. ... FDA regulated organizations such as Pfizer Inc., Teva Pharmaceuticals, Advaxis, Inc., Ocular Therapeutix ...
(Date:4/26/2017)... 26, 2017  Genisphere LLC, provider of the ... a collaborative and sponsored research agreement with the ... . The overall goal of the partnership is ... 3DNA designs and formulations after in vivo ... the vasculature as well as inflammatory responses, demonstrating ...
Breaking Biology Technology: