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Sequencing of the oyster mushroom genome

would be equivalent to a volume of more than 11,500 pages of text. If the pages are normal, folio size and are placed side by side, they would run for a distance of 3.5 kilometres; the letters thereof, written and placed one after the other, would run to 141 km. The genome of the mushroom has twin sets of the genes and, thereby, each set has about 6,000 "pages" on which we estimate there are some 12,000 genes - approximately two genes per page. Thus, the real task now is to determine where each of these genes starts and finishes, what they do and how they do it.

Order 70 million letters

With the selection of the project by the Genomics Institute, it will be this United States-based body that will be responsible for carrying out the sequencing work and computer analysis.

At the Public University of Navarre laboratories the DNA of the oyster mushroom will be isolated and purified and then sent to the Genomics Institute for sequencing. Within one year, approximately, the JGI will have undertaken a first reading of the genome's 70 million letters. And, once again, it will be laboratories at the Public University of Navarre that will order the sequenced fragments and co-ordinate the rest of the project tasks.

From the Navarre university, the resulting computer archive of the sequencing, containing the definitive "pages of letters" for the genetic code, will be then distributed to the other participating laboratories in order to carry out the annotation of the genome sequence ?involving the identification of each one of the genes that make up the oyster mushroom, i.e. the genetic constitution of the organism.

Life appeared on Earth some 3,000 million years ago. The evolution undergone by this fungus whose DNA is to be purified, has been through these 3,000 million years, as has the human being. Thus, within its genome there is 3,000 million years of history ?the goal of the sequencing of this genome is to unlock and
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Source:Elhuyar Fundazioa


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