Navigation Links
Sea coral's trick helps scientists tag proteins

ed the DNA sequence that encodes the fluorescent protein, then expressed the resulting mutant proteins in bacteria until they found one that could be irreversibly converted from green to red.

As with GFP, the sequence for Dendra and an accompanying short amino acid tail can be inserted into the gene encoding the protein a scientist wants to study. In living cells, the fluorescent tail is produced along with the protein, acting like a beacon to researchers who want to learn what the main protein does and where it goes.

To test how well the new tag labeled and tracked target proteins, the team fused Dendra to proteins that form the cytoskeleton of the cell, including actin and tubulin filaments and found the expected patterns of intracellular protein distribution.

Dendra belongs to a small family of green-to-red PAFPs. The first protein in this family, Kaede--which is Japanese for maple leaf--was discovered by Japanese researcher Atsushi Miyawaki four years ago. "All known Kaede-like proteins are sensitive to ultraviolet light irradiation, which results in immediate conversion from green to red fluorescence, but they are not sensitive to blue light," Konstantin Lukyanov said.

So the researchers did not expect Dendra to be activated by low-toxic visible light. "It was very surprising for us to observe an efficient Dendra photoconversion upon illumination with intense blue light," Konstantin Lukyanov said. "We still have no clear explanation for why Dendra differs from other Kaede-like fluorescent proteins."

Sergey Lukyanov pointed out that the ultraviolet light required to activate many PAFPs can be toxic to cells, and can dramatically alter their biochemistry. Further, the equipment needed to generate ultraviolet light can be expensive and in short supply. "We anticipate that Dendra will broaden considerably the circle of PAFP users," he said.


'"/>

Source:Howard Hughes Medical Institute


Page: 1 2 3

Related biology news :

1. Old drug, new tricks: Prospects for slashing the impact of malaria
2. Dangerous tricksters: Some bacterie use immune cells to reproduce
3. Researchers use trickery to create immune response against melanoma
4. New finding points way to foiling anthraxs tricks
5. A wolf in sheeps clothing: Plague bacteria reveal one of their virulence tricks
6. An AIDS-related virus tricks cells to become tumors, new Penn study finds
7. Scientists perform tricky operation
8. Yale biologists trick viruses into extinction
9. Toxoplasmosis infection trick revealed by scientists
10. Jumping gene helps explain immune systems abilities
11. Protein helps regulate the genes of embryonic stem cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Sea coral trick helps scientists tag proteins

(Date:12/15/2014)... , Dec. 12, 2014 Research and Markets ... the "Global Facial Recognition Market 2015-2019" report ... Facial recognition is a technology used ... the overall facial feature of a person such as ...
(Date:12/11/2014)... 10, 2014  Data Sciences International (DSI), the global ... new series of digital telemetry implants to meet ... series, part of the PhysioTel™ Digital platform, was ... physiologic data when incorporating functional endpoints into repeat-dose ...
(Date:12/10/2014)... 9, 2014 CIE San Diego has launched an innovative ... that enhances care coordination among social service and care providers ... second $1 million grant from the Alliance Healthcare Foundation to ... be recognized as a "Live Well San Diego" partner on ...
Breaking Biology News(10 mins):Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3New telemetry implants expected to change how large animal toxicology studies are conducted 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4
... in on finding an effective bait to get ahead ... is now killing not only lodgepole pine forests, but ... Research Chair in Forest Entomology in the University of ... emitted by the pest in North America,s lodgepole and ...
... multi-model-based assessment of so-called Durban Platform scenarios, conducted by ... Institute for Climate Impact Research (PIK) and the Fondazione ... is the current negotiation track at the Warsaw climate ... by 2015 to come into effect in 2020. ...
... new research shows that the Plasmodium vivax ... in 2010, may be "rapidly evolving" to overcome the natural ... Africans, scientists reported today at the annual meeting of the ... In large swaths of sub-Saharan Africa, some 95 ...
Cached Biology News:Bait research focused on outsmarting destructive beetle 2Success of climate talks vital for 2°C target 2Success of climate talks vital for 2°C target 3New research finds potential risk for millions in Africa believed resistant to vivax malaria 2New research finds potential risk for millions in Africa believed resistant to vivax malaria 3New research finds potential risk for millions in Africa believed resistant to vivax malaria 4
(Date:1/22/2015)... (PRWEB) January 22, 2015 Crystal Diagnostics (CDx) ... that it has received AOAC-PTM Certifications for the six non-O157 ... and O145; collectively referred to as STEC or the “Big-6”) ... colony forming unit (cfu) per 325 g of raw ground ...
(Date:1/22/2015)... 22, 2015 Dr. Greg Leyer of UAS ... Scripps Natural Supplements Pre-Conference seminar on probiotics in San Diego, ... annual continuing education conference for health care professionals. This year’s ... topic of probiotics in health. Dr. Leyer spoke about the ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Diagenode, ... such as the Bioruptor® and complete solutions for ... for chromatin immunoprecipitation, alleviating the need for manual ... controls needed for ChIP of histones or transcription ...
(Date:1/22/2015)... 22. 2015  Varian Medical Systems (NYSE: VAR ), ... for its commitment to sustainability with inclusion on a prestigious ... highest ranked healthcare equipment company among the Corporate Knights Global ... Economic Forum at Davos, Switzerland . ...
Breaking Biology Technology:Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2Diagenode Launches Unique Semi-Automated Solution for Chromatin Immunoprecipitation 2Varian Honored Among World's 100 Most Sustainable Corporations 2
... In a move designed to give Wisconsin a competitive ... a partnership agreement with the Wisconsin Alumni Research ... research at non-profit and academic institutions in Wisconsin a ... held by WARF. , ,In what the administration called ...
... in the first televised debate in Wisconsin's campaign for governor ... asked Gov. Jim Doyle and challenger Mark ... Beene, who had worked for Pabst Brewery before its 1997 ... closing all over the place" - and he wanted to ...
... Wis. - The Center for International Blood and ... Wisconsin has won a contract worth $6,180,029 to collect ... cell transplants that use cells from bone marrow or cord ... part of the U.S. Department of Health and Human ...
Cached Biology Technology:Doyle, WARF announce partnership to lure stem cell companies 2Doyle, WARF announce partnership to lure stem cell companies 3Candidates' sparring over jobs focuses debate on right issue 2Candidates' sparring over jobs focuses debate on right issue 3Medical College to build $6M transplant database 2
... Kinase (PNK) catalyzes the transfer of ... 5'-hydroxyl of single- and double-stranded DNA, ... also removes the 3'-phosphate from 3'-phosphoryl ... 5'-diphosphates to form a 3'-hydroxyl group. ...
MOUSE ANTI GTP BINDING PROTEIN...
We offer solid and solution phase peptide synthesis including a whole range of modifications. Please send the details of your peptide synthesis need to info@rpeptide.com...
Maxi XL Hybridization Oven for Extra Large bottles. 6 bottle (70 mm) rotisserie + shaker tray available for simultaneous use. Includes linear and orbital shaker trays, 4 extra large bottles, pack o...
Biology Products: