This discovery takes scientists another step closer to being able to grow embryonic stem cells without the “feeder layer?of mouse fibroblast cells that is essential for maintaining the pluripotency of embryonic stem cells, says the study’s primary investigator, Michael Kahn, Ph.D., who was recently named the first Provost’s Professor of Medicine and Pharmacy at USC. Such a layer is needed because it is currently the only proven method to provide the stem cells with the necessary chemical signals that prompt them to stay undifferentiated and to continue dividing over and over.
Still, growing human embryonic stem cells on a layer of mouse fibroblasts has never made much sense to the scientists forced to do just that. “Stem cells that grow on feeders are contaminated with mouse glycoproteins markers,?Kahn says. “If you use them into humans, you’d potentially have a horrible immune response.?
And so, in order to take any eventual stem cell-based treatments from the laboratory to the clinic, there needs to be a way to keep the cells growing and dividing without the use of mouse fibroblasts. The discovery of IQ-1, says Kahn, is a significant step in that direction.
What IQ-1 does, Kahn explains, is to block one arm of a cell-signaling pathway called the Wnt pathway, while enhancing the signal coming from the other arm of the Wnt pathway. The Wnt pathway is known to have dichotomous effects on stem cells i.e. bot
Source:University of Southern California