Navigation Links
Scientists rid stem cell culture of key animal cells

natomy and a co-author of the Nature Methods paper. "This development is a good step in that direction. Also, clinically, the feeder cells were one of the main sources of potential contamination with pathogens, so their elimination should improve safety. However, not all the animal components have been removed from the media yet, but this is an important step."

Working with three of WiCell's five human embryonic stem cell lines, Xu and his team explored the molecular interactions within the stem cell growth medium. He discovered that, in certain conditions, a protein known as fibroblast growth factor 2 (FGF2) accomplishes the same critical role that feeder cells are thought to play: ensuring that the stem cells remain in their undifferentiated state and capable of proliferation.

"We've got it down to the mechanism," Xu says.

Moreover, Xu made the surprising discovery that the very molecules that encourage human embryonic stem cells to differentiate appear to inhibit differentiation in mouse embryonic stem cells.

Aside from feeder cells, two other sources of animal material remain in stem cell culture materials. One, Matrigel, is a product that is essentially a plate-coating matrix of cells extracted from mouse tumors. Serum replacement, which is bovine in origin, is the other animal material still needed to culture stem cells.

Xu became interested in unveiling molecules derived from the mouse feeder cells because, in their absence, stem cells start to differentiate within two to three days. Xu started by evaluating the effect of changing stem cell growth conditions - using less feeder cell material, or no serum replacement, for instance.

Unexpectedly, Xu found that the presence of serum replacement promoted stem cell differentiation. Digging deeper, he found that serum replacement mimics the activity of bone morphogenetic protein (BMP), a molecule known to kick-start embryonic development, or in this case, cell differe
'"/>

Source:University of Wisconsin-Madison


Page: 1 2 3

Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists develop new color-coded test for protein folding
11. Scientists identify genetic pathways essential to RNA interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Scientists rid stem cell culture key animal cells

(Date:11/21/2014)... , Nov. 19, 2014  Earlier this year ... New York College, and one of the most prolific ... signals that are transmitted from Smartphones to third party ... Smartphones and has one of the earliest known patents ... its usage in the military, child care, elder care ...
(Date:11/21/2014)... , Nov. 20, 2014   Atmel® Corporation ... microcontroller (MCU) and touch technology solutions, today launched the ... with the widest V cc range from ... accuracy and faster I 2 C bus communication speeds, ... EEPROM memory making them ideal for consumer, industrial, computer, ...
(Date:11/18/2014)... The Parenteral Drug Association (PDA) today confirmed that ... and at least seven more will participate in the upcoming ... Hotel in Washington D.C. , Dec. 2-4. ... support from the regulatory agencies in the United ... our effort to help advance the use of metrics in ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... As 17 countries and 30 organizations launch ... major environmental groups World Wildlife Fund, The ... governments, businesses, scientists, non-governmental organizations and individuals around ... coral reefs. The International Year of the Reef ...
... Stowers Institutes Proteomics Center has published a novel method ... of affinity purifications analyzed by mass spectrometry (APMS) to ... to associate with one another. The ... not only to the interactions within well-defined protein assemblies, ...
... as many scientists had given up the search, researchers ... cells with the capacity to generate new insulin-producing beta ... for humans, the newfound progenitor cells will represent an ... diabetes, the researchers report in the Jan. 25 issue ...
Cached Biology News:Environmental groups call for increased protection of coral reefs 2Stowers Proteomics Center devises method for assigning probabilities to human protein interactions 2Elusive pancreatic stem cells found in adult mice 2
(Date:12/24/2014)... On Friday, December 19, 2014, President ... and Continuing Resolution Appropriations Act of 2015, which ... eligible to receive funding through the Congressionally Directed ... of Defense (DoD). The Hydrocephalus Association (HA), working ... is celebrating this victory for the over 1 ...
(Date:12/24/2014)... 2014 The report expects global cell ... 2019. It also provides a carefully analyzed data about ... for the market. , Full Copy of Report @ ... global market for cell expansion will keep witnessing growth ... this growth to be driven by rapid technological advancements, ...
(Date:12/24/2014)... -- PharmaBoardroom,s new report, , Healthcare ... for free download , digs deep into this vibrant ... the sector today. One area where ... developing a homegrown pharmaceutical manufacturing base, even if the government,s ... way off. A cursory comparison with neighboring Morocco ...
(Date:12/22/2014)... The Center for Professional Innovation ... training across the life sciences industry, has partnered with ... to provide the organization's members with discounted classroom courses, ... the more than 350 sessions across 80 course titles. ... when registering for a public course at ...
Breaking Biology Technology:Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3
... Truman Medical Centers (TMC),became one of the first hospitals ... AS 128 slice CT scanner this week. The 128-slice,CT ... with minimal radiation exposure to the patient., "To ... country to have this,truly, first of its kind, state-of-the-art ...
... - Enerkem Inc., a leading second,generation biofuels ... new Vice-Presidents to its executive team: Mr. ... Marie-Helene Labrie,Vice-President, Government Affairs and Communications., ... executives to our management,team," said Mr. Vincent ...
... Aug. 28 deCODE genetics,(Nasdaq: DCGN ) today ... deCODE,CSO Jeffrey Gulcher,s presentation at Thomas Weisel Partners Healthcare,Conference ... PM ET / 5:30 PM GMT / 6:30,PM British ... Seasons Hotel,Boston, MA. The webcast can be accessed through ...
Cached Biology Technology:Truman Medical Centers Becomes One of the First Hospitals in the U.S. To Install the World's First Adaptive 128-slice CT Scanner 2Enerkem Expands Executive Management Team 2deCODE genetics to Webcast Presentation at Thomas Weisel Partners Healthcare Conference 2008 2
Sf21 Insect Cells (Frozen) in Max-XP Medium...
... is used for conventional PCR and thermal ... personal thermal cycler, which features a 96-well ... tubes or plates, programmable time, temperature, and ... modes of temperature control. The system also ...
... ChipWriter Pro system with colony-picking capabilities, 110-120 ... array DNA, proteins, oligonucleotides, carbohydrates, lipids, and ... for microarray applications. This system can also ... as 300 micrometers in diameter and for ...
... Ciphergen's ProteinChip arrays have been developed ... biological samples. A variety of ProteinChip ... proteins according to their unique biochemical ... spots, allowing side-by-side sample comparison, and ...
Biology Products: