Navigation Links
Scientists rid stem cell culture of key animal cells

Tackling a pressing and controversial technical barrier in stem cell biology, scientists at the WiCell Research Institute and the University of Wisconsin-Madison have crafted a recipe that allows researchers to grow human embryonic stem cells in the absence of mouse-derived "feeder" cells, long thought to be a source of potential contamination for the therapeutically promising cells.

The new findings, appear today (Feb. 17) in the journal Nature Methods and come on the heels of a recent University of California study showing that existing stem cell lines are already contaminated with an animal molecule. The potential threat of animal pathogens tainting human stem cell lines poses a problem for the safe clinical use of many, if not all, of the current cell lines now in use.

Until now, scientists have had to grow and sustain stem cells through the tedious daily task of generating mouse feeder cells from mouse embryos. Feeder cells, or fibroblasts, are connective tissue cells that form the matrix upon which stem cells grow.

The mouse feeder cells were an important ingredient in the mix of culture materials required to keep stem cells in their undifferentiated "blank slate" state. Embryonic stem cells are capable of forming any of the 220 tissues and cells in the human body and, in culture, are constantly trying to migrate down different developmental pathways. Maintaining stock cultures in their undifferentiated state is critical.

The feeder cell dogma now can be overturned, says lead investigator Ren-He Xu, a senior scientist at WiCell, a private, nonprofit research institute. "This work completely gets rid of the need for feeder cells," says Xu. "It also significantly reduces the daily labor of preparing the feeder cell-conditioned medium."

"It is important that the culture of human ES cells be simplified so that the average scientist can use them without extensive prior training," says James Thomson, a UW-Madison professor of a
'"/>

Source:University of Wisconsin-Madison


Page: 1 2 3

Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists develop new color-coded test for protein folding
11. Scientists identify genetic pathways essential to RNA interference
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Scientists rid stem cell culture key animal cells

(Date:7/24/2014)... researchers and patients have hoped that embryonic stem cells ... bodycould provide insight into numerous diseases perhaps even be ... by the inability to transfer research and tools from ... because human ESCs are "primed" and slightly less plastic ... Powell, and Haoyi Wang, who are scientists in the ...
(Date:7/24/2014)... Africa in mid-July 2014, as the annual fire season ... areas of increased temperatures, are heavily sprinkled across the ... Congo (northeast), and Zambia (southeast). Thick gray smoke rises ... especially in the Democratic Republic of the Congo, strong ... fire season is an annual event in this region, ...
(Date:7/24/2014)... release is available in German . ... to recognize and respond to threats to health, and ... detailed understanding of the mechanism underlying immunologic memory, however, ... have converged to support the hypothesis that the persistence ... cells with stem-cell-like potential. Until now, there was no ...
Breaking Biology News(10 mins):Whitehead Institute researchers create 'naïve' pluripotent human embryonic stem cells 2Experiments prove 'stemness' of individual immune memory cells 2Experiments prove 'stemness' of individual immune memory cells 3
... 2013 The Damon Runyon Cancer Research Foundation ... its spring 2013 Clinical Investigator Award Committee review. ... are outstanding early career physician-scientists conducting patient-oriented cancer ... of the nation,s leading scientists and clinicians. ...
... other conditions caused by malfunctions in the body,s nerve ... treat. The problem is that one medicine may help ... which drugs will work with individual patients may be ... seemingly identical neurons can behave the same even though ...
... discovered a mechanism that triggers chronic inflammation in ... today in Nature Immunology , suggest a ... the way to a new class of therapies ... diseases without crippling the immune system. Alzheimer,s, atherosclerosis ...
Cached Biology News:Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 2Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 3Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 4Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 5Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 6Damon Runyon Cancer Research Foundation awards $3.6 M to 9 top young clinical investigators 7Nerve cells can work in different ways with same result 2Researchers discover new way to block inflammation 2
(Date:7/24/2014)... and synthetic processes prefer to settle into equilibriuma ... is within the realm of non-equilibrium conditions where ... in energy and phases, such as temperature fluctuations, ... humans to regulate their body temperature, airplanes to ... activity. , But even though these conditions ...
(Date:7/24/2014)... , July 24, 2014  CorMedix Inc. (NYSE ... and commercializing therapeutic products for the prevention and ... receipt of notice on July 18, 2014 from ... has accepted the CorMedix plan to regain compliance ... a result, the NYSE-MKT is continuing the Company,s ...
(Date:7/24/2014)... balances – 4 steps – One Recommendation: the web-based ... narrows down the mass of product choices to a ... , First, select the working environment, then enter the ... define the accuracy – it’s really that simple. Specifying ... to models that fit best with the customer’s industry ...
(Date:7/24/2014)... NJ (PRWEB) July 24, 2014 As ... manage numerous projects in the MENA region during 2014 ... have a preferred provider agreement with DZS to manage ... MENA region,” said Global VP of R&D and President ... has developed excellent operational practices with a focus on ...
Breaking Biology Technology:New approach to form non-equilibrium structures 2CorMedix, Inc. Granted Extension of NYSE-MKT Listing 2CorMedix, Inc. Granted Extension of NYSE-MKT Listing 3SBI Pharmaceuticals Co. Ltd. Enters into Preferred Provider Agreement with DZS Clinical Services 2
... 2011 Palatin Technologies, Inc. (NYSE Amex: PTN ) ... of shares of its common stock and warrants to purchase common ... market conditions and there can be no assurance as to whether ... actual size or terms of the offering. Roth Capital ...
... GREAT NECK, N.Y., Feb. 23, 2011 Scarguard Labs, ... of scars, psoriasis and other dermatological problems through the ... delivery system, announced today that they have received approval ... consumers throughout Canada. "We are very pleased ...
... Quanterix Corporation, a single molecule diagnostics company providing ... in vitro diagnostics markets, today announced the online publication ... Si ngle Mo lecule A rray (SiMoA™) ... straightforward measurement.  The article appears in the journal ...
Cached Biology Technology:Palatin Technologies, Inc. Announces Proposed Public Offering of Common Stock and Warrants 2Palatin Technologies, Inc. Announces Proposed Public Offering of Common Stock and Warrants 3Quanterix Announces Publication of Novel Method Expanding Single Molecule Detection Capability 2
... Intended Use: Vision Mount is formulated for covering tissue ... soluble OR alcohol insoluble end products. , ... ... chromogens such as Fast-Red or AEC that require an aqueous ...
... potent Rnase free Ribonuclease Inhibitor ... broad range of temperatures and ... not inhibit SP6, T7, or ... M-MLV reverse transcriptase or Taq ...
... kit, 110 to 220 V, enables ... doubles flow rates and valve capacity ... kit includes the Maximizer base unit, ... tubing kit, system cable 30, and ...
Mouse monoclonal antibody to QDPR - quinoid dihydropteridine reductase...
Biology Products: