David E. Fisher, MD, PhD, and his Dana-Farber colleagues describe their findings in Nature Biotechnology, which has published the paper as an advanced online publication on its Web site, www.nature.com/nbt/journal/vaop/ncurrent/index.html.
"This study presents the first global view of human nucleosome positioning," said Fisher. Although analyses of this type had been pioneered in simpler organisms such as yeast, those approaches were not feasible when applied to the massively larger and more complex human genome.
The new technique "provides major new clues to the locations of many hallmark features of the human genome, such as where transcription factors bind, where transcription begins and possibly ends, and where there are other biologically important structural features," said Fisher. Transcription factors are proteins that bind to particular DNA sites in "promoter" regions of genes and turn the genes on or off.
The novel method employed gene microarray studies followed by sophisticated computational data analysis to pin down the nucleosome locations. The paper describes how the scientists used the technique to locate nucleosomes in 3,692 promoters (regions of DNA that interact with regulatory factors to control gene activity) within seven human cell lines, including malignant melanoma.
Nucleosomes are spherical packing units for DNA. They consist of a length of
Source:Dana-Farber Cancer Institute