Navigation Links
Scientists identify specific enzymes that make meningitis hard to fight

Two enzymes in meningitis bacteria which prevent the body from successfully fighting off the disease, and make the infection extremely virulent, have been identified in new research published today.

The study found that the two enzymes - which have distinct functions - work together to hamper the body's efforts to fight off the disease. Together they repair damage done to the meningitis bacteria's DNA by the body's white blood cells, which are sent to fight the infection.

Understanding the part these enzymes play in the process that enables the bacteria to elude the body's natural defences could eventually help scientists develop novel new treatments for meningitis and the septicaemia it can cause. Both are extremely serious conditions with a high mortality rate, which take hold quickly and are difficult to treat - sometimes resulting in extreme measures such as limb amputation.

When the meningitis bacteria enter the bloodstream, the body's natural defences send white blood cells to fight the infection. They ingest the bacteria and subject them to oxidative stress. Oxidative stress damages the base chemical compounds of the bacteria's DNA. This should lead to cell death and the defeat of the bacteria. However, the virulent meningitis bacteria are able to repair this harmful damage and are therefore unaffected by the body's defences.

The research team found that the two enzymes essential to the bacteria's repair mechanism are AP endonuclease and 3'-phosphodiesterase. Contrary to the scientists' expectations, these two enzymes carry out separate functions in the DNA repair process.

Professor Paul Freemont from Imperial's Division of Molecular Biosciences explains: "Scientists have long understood that the secret of meningitis' virulence lies in its ability to rearrange its DNA and thus change its external appearance to the body's immune system. Significantly our research has now demonstrated that the ability of meningit is to repair its DNA is important for its ability to survive attack from the body's immune system. This work provides an insight into the precise roles of two enzymes in mending DNA and gives us a greater understanding than ever before into why these infections are so hard for the body to fight.

"We have also discovered that this novel combination of enzymes is present in a wide variety of bacteria and that it has a direct impact on the infectivity of human pathogens."

Dr Geoff Baldwin from Imperial's Division of Molecular Biosciences added: "The wide importance of DNA repair to the viability of all organisms is well established. However, for the first time this research shows an important role for DNA repair in meningitides fighting off the attack of the body's immune system.

"We have examined two enzymes from a family that are known to cut the DNA during its repair. To our surprise we discovered that in the meningitis bacteria, one of the enzymes does not cut the DNA. Instead, it removes damaged DNA ends that prevent the DNA from being remade during the repair process. This has an important impact for our understanding of the precise pathways of DNA repair and the significance of the two separate functionalities that act in different DNA repair pathways within the pathogen."

Professor Christoph Tang from Imperial's Division of Investigative Science added: "After realising that the two enzymes had distinct roles repairing different types of DNA damage, were able show the pathogen can 'fend off' different types of DNA damage while in the bloodstream. In theory, finding some way of disabling these enzymes would render the bacteria unable to repair itself, and therefore vulnerable to attack and defeat by the body's immune system."
'"/>

Source:Imperial College London


Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/23/2017)... robotic gym for the rehabilitation and functional motor sense evaluation of lower ... . The first 30 robots will be available from June in ... The technology was developed and patented at the IIT laboratories and has ... to a 10 million euro investment from entrepreneur Sergio Dompè. ... ...
(Date:4/19/2017)... York , April 19, 2017 ... as its vendor landscape is marked by the presence ... market is however held by five major players - ... Together these companies accounted for nearly 61% of the ... the leading companies in the global military biometrics market ...
(Date:4/11/2017)... PALM BEACH GARDENS, Fla. , April 11, ... biometric identity management and secure authentication solutions, today ... million contract by Intelligence Advanced Research Projects Activity ... technologies for IARPA,s Thor program. "Innovation ... the onset and IARPA,s Thor program will allow ...
Breaking Biology News(10 mins):
(Date:9/21/2017)... (PRWEB) , ... September 21, 2017 , ... ... HQ office, which includes executive, engineering and manufacturing functions to The LaunchPort™ Accelerator ... The Factory™, provide a full range of manufacturing and business services to its ...
(Date:9/21/2017)... Jersey (PRWEB) , ... September 21, 2017 , ... ... pharmaceutical and clinical research sector professionals, has announced the addition of 5 new ... 11 training - Compliance with Regulation 21 CFR Part 11 on Electronic ...
(Date:9/20/2017)... New Haven, CT (PRWEB) , ... September 20, ... ... therapeutics company, announced today that it has appointed Vishwas Paralkar to the role ... of Cybrexa’s tumor targeting technology. He will report to Cybrexa’s president and CEO, ...
(Date:9/20/2017)... ... September 20, 2017 , ... From industry leaders, sports stars, and Hollywood icons ... Many of these people had lost all hope. Their stories are told ... (pdf) , “Neil takes readers on a riveting journey through the past, ...
Breaking Biology Technology: