Navigation Links
Scientists identify genetic pathways essential to RNA interference

A research team based at Massachusetts General Hospital (MGH) has identified 80 new genes essential to the process of RNA interference (RNAi), a powerful new research tool for inactivating genes in plants or animals. They used the RNAi process itself to find new genes that participate in the gene-silencing mechanism, which someday may help to fight human disease. The report will appear in the journal Science and is receiving early online release on the Science Express website at http://www.sciencexpress.org.

"The gene activation produced by RNAi is exquisitely specific, which gives it enormous potential for therapeutic application," says Gary Ruvkun, PhD, of the MGH Department of Molecular Biology, the study's senior author. "Imagine short, double-stranded RNA molecules that could be synthesized quickly and inexpensively to silence a single gene. Promising targets could include viruses like HIV and hepatitis C or cancer-causing oncogenes. An RNAi-based treatment for age-related macular degeneration is already in clinical trials." Ruvkun is a professor of Genetics at Harvard Medical School.

RNAi was originally identified in the C. elegans roundworm and the flowering plant Arabidopsis thaliana, both of which are common model organisms for biological research. The process interrupts the usual transfer of instructions from double-stranded DNA, through single-stranded messenger RNA and finally into proteins. Short, double-stranded pieces of RNA bind to the complementary messenger RNA segments, shutting down gene expression. RNAi occurs naturally in plants and animals and may help control resistance to viral infection, among other functions.

For the current study, lead author John Kim, PhD, and his colleagues developed a strain of C. elegans into which they added a gene that caused the worms to glow under ultraviolet light but also turned that gene off using RNAi. They then used RNAi to inactivate every one of the worms' 19,000 genes by feeding the worms bacteria that produce double-stranded RNA for each gene. Inactivation of about 90 genes caused the worms to glow, indicating that those genes were essential to the RNAi process that had been suppressing expression of the fluorescence gene.

Some of the identified genes - many of which have human counterparts - code for proteins involved with the packaging and processing of RNA, but others may be involved with the regulation of DNA itself, including the repair of DNA damage. "These new steps indicate there is more to RNAi than RNA destruction," says Kim. "And the connection to DNA damage pathways, which was totally unexpected, suggests a potential connection between RNAi and the control of cell division in cancer."

The researchers note that better understanding the mechanisms underlying RNAi could help transform what has been a research tool into a powerful therapeutic tool. Although the process has worked well in studies of cultured human cells, it has not yet been effective for experimentally suppressing gene expression in living mammals. Identifying each step in the RNAi process could lead to more successful inactivation of disease-related genes. And in addition to the technique's potential for gene silencing, controlling levels of RNAi that may underlie some cancers or be used in viral replication may offer further clinical potential.

Along with Kim, the study's co-first authors are Harrison Gabel and Ravi Kamath, MD, PhD, of the MGH Department of Molecular Biology. Additional authors are Muneesh Tewari, MD, PhD, Jean-Francois Rual, Nicolas Bertin, and Marc Vidal, PhD, of Dana-Farber Cancer Institute; Amy Pasquinelli, PhD, of the University of California at San Diego; Scott Kennedy, PhD, of the University of Wisconsin; and Michael Dybbs and Joshua Kaplan, PhD, of MGH. The research was supported by grants from the National Institutes of Health.

Massachusetts General Hospital, established in 1811, is the original and largest tea ching hospital of Harvard Medical School. The MGH conducts the largest hospital-based research program in the United States, with an annual research budget of more than $450 million and major research centers in AIDS, cardiovascular research, cancer, cutaneous biology, medical imaging, neurodegenerative disorders, transplantation biology and photomedicine. In 1994, MGH and Brigham and Women's Hospital joined to form Partners HealthCare System, an integrated health care delivery system comprising the two academic medical centers, specialty and community hospitals, a network of physician groups, and nonacute and home health services.


'"/>

Source:Massachusetts General Hospital


Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/3/2016)... 2016 Das DOTM ... Nepal hat ein 44 Millionen ... Kennzeichen, einschließlich Personalisierung, Registrierung und IT-Infrastruktur, an ... und Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte internationale ... teilgenommen, aber Decatur wurde als konformste und ...
(Date:5/24/2016)... Calif. , May 24, 2016 Ampronix facilitates superior patient care by ... LMD3251MT  3D medical LCD display is the latest premium product recently added to the ... ... ... Sony 3d Imaging- LCD Medical Display- Ampronix News ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... , June 23, 2016   Boston ... of novel compounds designed to target cancer stemness ... has been granted Orphan Drug Designation from the ... treatment of gastric cancer, including gastroesophageal junction (GEJ) ... inhibitor designed to inhibit cancer stemness pathways by ...
(Date:6/23/2016)... 23, 2016 Houston Methodist Willowbrook Hospital ... Sports Association to serve as their official health ... Methodist Willowbrook will provide sponsorship support, athletic training ... association coaches, volunteers, athletes and families. ... Sports Association and to bring Houston Methodist quality ...
(Date:6/23/2016)... -- The Biodesign Challenge (BDC), a university competition that asks ... systems and biotechnology, announced its winning teams at the ... York City . The teams, chosen ... MoMA,s Celeste Bartos Theater during the daylong summit. Keynote ... of architecture and design, and Suzanne Lee , ...
(Date:6/23/2016)... OTTAWA, ON (PRWEB) , ... June 23, 2016 , ... ... former DNA Technical Leader at the Arkansas State Crime Laboratory, has joined STACS DNA ... joining the STACS DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. ...
Breaking Biology Technology: