Navigation Links
Scientists identify genes responsible for 'black rot' disease in vegetables

Scientists at four major genomics and plant pathology laboratories in China have collaborated on a project to characterize the causative agent of "black rot" disease, which is the most serious disease of vegetable crops worldwide. Their study, which represents the largest comparative and functional genomics screen for a plant or animal bacterial pathogen to date, is published online today in the journal Genome Research.

"Black rot" is caused by the pathogenic bacterium Xanthomonas campestris pathovar campestris (or Xcc). Under favorable conditions (high humidity and temperature), Xcc infects vegetable crops by spreading through the plants' vascular tissues, turning the veins in their leaves yellow and black, and causing V-shaped lesions along the margins of the leaves. All vegetables in the crucifer family, including broccoli, Brussels sprouts, cabbage, cauliflower, kale, mustard, radish, rutabaga, and turnip, are potential hosts for Xcc. The model plant Arabidopsis thaliana is also susceptible to Xcc infection. Surprisingly, however, some wild cruciferous weed species do not manifest the characteristic symptoms of "black rot" disease when infected.

To date, there is no effective treatment for Xcc infection, so in hopes of developing a treatment, scientists at four Chinese institutions (the Institute of Microbiology at the Chinese Academy of Sciences, the Chinese National Human Genome Center at Shanghai, Guangxi University, and the Chinese National Human Genome Center at Beijing) have focused their efforts on characterizing the genes responsible for Xcc pathogenicity. In their study published today, the investigators describe the identification of 75 different genes responsible for Xcc virulence. These genes appear to belong to 13 different functional categories or related metabolic pathways. The researchers hope that the molecular characterization of these pathogenicity-related genes will lead to the development of a treatment for "black rot" disease .

Employing whole-genome comparative genomic approaches, the authors sequenced the complete genome of an Xcc strain that was isolated from an infected cauliflower plant in England during the 1950's. They then compared this sequence to a previously published sequence from a cabbage-derived Xcc strain. Although the gene content of the two strains was very similar, the authors identified several genes located on strain-specific chromosomal elements that were unique to each strain. In addition, there were dramatic differences in the genomic arrangement of the two strains; the scientists identified significant rearrangements between the genomes, including major translocations, inversions, insertions, and deletions.

In order to functionally characterize Xcc and identify genes implicated in its pathogenicity, the researchers then screened an Xcc transposon insertional mutant library in its host plant (cabbage). They screened a total of 16,512 Xcc mutants on individual cabbage plants and, of these, 172 proved to be non-pathogenic. Upon further characterization of the 172 non-pathogenic mutants, the researchers came up with a non-redundant list of 75 genes or non-coding regions that are involved in Xcc pathogenicity.

Interestingly, the researchers identified three genes that were implicated in pathogenicity but that were not present in the previously described Xcc genomic sequence. To test the biological implications of this observation, they inoculated five different vegetable species with the three mutants corresponding to these strain-specific genes, and they observed significant differences in the response of each host species to infection. The authors point out that these findings highlight the role of genome dynamics in the evolution of pathogenicity in Xcc in response to different host species.


'"/>

Source:Cold Spring Harbor Laboratory


Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/31/2016)... , March 31, 2016 ... ) ("LegacyXChange" or the "Company") LegacyXChange is ... users of its soon to be launched online site ... https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide potential shareholders ... of DNA technology to an industry that is notorious ...
(Date:3/23/2016)... , March 23, 2016 ... erhöhter Sicherheit Gesichts- und Stimmerkennung mit Passwörtern ... (NASDAQ: MESG ), ein führender ... das Unternehmen mit SpeechPro zusammenarbeitet, um erstmals ... Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, im Rahmen ...
(Date:3/21/2016)... 2016 Unique technology combines ... superior security   Xura, Inc. ... secure digital communications services, today announced it is working ... enterprise customers, particularly those in the Financial Services Sector, ... authentication within a mobile app, alongside, and in combination ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... Newly ... technologies, services and solutions to the healthcare market. The company's primary focus is ... manufacturing, sales and marketing strategies that are necessary to help companies efficiently bring ...
(Date:6/27/2016)...   Ginkgo Bioworks , a leading organism ... today awarded as one of the World Economic ... most innovative companies. Ginkgo Bioworks is engineering biology ... world in the nutrition, health and consumer goods ... customers including Fortune 500 companies to design microbes ...
(Date:6/24/2016)... ... ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension of 8.5 ... end machines that use the more unconventional z-dimension of 20mm. Z-dimension or ... the cuvette holder. , FireflySci has developed several Agilent flow cell product lines ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... is pleased to announce the launch of their brand, UP4™ Probiotics, into Target ... over 35 years, is proud to add Target to its list of well-respected ...
Breaking Biology Technology: