Navigation Links
Scientists force viruses to evolve as better delivery vehicles for gene therapy

e could address as well, such as targeting the virus to cells it is ordinarily not good at getting into, or speeding its transport through the body," he said.

Though Schaffer acknowledges that the technique could be used to help pathogenic viruses evade the human immune system, potentially making them more virulent, he said that other and easier techniques already allow this frightening possibility.

AAV consists of two genes enclosed within a ball, or capsid, of proteins. The capsid proteins are what antibodies recognize, and as a result were the target of directed evolution. To provide the raw material for evolution - the genetic variation from which nature selects the best-adapted organism - the researchers created mutant viruses by introducing small variations in the genes through an error-prone polymerase chain reaction (PCR) coupled with a test tube recombination technique. After reassembling the mutant viruses inside their capsids, they introduced them to blood serum pooled from rabbits immunized against AAV, and thus containing many types of antibodies to AAV. Only the mutant viruses good at evading antibodies to AAV survived the serum.

After passing the viruses three times through increasingly more potent serum, they isolated the survivors and subjected them to another round of PCR that introduced more mutations. After passing this second generation through serum three times, they isolated viruses that could survive AAV antibodies much better than the original strain of AAV. One strain of virus was 96 times more effective than the wild AAV, and two evolved strains survived injection into mice with nearly 1,000 times the level of antibodies required to neutralize the wild virus.

By sequencing the survivor strains, the researchers discovered that the capsid proteins of the survivors differed from those of the original strain by only seven amino acid building blocks, two of which were responsible for most of the altered interacti
'"/>

Source:University of California - Berkeley


Page: 1 2 3

Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
(Date:8/21/2014)... fungus linked to dandruff, eczema and other itchy, flaky ... further global reachesincluding Hawaiian coral reefs and the extreme ... A review in the scientific journal PLOS Pathogens ... fungi of the genus Malassezia in light ... from around the world. , University of Hawai,i at ...
(Date:8/21/2014)... is a key region in the global oceanic circulation. "On ... warm Atlantic water flows to the north into the Arctic ... and sea ice push their way out of the Arctic ... water cools here on its way to the north and ... in this manner drives the global band of oceanic currents ...
(Date:8/21/2014)... of researchers at Louisiana Tech University has ... consumer-grade 3D printers and materials to fabricate ... and chemotherapeutic compounds for targeted drug delivery. ... and research faculty from Louisiana Tech,s biomedical ... create filament extruders that can make medical-quality ...
Breaking Biology News(10 mins):From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2Arctic sea ice influenced force of the Gulf Stream 2Arctic sea ice influenced force of the Gulf Stream 3Louisiana Tech University researchers use 3D printers to create custom medical implants 2Louisiana Tech University researchers use 3D printers to create custom medical implants 3
... CA August 27, 2013 A recently reported ... injury could enable more timely therapeutic intervention in transplant ... As described by scientists at the University Medical Center ... new method uses Bio-Rad Laboratories, Droplet Digital PCR ...
... A team of scientists from the UCLA Departments of ... a three-year, $2 million grant from the W.M. Keck ... of donor livers. Dr. Jerzy W. Kupiec-Weglinski, principal ... Transplantation Research Center, said he hopes his research will ...
... member of a National Research Council (of the National Academies) ... is through Nov. 14, 2013. The task includes providing ... ocean surface following spills. Such guidelines are important for ... a notable voice in the investigation of the Exxon Valdez ...
Cached Biology News:Researchers develop rapid, cost-effective early detection method for organ transplant injury 2Researchers develop rapid, cost-effective early detection method for organ transplant injury 3UCLA scientists receive $2 million grant to improve quality of donor livers for transplant 2NJIT professor appointed to National Research Council 2
(Date:8/22/2014)... 2014 Today the Academy of Model Aeronautics ... Court of Appeals for the D.C. Circuit challenging the FAA’s ... the Federal Register on June 25, 2014. , The ... the text and intent of the FAA Modernization and Reform ... prohibition on any new rules or regulations regarding model aircraft ...
(Date:8/22/2014)... for application as catalysts, sensors, drug delivery ... photonics and molecular electronics. Gaining knowledge of ... and chemical properties, has been challenging. Now, ... that high-resolution electron microscopy can be used ... all gold atoms are observed. The results ...
(Date:8/22/2014)... IN (PRWEB) August 22, 2014 The ... community and its members in Alaska that Alaska Governor ... Concurrent Resolution 15, further defining public use of Unmanned ... Systems Task Force. House Bill 255states that it is, ... to images captured by an unmanned aircraft system.” It ...
(Date:8/21/2014)... MD (PRWEB) August 21, 2014 DNA ... making progress in its development of JRC-LAMP-vax, an innovative ... cedar pollen. Phase IC studies began this month in ... to hay fever. , The Japanese red cedar releases ... people in Japan. Mountain Cedar pollen wreaks similar havoc ...
Breaking Biology Technology:AMA Files Petition for Review of FAA’s Interpretive Rule 2A breakthrough in imaging gold nanoparticles to atomic resolution by electron microscopy 2State of Alaska Signs Unmanned Aircraft Systems Bill 2Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 2Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 3Relief for Allergy Sufferers Finally in Sight: Immunomic Therapeutics Closes in on Treatment for Hay Fever in Japan, U.S. 4
... Shire plc (LSE: SHP, NASDAQ: SHPGY ), the global ... April 26, 2012. Results press release will be ... 14:00 BST / 09:00 EDT, , Live ... and Graham Hetherington, Chief Financial Officer will host the investor and ...
... 12, 2012  The Orthopedic Design & Technology Forum will ... of Technology in Memphis, Tenn. Among the critical topics ... will address future trends in biologics—design implications, commercialism and ... the field of biologics: inductive proteins, conductive substrates, and ...
... Kan., April 11, 2012 Riordan Clinic ... Ronald Hunninghake have patented a new technology that may ... patients.  The research, recently published in the January/February issue ... "The Assessment of the Energy Metabolism in Patients with ...
Cached Biology Technology:Shire plc: First Quarter 2012 Results Date Notification - April 26, 2012 2ODT Forum Presents Regenerative Technologies 2Riordan Clinic Research Institute Has Developed and Patented a Process for the Assessment of the Energy Metabolism in Patients with Chronic Fatigue Syndrome 2Riordan Clinic Research Institute Has Developed and Patented a Process for the Assessment of the Energy Metabolism in Patients with Chronic Fatigue Syndrome 3
... The CellSensor CRE-bla HEK 293T cell line ... of the cAMP response element (CRE). To ... was transduced into HEK 293T cells by ... isolate clones responsive to forskolin stimulation (Figure ...
...
2ndGen Predilute antibody for staining tissue sections. Optimized for use with Zymeds Histostain-PLUS, NBA, HistoST5050, and Cap-Plus IHC Kits.Labeling: ASR...
... Bean Nuclease catalyzes the degradation of single-stranded ... terminated products. While the nuclease prefers ssDNA ... concentrations the enzyme degrades double-stranded DNA from ... been used for transcript mapping studies (3,4), ...
Biology Products: