Navigation Links
Scientists Replicate Hepatitis C Virus in Laboratory

For the first time, scientists have replicated hepatitis C virus (HCV) in the laboratory. The ability to replicate HCV in cell culture will allow researchers to better study the life cycle and biology of this virus and to test potential antiviral compounds, which may lead to new therapies for the liver disease that results from infection with HCV. Scientists at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), one of the National Institutes of Health (NIH), conducted the study, which appears in the Feb. 15, 2005 issue of Proceedings of the National Academy of Sciences (PNAS).

“Until recently, research on this infectious disease has suffered from the lack of a robust in vitro model system,?says T. Jake Liang, M.D., Chief of the Liver Diseases Branch of the NIDDK and co-author of the study. “Our model system produced viral particles that have all the properties of the whole virus. This evidence together with an analysis of the replicated viral RNA supports a conclusion of viral replication and production.?/p>

The NIDDK group used a strain of HCV that would have applications to the greatest number of people ?genotype 1, the major type of HCV of human infections worldwide and the type most resistant to current therapies. They constructed an HCV replica using a DNA copy of the original HCV single-strand RNA genome. They placed the DNA copy between two ribozymes, RNA molecules that have enzymatic function and can cleave RNA sequence at specific locations. These two ribozymes were designed to generate the correct ends of the HCV genome and to act as start and stop buttons to gene activity. The construct was “naked,?meaning that it contained only nucleic acids, the genetic material of the virus, and did not have the HCV viral envelope, a protective shell of lipids and proteins that surrounds the viral RNA in fully-formed HCV. The naked HCV construct was then placed into human liver cells in a cell culture medium.

The NIDDK scie
'"/>

Source:NIH News


Page: 1 2

Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists detect probable genetic cause of some Parkinsons disease cases
3. Scientists find missing enzyme for tuberculosis iron scavenging pathway
4. Scientists seek answers on what activates deadly anthrax spores
5. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
6. Scientists collaborate to assess health of global environment
7. Scientists decipher genome of fungus that can cause life-threatening infections
8. Scientists discover the cellular roots of graying hair
9. Scientists rid stem cell culture of key animal cells
10. Scientists develop new color-coded test for protein folding
11. Scientists identify genetic pathways essential to RNA interference
Post Your Comments:
(Date:5/15/2008)...15, 2008 (BRONX, NY) Contrary to long-held assum...of death, according to investigators from the Albe...ity. They reached their conclusion after examining...sample of adults in the U.S. The Einstein research...sk of death from cardiovascular disease (CVD) asso...
(Date:5/15/2008)...nne, Ill. (May 15, 2008) Argonne National Laborat... with Savannah River National Laboratory (SRNL) to...anagement research projects in support of critical...rtant national priorities. , "The MOU allows Arg...," Argonne director Robert Rosner said. "Argonne h...
(Date:5/15/2008)...ermen hold empirical knowledge that tuna aggregate...pe, pieces of wood, or even large marine mammals. ...ation behaviour, but the past 20 years have seen p... associated concentrations of fish. Fishermen cast...as FADs. An enormous purse-seine net, deployed in ...
(Date:5/14/2008)...telligent Wireless Module Combines Linux, Security...mption, TUSTIN, Calif., May 14 /PRNewswire/ -- Si...lligent high-performance module designed for conne...o 802.11a/b/g wireless local,area networks (WLANs)...d,WLAN connectivity with minimal power usage, such...
Breaking Biology News(10 mins):New study casts further doubt on risk of death from higher salt intake 2Argonne-SRNL agreement supports critical DOE, national priorities 2Does fishing on drifting fish aggregation devices endanger the survival of tropical tuna? 2Does fishing on drifting fish aggregation devices endanger the survival of tropical tuna? 3New Silex SX-560 Enables Enterprise 802.11 Wireless Connectivity for Battery Operated Devices 2Joint NJ NY Rally for Addiction Recovery to Be Hub of Events Nationwide 1072 1Amicus Therapeutics to Present at the Merrill Lynch Global Pharmaceutical Biotech 26 Medtech 2007 Conference 521 1Amicus Therapeutics to Present at the Merrill Lynch Global Pharmaceutical Biotech 26 Medtech 2007 Conference 521 2Amicus Therapeutics to Present at the Merrill Lynch Global Pharmaceutical Biotech 26 Medtech 2007 Conference 521 3Senior Research Analyst Richard Evans Joins AVOS Life Sciences 518 1Senior Research Analyst Richard Evans Joins AVOS Life Sciences 518 2Phosphagenics to Commence Phase 2 TPM Insulin Clinical Trial 253 1Phosphagenics to Commence Phase 2 TPM Insulin Clinical Trial 253 2Phosphagenics to Commence Phase 2 TPM Insulin Clinical Trial 253 3
...ormation involves the carefully timed production o...lls: neurons are produced first, then astrocytes. ... of another at a given time could lead to brain ma...rchers in the Neurobiology Program at Children,s H...pathway that influences the timing of nerve-cell p...
...etic basis for a rare inherited disorder that caus...npointed by researchers. They found that paroxysma...ic mutations in porelike sodium channels in periph...izes the role of such channel disorders in inflamm...disease also provide insights into the normal func...
...hers at the University of Illinois at Chicago Coll...es determine how well an antipsychotic medication ...it will cause. , Risperidone, a popular "atypical"... illnesses such as schizophrenia and bipolar disor...armacy practice, is examining the effects of one g...
...eat stem sawfly natural enemies in demand, Montana...ways of increasing their availability. , This fa...udy that involved mass-rearing parasitic wasps tha...interior of developing wheat plants. The team incl... Godshen Pallipparambil-Robert and undergraduate M...
Other Biology News:Novel pathway regulates timing of brain cell development 2Origin of inherited pain disorder pinpointed 2Studies look at how genes affect antipsychotic drug response 2Rearing an army to save wheat 2Rearing an army to save wheat 3
(Date:5/15/2008)...roduct Sales Increased 149% to $3.5 Million - ... TUSTIN, Calif., May 15 /- ...d specialty pharmaceutical company headquartered,i... Jiangxi and Jilin,China. Through its wholly owned...mpany engages in the research, development, manufa...
(Date:5/15/2008)...Reached to Terminate Marketing Agreement with Tria... Favorable Terms, NAPA, Calif., May 15 /PRNewswir...NKTY), a Life Sciences company engaged in the deve... healthy aging, today announced,financial results ...ent in,principle to terminate its marketing agreem...
(Date:5/15/2008)...us condition of esophagus eliminated in 90 percent...ALE, Calif., May 15 /- BARRX Medical,...recancerous conditions of the,digestive tract, tod...a,multi-center U.S. study were free of the highest...rgical, endoscopic treatment using the,HALO360 abl...
(Date:5/15/2008)...d 19.4% year-over-year to $2.3 million or 11 cents...AN, Shandong, China, May 15 /Xinhua-PRNewswire-Fir...etin Board: CBPO) ("CBP" or the,"Company"), one of... in the,People,s Republic of China ("PRC"), today ...nded on March 31, 2008., First Quarter 2008 Hig...
Breaking Biology Technology:AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 2AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 3Senetek PLC Reports First Quarter 2008 Financial Results 2Senetek PLC Reports First Quarter 2008 Financial Results 3New Study Reports That Ablative Therapy With HALO360 Device Effectively Treats Highest-Risk Category of Barrett's Esophagus 2New Study Reports That Ablative Therapy With HALO360 Device Effectively Treats Highest-Risk Category of Barrett's Esophagus 3China Biologic Products Reports First Quarter 2008 Results 2China Biologic Products Reports First Quarter 2008 Results 3China Biologic Products Reports First Quarter 2008 Results 4China Biologic Products Reports First Quarter 2008 Results 5China Biologic Products Reports First Quarter 2008 Results 6China Biologic Products Reports First Quarter 2008 Results 7China Biologic Products Reports First Quarter 2008 Results 8China Biologic Products Reports First Quarter 2008 Results 9China Biologic Products Reports First Quarter 2008 Results 10
... A "sister park" agreement has been announced bet... the Frankfurt Biotechnology Innovation Center in ..., ,A memorandum of understanding between the two r...n Chancellor John Wiley, University Research Park ... director of the Frankfurt Biotechnology Innovatio...
... Careers in manufacturing are high-wage and high-t...e horizon. It appears a negative public perception...e work and lay-offs - is thwarting young adults fr...ry. This despite a projected need for 10 million n...nal Association of Manufacturers (NAM). , ,In Wisc...
...w prices. When it comes to consumer goods, America...roduct selection and fanatical commitment to low p...ing. We like our stuff cheap, and Wal-Mart is the ...s hate to love is getting loads of unfavorable att...essfully derailed its growth plans in certain part...
... Stunden, chief information officer and director o... the University of Wisconsin-Madison, has announce...t the helm of campus technology. , ,"We have made ... Stunden said. "Above all, I think we have strengt... and built a more collaborative model." , , Stun...
Other Biology Technology:State campaign seeks to reverse negative perception of manufacturing work 2State campaign seeks to reverse negative perception of manufacturing work 3State campaign seeks to reverse negative perception of manufacturing work 4State campaign seeks to reverse negative perception of manufacturing work 5Wal-Mart: How low can you go? 2Wal-Mart: How low can you go? 3Wal-Mart: How low can you go? 4Stunden to retire as UW-Madison CIO 2
Normal Human Serum from CHEMICON
X-Gal from Research Organics, Inc.
GIBCO Advanced D-MEM (Dulbecco's Modified Eagle Medium) (1X) liquid from Invitrogen
Agar from Calbiochem
Biology Products: