Navigation Links
Scientist uses form to explain function of key building blocks of life

University of Wisconsin-Madison biochemists have developed an approach that allows them to measure with unprecedented accuracy the strengths of hydrogen bonds in a protein. The scientists were then able to predict the function of different versions of the protein based on structural information, a novel outcome that was published recently in the Proceedings of the National Academy of Sciences.

Professor of biochemistry John Markley, along with a team that included graduate student I-Jin Lin, studied iron-sulfur proteins called rubredoxins that transfer energy in the form of electrons throughout living systems.

Rubredoxin is a key part of processes like photosynthesis and respiration, where energy is converted from one form to another.

"Variants of rubredoxin have evolved different sequences to transport electrons in the most efficient manner possible," Markley explains. "Different mechanisms have been put forward to explain this, and we wanted to understand how the proteins evolved to have different electron affinities."

Markley and his team used nuclear magnetic resonance spectroscopy, a technique that allowed them to observe signals from atoms in the proteins, to determine the strength of hydrogen bonds in ten different variants of the protein. From that data, the team was able to explain changes in protein function.

"In science, you try to build theories that will explain the properties of the systems you are looking at," explains Markley. "Proteins are the basic building blocks of life, and are coded for by the genes in DNA. We'd like to be able to start with a gene sequence and predict the structure of a protein and its function. In this case, given an NMR pattern, we can tell you how the protein will act. In general, this method may provide information about even more complex biological systems. This is an approach that will be important for larger proteins."

Markley notes that an undergraduate and graduat e student played key roles in the study. Lin, who plans to complete her Ph.D. this spring, spent years tackling what Markley described as a "complex and difficult project."

Erika Gebel, the undergraduate on the study, is now pursuing a graduate degree of her own, a pursuit that was enhanced by this project, says Markley.

"(Undergraduate research) enables them to understand what research is and what's involved in exploring something that hasn't been observed before," he says.


'"/>

Source:University of Wisconsin-Madison


Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... Apr. 11, 2017 Research and Markets has ... report to their offering. ... The global eye tracking market to grow at a CAGR of ... Eye Tracking Market 2017-2021, has been prepared based on an in-depth ... market landscape and its growth prospects over the coming years. The ...
(Date:4/11/2017)... April 11, 2017 NXT-ID, Inc. (NASDAQ: ... company, announces the appointment of independent Directors Mr. Robin ... its Board of Directors, furthering the company,s corporate governance and ... Gino Pereira ... look forward to their guidance and benefiting from their considerable ...
(Date:4/5/2017)...  The Allen Institute for Cell Science today announces ... portal and dynamic digital window into the human cell. ... application of deep learning to create predictive models of ... a growing suite of powerful tools. The Allen Cell ... publicly available resources created and shared by the Allen ...
Breaking Biology News(10 mins):
(Date:5/22/2017)... , ... May 22, 2017 , ... ... that it is exhibiting in booth B2 at the Association for Pathology ... 22-25. , In addition to demonstrating its Cancer Diagnostic Cockpit and Consultation ...
(Date:5/19/2017)... ... May 19, 2017 , ... The University ... researchers with technologies ripe for commercialization, and who are affiliated with the 21 ... to submit proposals. QED, now in its tenth round, is the first multi-institutional ...
(Date:5/18/2017)... (PRWEB) , ... May 17, 2017 , ... NDA Partners ... executive and former CEO of Eurofins Advantar Laboratories and President of Pharmaceutical Development Business ... to his position at Eurofins and Cardinal Health, he was former Chief Operating Officer ...
(Date:5/18/2017)... ... May 17, 2017 , ... HOLLOWAY AMERICA, a leading ... and dairy, munitions, and pharmaceutical/biotech, recently introduced The Revolution Lift™, a new precision-controlled ... improvement in technology comes on the heels of HOLLOWAY’s release of the intelliVessel™, ...
Breaking Biology Technology: