Navigation Links
Scientist uses form to explain function of key building blocks of life

University of Wisconsin-Madison biochemists have developed an approach that allows them to measure with unprecedented accuracy the strengths of hydrogen bonds in a protein. The scientists were then able to predict the function of different versions of the protein based on structural information, a novel outcome that was published recently in the Proceedings of the National Academy of Sciences.

Professor of biochemistry John Markley, along with a team that included graduate student I-Jin Lin, studied iron-sulfur proteins called rubredoxins that transfer energy in the form of electrons throughout living systems.

Rubredoxin is a key part of processes like photosynthesis and respiration, where energy is converted from one form to another.

"Variants of rubredoxin have evolved different sequences to transport electrons in the most efficient manner possible," Markley explains. "Different mechanisms have been put forward to explain this, and we wanted to understand how the proteins evolved to have different electron affinities."

Markley and his team used nuclear magnetic resonance spectroscopy, a technique that allowed them to observe signals from atoms in the proteins, to determine the strength of hydrogen bonds in ten different variants of the protein. From that data, the team was able to explain changes in protein function.

"In science, you try to build theories that will explain the properties of the systems you are looking at," explains Markley. "Proteins are the basic building blocks of life, and are coded for by the genes in DNA. We'd like to be able to start with a gene sequence and predict the structure of a protein and its function. In this case, given an NMR pattern, we can tell you how the protein will act. In general, this method may provide information about even more complex biological systems. This is an approach that will be important for larger proteins."

Markley notes that an undergraduate and graduat e student played key roles in the study. Lin, who plans to complete her Ph.D. this spring, spent years tackling what Markley described as a "complex and difficult project."

Erika Gebel, the undergraduate on the study, is now pursuing a graduate degree of her own, a pursuit that was enhanced by this project, says Markley.

"(Undergraduate research) enables them to understand what research is and what's involved in exploring something that hasn't been observed before," he says.


'"/>

Source:University of Wisconsin-Madison


Related biology news :

1. Scientists ID molecular switch in liver that triggers harmful effects of saturated and trans fats
2. Scientists Replicate Hepatitis C Virus in Laboratory
3. Scientists detect probable genetic cause of some Parkinsons disease cases
4. Scientists find missing enzyme for tuberculosis iron scavenging pathway
5. Scientists seek answers on what activates deadly anthrax spores
6. Yale Scientists Find MicroRNA Regulates Ras Cancer Gene
7. Scientists collaborate to assess health of global environment
8. Scientists decipher genome of fungus that can cause life-threatening infections
9. Scientists discover the cellular roots of graying hair
10. Scientists rid stem cell culture of key animal cells
11. Scientists develop new color-coded test for protein folding
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/2/2016)... , March 2, 2016 ... announced the addition of the  "Global Biometrics ... their offering.  ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , , ... to grow at a CAGR of around ... ) has announced the addition of ...
(Date:3/1/2016)... 1, 2016 ... of the  "Global Biometric Access Control ... their offering. --> http://www.researchandmarkets.com/research/ghsgdt/global_biometric ... the  "Global Biometric Access Control Systems ... offering. --> Research and ...
(Date:3/1/2016)... and SAN FRANCISCO , March 1, ... Corp. and BitGo, Inc. extends biometric authentication to the ... private keys. Bitcoin transactions that ... per month in digital assets with over 10,000 transactions ... any startup. HYPR enables enterprises to keep encrypted biometric ...
Breaking Biology News(10 mins):
(Date:4/29/2016)... TURIN, Italy , April 29, 2016 ... version 5.11, the latest update to its industry-leading treatment ... has shown that Monaco version ... Users can now attain calculation speeds up to four ... Monaco . With the industry,s gold standard ...
(Date:4/28/2016)... Q BioMed Inc. (OTCQB:QBIO), ...  was featured in an article he wrote on ... To Tread: http://www.lifescienceleader.com/doc/accelerators-enter-when-vcs-fear-to-tread-0001 ... an essential business journal for life science executives ... Big Pharmas. Their content is designed to inform ...
(Date:4/28/2016)... ... April 28, 2016 , ... Next week on May 5 ... first-in-class technologies for tissue stem cell counting and expansion to gene-editing scientists and ... & CRISPR-based Genome Engineering in Burlington, Massachusetts. , The attention of most gene-editing ...
(Date:4/28/2016)... (PRWEB) , ... April 28, 2016 , ... ... Inc., will hold an open house for regional manufacturers at its Maple Grove, ... from Tsugami, Okuma, Hardinge Group, Chiron and Trumpf. Almost 20 leading suppliers ...
Breaking Biology Technology: