Navigation Links
Ring-like formations in drying DNA drops could affect hybridization studies

Coffee drinkers are familiar with the ring-shaped stains that result from spilled drops that have dried, in which the brown stain is not evenly distributed, but instead concentrated at the edge. Now, a team led by Gerard Wong, a professor of materials science and engineering, physics, and bioengineering at the University of Illinois at Urbana-Champaign has found the same "coffee-ring" formation in drying drops of DNA.

To gain insights into the physics behind the ring phenomenon, Wong's team experimentally studied the dynamics of drying DNA droplets on glass surfaces. They report their findings in a paper accepted for publication in the journal Physical Review Letters, and posted on its Web site.

"As the droplet evaporated, DNA chains were transported outward by water flow to the drop's perimeter," Wong said. "At the droplet edge, the DNA became increasingly concentrated and formed a liquid crystal with concentric chain orientations. (Liquid crystals are materials that flow like a liquid, but can align in a preferred direction like a crystalline solid.) During the final stages of drying, stresses propagated from the rim inward through the liquid crystal, creating cracks that formed a periodic zigzag pattern."

To examine the structure and behavior of the DNA liquid crystal, the researchers used a relatively new imaging technique developed at Kent State University. Called fluorescence confocal polarizing microscopy, the technique imaged the DNA in the drying droplet in three dimensions.

"The DNA alignment parallel to the droplet's edge was counterintuitive," Wong said. "We had expected the DNA to extend along the direction of flow, which was mainly in the radial direction. But, instead of resembling the spokes of a bicycle wheel, the transported DNA resembled the rim of a bicycle wheel."

Since nearly all the DNA is concentrated in a narrow ring with almost no DNA in the rest of the stain, these effects should be accounted for in t he design of arrays in which DNA droplets are sequentially deposited onto a glass surface for hybridization studies, the researchers report.

"Without optimization of the wetting conditions, it is possible to miss all the DNA in the ring stain of a dried droplet, resulting in false negatives," Wong said. "We need to think of strategies to minimize this effect."

The co-authors of the paper are postdoctoral research associate Ivan Smalyukh, graduate students Olena Zribi and John Butler, and professor Oleg D. Lavrentovich, director of the Liquid Crystal Institute at Kent State.


'"/>

Source:University of Illinois at Urbana-Champaign


Related biology news :

1. Cellular traffic backups implicated in skeletal malformations
2. Worlds largest rainforest drying experiment completes first phase
3. HIV mortality in India drops with introduction of generic antiretroviral therapy
4. Protein discovery could unlock the secret to better TB treatment
5. Tiny particles could solve billion-dollar problem
6. First atlas of key brain genes could speed research on cancer, neurological diseases
7. Discovery of key proteins shape could lead to improved bacterial pneumonia vaccine
8. A comprehensive response to HIV could prevent 10 million AIDS deaths in Africa by 2020
9. How the environment could be damaging mens reproductive health
10. Dead zone area in Gulf could be increasing, researchers say
11. Growth in biomass could put US on road to energy independence
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/26/2016)... LONDON , April 26, 2016 ... EdgeVerve Systems, a product subsidiary of Infosys (NYSE: ... a partnership to integrate the Onegini mobile security ... (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) The ... enhanced security to access and transact across channels. ...
(Date:4/14/2016)... BioCatch ™, the global ... the appointment of Eyal Goldwerger as CEO. ... Goldwerger,s leadership appointment comes at a time of significant ... of its platform at several of the world,s largest ... unique cognitive and physiological factors, is a winner of ...
(Date:3/23/2016)... 23, 2016 ... Gesichts- und Stimmerkennung mit Passwörtern     ... MESG ), ein führender Anbieter digitaler ... mit SpeechPro zusammenarbeitet, um erstmals dessen Biometrietechnologie ... die Möglichkeit angeboten, im Rahmen mobiler Apps ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... 2016 , ... Cancer experts from Austria, Hungary, Switzerland, and ... new and helpful biomarker for malignant pleural mesothelioma. Surviving Mesothelioma has just published ... , Biomarkers are components in the blood, tissue or body fluids that ...
(Date:6/27/2016)... , June 27, 2016  Liquid Biotech ... announced the funding of a Sponsored Research Agreement ... circulating tumor cells (CTCs) from cancer patients.  The ... in CTC levels correlate with clinical outcomes in ... These data will then be employed to support ...
(Date:6/24/2016)... ... 24, 2016 , ... While the majority of commercial spectrophotometers and fluorometers use ... 6000i models are higher end machines that use the more unconventional z-dimension of 20mm. ... from the bottom of the cuvette holder. , FireflySci has developed several Agilent ...
(Date:6/23/2016)... CAMBRIDGE, Mass. , June 23, 2016 /PRNewswire/ ... the development of novel compounds designed to target ... compound, napabucasin, has been granted Orphan Drug Designation ... in the treatment of gastric cancer, including gastroesophageal ... cancer stemness inhibitor designed to inhibit cancer stemness ...
Breaking Biology Technology: