Navigation Links
Researchers use multiphoton microscopy to watch chromosomes in action

Feverish fruit fly larvae, warmed in a toasty lab chamber, are giving Cornell researchers a way to watch chromosomes in action and actually see how genes are expressed in living tissue.

Using multiphoton fluorescence microscopy, a technique pioneered at Cornell by physicist Watt W. Webb, researchers have for the first time been able to watch chromosomes change their form in order to activate their genes to synthesize key proteins in fruit fly cells. The advance could be a significant step toward understanding the basic processes that underlie gene expression.

The discovery was the result of cross-disciplinary collaboration between Webb and John Lis, Cornell's Barbara McClintock Professor of Molecular Biology and Genetics. Jie Yao, who recently earned his Ph.D. at Cornell, initiated and facilitated the work.

"This technology will revolutionize the way we see gene expression in organisms," said Lis. "We're watching transcription in real time in living cells."

The research was described in the Aug. 31 issue of the journal Nature.

The team's experiments focused on gene regulatory mechanisms: specifically, what happens in a cell's nucleus when an external stimulus (heat) prompts specific genes to activate, and how those activated genes direct the production of proteins, which protect the fly against the stress of heating.

"Whenever a cell is stressed -- bingo, it will produce proteins that will help the cell resist stress," said Webb, Cornell professor of applied physics and the S.B. Eckert Professor in Engineering. The process is triggered by a molecule called heat shock factor (HSF), which interacts with genes to cue the synthesis of new proteins. But this well-known process had never been seen in living cells.

Yao used multiphoton microscopy (MPM) to image living salivary gland tissue of Drosophila (fruit flies). Unlike other methods, which lack penetrating power and can damage the specimen, MPM delivers c
'"/>

Source:Cornell University News Service


Page: 1 2 3

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
(Date:9/15/2014)... on Earth for the first 500 million years after ... present day, complete with oceans, continents and active crustal ... eon, called the Hadean, has gained substantial new support ... formed more than 4 billion years ago with those ... a possible geological analog for early Earth. , The ...
(Date:9/15/2014)... are hallmarks of the cheetah: The big predator is the ... mph in just a few seconds. As it ramps up ... bounding until it reaches a full gallop. , Now MIT ... implemented in a robotic cheetah a sleek, four-legged assemblage ... much as its feline counterpart. The team recently took the ...
(Date:9/15/2014)... who transplanted combinations of wild, domesticated, and ... to new environments found that within 5 ... foreign genes from wild populations that hybridized ... Applications study provides evidence that natural ... that hybridized populations experience as a result ...
Breaking Biology News(10 mins):Early Earth less hellish than previously thought 2Early Earth less hellish than previously thought 3Run, cheetah, run 2Run, cheetah, run 3
... how the brain controls breathing is fundamental to the ... an automatic rhythmic process that persists without conscious effort ... that has intrigued many scientists for well over 100 ... rhythm throughout life? , Experimental Physiology editor Julian Paton ...
... sudden changes in water quality, creation of coastal "dead ... climate are just some of the potential consequences of ... new United Nations-backed report launched today. , Humans have ... 50 years than in any other period, some 60 ...
... can survive an otherwise lethal dose of vaccinia virus, ... the National Institute of Allergy and Infectious Diseases (NIAID), ... say the investigators, suggest that Gleevec or similar drugs ... smallpox vaccine. The classic smallpox vaccine is made from ...
Cached Biology News:How exactly does the brain control breathing? 2UN: World in big ecological mess 2UN: World in big ecological mess 3Cancer drug slows poxvirus in mice 2Cancer drug slows poxvirus in mice 3
(Date:9/16/2014)... 16, 2014  LABSCO, the leading specialty sales ... hospitals, physician office laboratories and alternate healthcare settings ... 2014, it will serve as Siemens Healthcare Diagnostics, ... and as a semi-exclusive sales distributor in specified ... United States . LABSCO will be ...
(Date:9/16/2014)... 2014 According to Jeff Howell, Partner ... ITRA Global, new development in Downtown Toronto is expanding ... approving another 755 storeys of new development last week ... will be dominating the Toronto skyline for the foreseeable ... unquenchable thirst for new development. , As the Toronto ...
(Date:9/15/2014)... SYDNEY , Sept. 15, 2014 ... focused on revolutionizing the treatment of cancer through ... cancer cells, announced that, together with the Asbestos ... 2014 Cancer Institute New South Wales (NSW) Premier,s ... other four recipients are the hospitals that will ...
(Date:9/15/2014)... Northeastern University has developed a novel method for ... of nanocarbon structures in carbon nanotube arrays. The ... allow the researchers to tailor the physical properties ... from electronic devices to CNT-reinforced composite materials found ... Their findings were published in the journal ...
Breaking Biology Technology:LABSCO Secures Alliance With Siemens Healthcare Diagnostics 2LABSCO Secures Alliance With Siemens Healthcare Diagnostics 3ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3EnGeneIC Named a Recipient of the Cancer Institute NSW Premier's Award for Excellence in Translational Cancer Research 2EnGeneIC Named a Recipient of the Cancer Institute NSW Premier's Award for Excellence in Translational Cancer Research 3Northeastern University Researchers Develop Novel Method for Working with Nanotubes 2
... France, April 23 , - ... Essilor International, the world leader in ophthalmic optics, today,announced ... 31, 2009, representing a reported 10.3% increase on the first ... the period. , Despite contracting demand ...
... RICHMOND, Calif., April 22 Sangamo BioSciences, Inc. (Nasdaq: ... will release its first quarter 2009 financial results on ... press release will be followed by a conference call ... the public via telephone and webcast. During the conference ...
... to Discuss Results to Be Held at 9:00 A.M. - ... VPHM ) first quarter financial results for 2009 are ... the open of the U.S. financial markets.The company will host ... Eastern Time on the same day. During the conference ...
Cached Biology Technology:Essilor : 2009 First Quarter Report 2Essilor : 2009 First Quarter Report 3Essilor : 2009 First Quarter Report 4Sangamo BioSciences Announces First Quarter 2009 Conference Call and Webcast 2Sangamo BioSciences Announces First Quarter 2009 Conference Call and Webcast 3ViroPharma to Release 2009 First Quarter Financial Results on April 29, 2009 2
Sheep polyclonal to Melatonin ( Abpromise for all tested applications). Antigen: Melatonin Tg conjugate....
This antibody detects cdc42 on SDS-PAGE immunoblots....
Goat polyclonal to DDDDK tag (FITC) ( Abpromise for all tested applications)....
Rabbit polyclonal to TRPM8 (rating: ****) ( Abpromise for all tested applications). entrezGeneID: 79054 SwissProtID: Q3YFM7...
Biology Products: