Navigation Links
Researchers use mass spectrometry to detect norovirus particles

Scientists have used mass spectrometry for decades to determine the chemical composition of samples but rarely has it been used to identify viruses, and never in complex environmental samples.

Researchers at the Johns Hopkins Bloomberg School of Public Health recently demonstrated that proteomic mass spectrometry has the potential to be applied for this purpose. Using a two-step process, researchers successfully separated, purified and concentrated a norovirus surrogate from a clinical sample within a few hours. Nanospray mass spectrometry was used to demonstrate the feasibility of detecting norovirus particles in the purified concentrates.

Human norovirus is responsible for an estimated 23 million cases of gastrointestinal illness in the United States each year. This pathogen is a particular problem aboard cruise ships. The researchers believe that their mass spectrometric method could potentially be used for biodefense and public health preparedness as a tool for rapidly detecting norovirus--a category B bioterrorism agent--and other viral public health threats. The study is published in the April 2006 edition of Applied and Environmental Microbiology.

In simplified terms, mass spectrometry is essentially a scale for weighing molecules. A laser turns a sample into ionized particles, which are then accelerated in a vacuum toward a detector. The time lapsed prior to registering on the detector helps researchers determine the mass--or weight--of the particles. By targeting characteristic particles, or peptides, belonging to the viral coat protein, the virus can be positively identified by matching the results to entries in genetic databases.

In the Hopkins study, the researchers analyzed a stool sample treated with virus-like particles, which closely resemble norovirus but are noninfectious. Using mass spectrometry, the researchers were able to detect the norovirus capsid protein down to levels typically found in clinical specimens fro
'"/>

Source:Johns Hopkins University Bloomberg School of Public Health


Page: 1 2

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Researchers use mass spectrometry detect norovirus particles

(Date:7/28/2014)... Norwegian explorer Roald Amundsen reached the South Pole in ... arrived long before any human. , Using data from ... the Antarctic continent, including the South Pole, a group ... (DRI) in Reno, Nevada, created the most accurate and ... southernmost continent. The new record, described in an article ...
(Date:7/28/2014)... team of researchers led by the University of Arizona ... The genetic information will enhance scientists, and agriculturalists, understanding ... as enable the development of new rice varieties that ... to help solve global hunger challenges. , The paper, ... ) and evidence for independent domestication," was published online ...
(Date:7/28/2014)... United States have a circulatory problem of the legs ... may even require surgery in serious cases. This disease ... turn, limb amputation. , At The University of Texas ... tested a non-surgical preventative treatment in a mouse model ... blood circulation. Their proof-of-concept study appears in the ...
Breaking Biology News(10 mins):Lead pollution beat explorers to South Pole, persists today 2Lead pollution beat explorers to South Pole, persists today 3Generating a genome to feed the world: UA-led team sequences African rice 2Generating a genome to feed the world: UA-led team sequences African rice 3Generating a genome to feed the world: UA-led team sequences African rice 4Scientists discover genetic switch that can prevent peripheral vascular disease in mice 2
... Health have discovered specific molecular and signaling events by ... showed that low levels of Vitamin D, comparable to ... the inflammatory cascade, while levels considered adequate did inhibit ... 1, 2011, issue of The Journal of Immunology ...
... an outbreak of airborne H5N1 influenza in humans needs to ... can safely continue H5N1 research and share the results among ... mBio , the online open-access journal of the American Society ... been at the center of heated discussions in science and ...
... are frequently reminded of the complex challenges Africa faces ... the sub-Saharan region. Dealing with hunger and famine ... and needs a global effort. Overcoming these challenges in ... and requires increased efforts in research and development. ...
Cached Biology News:Researchers discover how vitamin D inhibits inflammation 2Opinion: H5N1 flu is just as dangerous as feared, now requires action 2Opinion: H5N1 flu is just as dangerous as feared, now requires action 3Cutting-edge science creating solutions for African agriculture 2
(Date:7/28/2014)... SAN DIEGO , July 28, 2014 ... algae cultivation technologies, today announced the appointment of biotechnology ... member of the board of directors. Levine replaces Cynthia ... effective immediately. She will remain chairman of the company,s ... leadership on Sapphire Energy,s mission to deliver commercial scale ...
(Date:7/28/2014)... 2014 The Workgroup for Electronic Data ... use of health IT to create efficiencies in healthcare ... (HPID) Workgroup has developed an issue brief titled, ... Payer?” The HPID Workgroup, a part of WEDI’s Strategic ... for Medicare and Medicaid Services (CMS) to identify common ...
(Date:7/28/2014)... 28, 2014 “2014 Deep ... Industry” is a professional and in-depth research ... The report introduces Lubricant basic information, including ... structure and industry overview. This research covers ... market as well as global industry analysis ...
(Date:7/28/2014)... 2014 According to a new ... "Regenerative Medicine (Bone and Joint) Market (By Technology ... By Applications - Bone Graft Substitutes, Osteoarticular Diseases, ... Industry Analysis, Size, Share, Growth, Trends and Forecast, ... and joint) market was valued at USD 2.6 ...
Breaking Biology Technology:Sapphire Energy Announces New CEO James Levine 2Sapphire Energy Announces New CEO James Levine 3WEDI’s HPID Workgroup Announces Release of Issue Brief: “What is the Difference Between a Health Plan and Payer?” 2Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 2Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 3Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 4Regenerative Medicine Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2013 - 2019 2Regenerative Medicine Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2013 - 2019 3Regenerative Medicine Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2013 - 2019 4
... Calif., Oct. 27 Beckman,Coulter, Inc. (NYSE: ... and marketer,of products that simplify, automate, and ... Garrett, Beckman Coulter,s chairman,president and chief executive ... Conference on Tuesday, November 4, 2008 at ...
... 27 Dr. Annie De Groot, CEO of,EpiVax, is ... about,the activation of natural regulatory T cells by IgG ... were published in the,October 8 issue of the prestigious ... Hematology., In an accompanying editorial, Rachel Caspi of ...
... Facility, NEW YORK, Oct. 27 NeoStem, Inc. ... pre-disease collection,processing and long-term storage of adult stem cells ... on a private placement financing,in the amount of $250,000. ... stem cell collection network in the New York City ...
Cached Biology Technology:Publication on 'Tregitopes' in Blood Journal of October 2008 2NeoStem Secures $250,000 in an Above Market Financing and Expands Its Relationship With New England Cryogenic Center, Inc. 2NeoStem Secures $250,000 in an Above Market Financing and Expands Its Relationship With New England Cryogenic Center, Inc. 3
Guinea Pig Complement...
Collected from 8-12 week-old New Zealand or Californian rabbits....
Enables rapid purification of double-stranded DNA fragments = 200 bp, including, for example, PCR reaction products, DNA fragments obtained in restriction enzyme digests, or double-stranded cDNA...
5 minutes from gel band to purified DNA...
Biology Products: