Navigation Links
Researchers show key protein necessary for normal development of red blood cells

Virginia Commonwealth University researchers studying hemoglobin genes, mutations of which play a role in genetic blood disorders like sickle cell anemia and beta-thalassemia, have shown in studies with mice that the KLF2 protein is crucial for making young red blood cells.

The findings may point researchers to future gene therapies for patients with sickle cell anemia and beta-thalassemia.

In the October issue of Blood, the journal of the American Association for Hematology, researchers demonstrated that a protein called KLF2 regulates the production of embryonic globin genes and the maturation and stability of embryonic red blood cells in a mouse model. Researchers observed that KLF2 is responsible for controlling and "turning on" the embryonic globin gene.

"Understanding how genes are turned on and off, and the switch from the embryonic globin gene to the adult beta-globin gene has clinical relevance to treatment of sickle cell anemia and beta-thalassemia," said Joyce A. Lloyd, Ph.D., associate professor of Human Genetics at VCU, and corresponding author for this study.

"Our findings are significant for future treatment of these blood disorders, potentially using gene therapies and other novel strategies," she said. In gene therapy, a normal DNA is inserted into cells to correct a genetic defect. To correct the defect or mutation, a gene may be replaced, altered or supplemented.

According to Lloyd, the production of blood cells involves a complex differentiation pathway that involves the interaction of many molecular players and proteins.

In humans, there are four globin genes clustered on chromosome 11 in the order in which they are "turned on" or expressed. These genes include the epsilon-globin gene, two gamma-globin genes and the beta-globin gene. Lloyd said that during fetal development, the embryonic epsilon-globin gene is active first, followed by the gamma-globin genes, and finally the adult form, beta-glo bin takes control following birth.

Lloyd and Priyadarshi Basu, Ph.D., lead investigator at VCU, and the research team compared mice that were missing the gene for KLF2 to normal mice. They found that the KLF2-deficient mice produced embryonic red blood cells that appeared abnormal, were more likely to undergo cell death, and produced significantly lower amounts of globin mRNA than those found in normal mice. Globin mRNA is a key player in gene expression that helps translate the DNA's genetic code.

Lloyd and her colleagues identified that the role of KLF2 for the embryonic epsilon-globin genes is analogous to that of a protein called EKLF. EKLF plays a central role in the developmental regulation of the adult beta-globin gene, and is essential for the maturation and stability of adult red blood cells. Researchers believe that the roles of EKLF and KLF2 may partially overlap in controlling human embryonic and fetal globin gene expression.

This research was supported by a grant from the National Institutes of Health.

Lloyd collaborated with colleagues in the VCU Department of Human Genetics, and the VCU Department of Anatomy and Neurobiology; the Department of Molecular Genetics, Biochemistry and Microbiology at the University of Cincinnati; and the Department of Medicine at the University of California-San Francisco.


'"/>

Source:Virginia Commonwealth University


Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
(Date:4/5/2017)... April 4, 2017 KEY FINDINGS ... expand at a CAGR of 25.76% during the forecast ... the primary factor for the growth of the stem ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem cell ... application, and geography. The stem cell market of the ...
(Date:4/3/2017)... April 3, 2017  Data captured by ... platform, detected a statistically significant association between ... to treatment and objective response of cancer ... to predict whether cancer patients will respond ... as well as to improve both pre-infusion potency ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... announced today that they have entered into a multiyear collaboration to identify and ... researchers with additional tools for gene editing across all applications. , Under the ...
(Date:10/12/2017)... ... October 12, 2017 , ... BioMedGPS announces expanded coverage of ... newest module, US Hemostats & Sealants. , SmartTRAK’s US Market for Hemostats and ... synthetic sealants and biologic sealants used in surgical applications. BioMedGPS estimates the market ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... first-ever genomics analysis platform specifically designed for life science researchers to analyze ... pioneering researcher Rosalind Franklin, who made a major contribution to the discovery ...
(Date:10/11/2017)... ... 11, 2017 , ... The CRISPR-Cas9 system has ... and avoiding the use of exogenous expression plasmids. The simplicity of programming this ... gain-of-function studies. , This complement to loss-of-function studies, such as with RNAi ...
Breaking Biology Technology: