Navigation Links
Researchers report technique for freezing and preserving genetically enhanced pig embryos

Researchers led by a University of Missouri-Columbia professor of reproductive biotechnology have reported success in freezing and preserving swine embryos that were created by in vitro techniques and that carried modified genetic material. After thawing and transfer to a surrogate mother, some of the embryos went on to produce live piglets with new genetic traits.

In a paper posted today (May 3, 2006) on the Web site of Biology of Reproduction--Papers in Press, a team headed by Dr. Randall Prather, co-director of the National Swine Resource and Research Center, note that their technique could prove valuable in allowing genetically enhanced swine to be transported as embryos across the country and throughout the world.

Because many embryos are needed to produce a successful pregnancy in surrogate female pigs, this new procedure will enable workers to collect an adequate number of altered embryos and store them until they can be transplanted to a surrogate.

Swine embryos have been difficult to freeze and preserve because of their sensitivity to cold due to a high level of lipids in the cells of the embryos. It was expected that it would be even more difficult to freeze and preserve swine embryos that had been produced by in vitro methods.

Dr. Prather's team overcame the obstacles to freezing swine embryos by first removing lipids from unfertilized eggs before fertilizing them with muscle cells from a male pig containing modified genetic material. The resulting embryos were then frozen at the blastocyst stage.

Upon thawing, embryos were transferred to surrogate female swine. Embryos placed in the oviducts of two surrogates led to pregnancies that produced two piglets in one litter and eight piglets in another.

Testing confirmed that the piglets carried the modified genetic material and that the new gene had altered the fatty-acid content in tissues from the piglets.

With ten piglets from 163 frozen and preserved embryos, the researchers feel that their procedure appears to work well, although further studies will be needed to refine the technique.

Dr. Prather and his team expect that freezing and preserving swine embryos will enable wide dissemination of swine with genetic traits that are commercially valuable and also important for biomedical research involving this increasingly relevant animal model for human disorders.


Source:Society for the Study of Reproduction

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:

(Date:6/22/2016)... ANGELES , June 22, 2016 /PRNewswire/ ... identity management and verification solutions, has partnered ... edge software solutions for Visitor Management, Self-Service ... provides products that add functional enhancements ... partnership provides corporations and venues with an ...
(Date:6/15/2016)... New York , June 15, 2016 /PRNewswire/ ... a new market report titled "Gesture Recognition Market by ... and Forecast, 2016 - 2024". According to the report, ... USD 11.60 billion in 2015 and is estimated ... reach USD 48.56 billion by 2024.  ...
(Date:6/2/2016)... 2, 2016 The Department of Transport ... the 44 million US Dollar project, for the , ... including Personalization, Enrolment, and IT Infrastructure , to ... production and implementation of Identity Management Solutions. Numerous renowned international ... Decatur was selected for the most compliant ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... ... on what they believe could be a new and helpful biomarker for malignant ... Click here to read it now. , Biomarkers are components in ...
(Date:6/27/2016)... 2016  Liquid Biotech USA ... a Sponsored Research Agreement with The University of ... from cancer patients.  The funding will be used ... with clinical outcomes in cancer patients undergoing a ... be employed to support the design of a ...
(Date:6/24/2016)... ... 2016 , ... While the majority of commercial spectrophotometers and fluorometers use the ... models are higher end machines that use the more unconventional z-dimension of 20mm. ... the bottom of the cuvette holder. , FireflySci has developed several Agilent flow ...
(Date:6/23/2016)... June 23, 2016   Boston Biomedical , ... compounds designed to target cancer stemness pathways, announced ... granted Orphan Drug Designation from the U.S. Food ... gastric cancer, including gastroesophageal junction (GEJ) cancer. Napabucasin ... to inhibit cancer stemness pathways by targeting STAT3, ...
Breaking Biology Technology: