Navigation Links
Researchers develop new method for facile identification of proteins in bacterial cells

Researchers at the Johns Hopkins Bloomberg School of Public Health have developed a new method for identifying specific proteins in whole cell extracts of microorganisms using traditional peptide mass fingerprinting (PMF). The key to the new method, according to the researchers, is a "shortcut" for preparing samples that makes PMF faster and more economical. By reducing the cost of protein identification, they believe PMF can become an economical tool for monitoring and evaluating the effectiveness of microorganisms used in environmental cleanup. The researchers used a dioxin-eating organism to demonstrate the capabilities of their methodology, which they described in an article published in the May 2005 edition of Applied and Environmental Microbiology.

PMF typically involves elaborate sample preparation. A protein mixture is spread across a gel and separated into individual proteins, which are scooped out of the gel and cut with protein scissors into predictable, small pieces called peptides. The samples are then analyzed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), which identifies protein fragments based on the time they need to travel a defined distance when being accelerated in a vacuum.

In their study, Rolf U. Halden, PhD, PE, assistant professor in the Department of Environmental Health Sciences Bloomberg School of Public Health and his colleagues demonstrate how PMF and mass spectrometry are used to identify a unique dioxin-degrading enzyme in a soup of hundreds of cell proteins. The technique avoids elaborate conventional sample preparation steps by coaxing the cells into mass production of the protein the researchers wish to analyze.

"Finding a specific target in a mixture of hundreds of proteins can be likened to finding the proverbial needle in the haystack; this task can be performed much faster and more economically if you have more needles--and that's exactly what our method
'"/>

Source:Johns Hopkins University Bloomberg School of Public Health


Page: 1 2

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain

Post Your Comments:
(Date:11/23/2009)... WI, November 16, 2009 -- A USDOE and USDA study c...dle cropland, and cropland pasture could be conver...al grasses, such as switchgrass, from which biomas...k. Economically viable production of a perennial g... of biomass are removed annually is expected to re...
(Date:11/23/2009)..., N.H. University of New Hampshire microbiologist...115 to advance understanding of the actinorhizal p...nrich nutrient-poor and contaminated soils. , T...awarded Tisa, a professor of molecular, cellular a...mbiotic relationship between the bacteria Frankia ...
(Date:11/23/2009)...sity of Southern California biomedical engineer an... a new tool to help clinicians distinguish cardiac...nic problems manageable with drugs and lifestyle c...serted into the arteries feeding the heart, offer ...f these blood vessels, often revealing deposits of...
Breaking Biology News(10 mins):Switchgrass produces biomass efficiently 2UNH prof. receives nearly $500,000 to research environmentally significant plants 2Stable plaque or heart attack plaque? USC researcher builds new sensor to tell which is which 2Wellmont Health System Awards Contract to MedQuist for Clinical Documentation Services 55426 1Wellmont Health System Awards Contract to MedQuist for Clinical Documentation Services 55426 2Wellmont Health System Awards Contract to MedQuist for Clinical Documentation Services 55426 3September is Vision Safety Month at the Vision Center CHLA 3A Sports Eye Injuries a Leading Cause of Blindness in Children 55422 1September is Vision Safety Month at the Vision Center CHLA 3A Sports Eye Injuries a Leading Cause of Blindness in Children 55422 2September is Vision Safety Month at the Vision Center CHLA 3A Sports Eye Injuries a Leading Cause of Blindness in Children 55422 3Low Carb Diet Referenced in BIDMC Study Not Related to Atkins 55420 1Low Carb Diet Referenced in BIDMC Study Not Related to Atkins 55420 2Low Carb Diet Referenced in BIDMC Study Not Related to Atkins 55420 3Low Carb Diet Referenced in BIDMC Study Not Related to Atkins 55420 4Low Carb Diet Referenced in BIDMC Study Not Related to Atkins 55420 5
...0 physicians, scientists and allied health profess...ventional Radiology,s 34th Annual Scientific Meeti...Scientific press conferences are scheduled for 9-1...rch 10 in Room 15A. , The Annual Scientific Meeti...s and posters covering the newest trends in interv...
...detail carbon dioxide emissions from fossil fuel c...Earth platform. The maps, funded by NASA and the U...merican Carbon Program, can display fossil fuel em...ype. , A science team led by researchers at Purdu...even primary data sets, including imagery of Earth...
...ebruary 19, 2009)The Gulf Coast Community Foundati...UM) $250,000 toward expansion of research and educ... and ecological preserve, Little Salt Spring (LSS)...e is of enormous archeological and anthropological...vation of ancient organic material. , The gift wa...
Other Biology News:Register now for the Society of Interventional Radiology's 34th Annual Scientific Meeting 2NASA-funded carbon dioxide map of US released on Google Earth 2Little Salt Spring underwater archeological site in Florida receives new funding 2Little Salt Spring underwater archeological site in Florida receives new funding 3
(Date:11/24/2009)...E,Nov.24/PRNewswire/--BlueMarbleEnergyCorp.announc...Authority(OPDA)wererecentlyawarded$2millionbyWashi...ERB)inaprivate/publicpartnershiptoconstructthestat...unty,WA. ,, "Thisinvestmentwillsignificantlyspe...njobstoLincolnCounty,"saidBlueMarbleEnergyCEOKelly...
(Date:11/24/2009)...,November24/PRNewswire-FirstCall/--Shireplc(LSE:SH...icalcompany,today,announcedthatithassubmittedaMAAt...hecompany,senzymereplacementtherapyindevelopment,f...marketing,applicationforvelaglucerasealfathathasbe...tesandCanada. , Basedonaglobalsupplyshortageo...
(Date:11/24/2009)... Program Connects Customers with Partne...ted with DNA Genotek Products , ...A Genotek , a leading provider of products for bio...ration, today announced its new global partner pro...for DNA Genotek,s partner community and is designe...
(Date:11/23/2009)...ll. You can think of it as origami very high-tec...inois have developed a technique for fabricating t...tures from thin films by coupling photolithography...teractions. , The films, only a few microns thick...e with thicker pieces of the same material. , "Th...
Breaking Biology Technology:Blue Marble Energy, OPDA Awarded $2M by WA's Community Economic Revitalization Board 2Shire Submits European Marketing Authorization Application (MAA) for velaglucerase alfa for the Treatment of Type 1 Gaucher Disease 2Shire Submits European Marketing Authorization Application (MAA) for velaglucerase alfa for the Treatment of Type 1 Gaucher Disease 3DNA Genotek Launches Global Partner Program 2DNA Genotek Launches Global Partner Program 3Water droplets direct self-assembly process in thin-film materials 2
...iv University a network of tiny sensors as small ... most determined intruder. Scattered outdoors on ... floor of a bank, the dewdrops are a completely ne...ecuring wide swathes of property. , Prof. Yoram S...neering team drew upon the space-age science of mo...
...Sunesis Pharmaceuticals, Inc. (Nasdaq: SNSS ) re...l 14, 2009, from the Listing Qualifications Depart...any that it does not comply with the $10.0 million...inued listing on The NASDAQ Global Market set fort...,s determination was based on a review of the Comp...
...cal development solutions in North America, Europe... ClinPharm (SIRO), a leading multinational Contra...e signing of a strategic alliance with Advanced Cl...gton, NJ, USA, a service provider in oncology drug...h America. This alliance will enable SIRO to expa...
Other Biology Technology:Intruder alert: Tel Aviv University's 'Smart Dew' will find you! 2Sunesis Pharmaceuticals Receives NASDAQ Notification 2Sunesis Pharmaceuticals Receives NASDAQ Notification 3SIRO ClinPharm and Advanced Clinical Trial Solutions Ink Strategic Alliance 2
Rabbit Anti-Rat Heme Oxygenase-1 (2-23) Polyclonal Antibody, Unconjugated from Calbiochem
Vitality pIRES-hrGFP-1 Vector from Stratagene
1,2-Distearoyl-sn-Glycero-3-Phosphoethanolamine-N- [Carboxy(Polyethylene Glycol)2000] Ammonium Salt from Avanti Polar Lipids Inc.
HyQ SFX-Insect MP (1 x 5 L powder) from Hyclone
Biology Products: