Navigation Links
Researchers Find New Technique To Identify Fetal Genetic Material From Amniotic Fluid

A preliminary report suggests that cell-free fetal messenger RNA (ribonucleic acid) can be extracted from amniotic fluid (fluid around the fetus), and then be analyzed to study gene expression changes that may reflect the well-being of the fetus, according to a paper in the February 16 issue of JAMA, a theme issue on medical applications of biotechnology.

In background information in the article, the authors write that fetal monitoring in the womb is now "limited to noninvasive methods such as measurement of uterine size or anatomic evaluation by fetal sonography. In addition, genetic analysis can be performed on amniotic fluid components ? They add that the "cell-free component of the amniotic fluid is discarded after these analyses and is therefore available for research and future clinical applications."

Paige B. Larrabee, M.D., from Tufts-New England Medical Center, Boston, and colleagues analyzed four samples of cell-free amniotic fluid from pregnant women between 20 and 32 weeks' gestation and who had certain conditions that required procedures to reduce an excessive amount of amniotic fluid. The control group in this study was 6 pooled amniotic fluid samples from women at 17 weeks' gestation who were undergoing amniocentesis (removal of amniotic fluid to test for hereditary diseases and congenital defects in the fetus). "After extraction from the normally discarded fraction of amniotic fluid, RNA was amplified twice, labeled, and analyzed using gene expression microarrays." The researchers were able to study the expression of developmental transcripts, such as for certain proteins.

"Preliminary analysis suggests that gene expression changes can be detected in fetuses of different sexes, gestational age, and disease status," the authors report. "Cell-free mRNA in amniotic fluid appears to originate from the fetus and not the placenta." In conclusion the authors write: "The intriguing gene expression differences observed suggest that this technology could facilitate the advancement of human developmental research, as well as the cultivation of new biomarkers for assessment of the living fetus."

###

(JAMA. 2005; 293:836-842. Available post-embargo at JAMA.com)

Editor's Note: This study was supported by a grant from the National Institutes of Health. Co-authors Drs. Larrabee, Johnson, and Bianchi have filed for patents for the methodology described in this article.


'"/>

Source:Journal Of The American Medical Association


Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/15/2016)... York , June 15, 2016 ... new market report titled "Gesture Recognition Market by Application ... Forecast, 2016 - 2024". According to the report, the  ... 11.60 billion in 2015 and is estimated to ... USD 48.56 billion by 2024.  Increasing ...
(Date:6/7/2016)... , June 7, 2016  Syngrafii Inc. ... a business relationship that includes integrating Syngrafii,s patented ... branch project. This collaboration will result in greater ... the credit union, while maintaining existing document workflow ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/2/2016)... --  The Weather Company , an IBM Business (NYSE: ... in which consumers will be able to interact with IBM ... voice or text and receive relevant information about the product ... have long sought an advertising solution that can create a ... and valuable; and can scale across millions of interactions and ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... (PRWEB) , ... December 01, 2016 , ... ... congratulate long-term client Nanowear on their recent FDA Class II 510(k) clearance for ... a significant hurdle in commercializing remote cardiac monitoring devices that rely on cloth-based ...
(Date:12/2/2016)...   SurePure, Inc. (OTCQB: SURP) a global ... has concluded an agreement with Tamarack Biotics under which ... to acquire units of the Company,s patented photopurification technology ... Concurrently with the option, SurePure has ... will seek regulatory approvals in the United ...
(Date:11/30/2016)... ... November 30, 2016 , ... BEI ... Coil Actuator with a flexure design that ensures high alignment accuracy by preventing ... and is ideally suited where extreme precision is required, such as in medical ...
(Date:11/30/2016)... 2016 /PRNewswire/ - Portage Biotech Inc. ("Portage" or "the ... excited to announce the formation of EyGen, Ltd. ... ophthalmology assets through proof of concept. EyGen,s lead ... Portage Pharmaceuticals Limited and being developed for topical ... anterior segment diseases. This agent has the potential ...
Breaking Biology Technology: