Navigation Links
Researchers Find New Technique To Identify Fetal Genetic Material From Amniotic Fluid

A preliminary report suggests that cell-free fetal messenger RNA (ribonucleic acid) can be extracted from amniotic fluid (fluid around the fetus), and then be analyzed to study gene expression changes that may reflect the well-being of the fetus, according to a paper in the February 16 issue of JAMA, a theme issue on medical applications of biotechnology.

In background information in the article, the authors write that fetal monitoring in the womb is now "limited to noninvasive methods such as measurement of uterine size or anatomic evaluation by fetal sonography. In addition, genetic analysis can be performed on amniotic fluid components ? They add that the "cell-free component of the amniotic fluid is discarded after these analyses and is therefore available for research and future clinical applications."

Paige B. Larrabee, M.D., from Tufts-New England Medical Center, Boston, and colleagues analyzed four samples of cell-free amniotic fluid from pregnant women between 20 and 32 weeks' gestation and who had certain conditions that required procedures to reduce an excessive amount of amniotic fluid. The control group in this study was 6 pooled amniotic fluid samples from women at 17 weeks' gestation who were undergoing amniocentesis (removal of amniotic fluid to test for hereditary diseases and congenital defects in the fetus). "After extraction from the normally discarded fraction of amniotic fluid, RNA was amplified twice, labeled, and analyzed using gene expression microarrays." The researchers were able to study the expression of developmental transcripts, such as for certain proteins.

"Preliminary analysis suggests that gene expression changes can be detected in fetuses of different sexes, gestational age, and disease status," the authors report. "Cell-free mRNA in amniotic fluid appears to originate from the fetus and not the placenta." In conclusion the authors write: "The intriguing gene expression differences observed suggest that this technology could facilitate the advancement of human developmental research, as well as the cultivation of new biomarkers for assessment of the living fetus."

###

(JAMA. 2005; 293:836-842. Available post-embargo at JAMA.com)

Editor's Note: This study was supported by a grant from the National Institutes of Health. Co-authors Drs. Larrabee, Johnson, and Bianchi have filed for patents for the methodology described in this article.


'"/>

Source:Journal Of The American Medical Association


Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. Researchers reveal the infectious impact of salmon farms on wild salmon
5. Researchers identify target for cancer drugs
6. Researchers discover molecule that causes secondary stroke
7. Researchers find missing genes of ancient organism
8. Researchers trace evolution to relatively simple genetic changes
9. Researchers add new tool to tumor-treatment arsenal
10. UF Researchers Map Bacterial Proteins That Cause Tooth Loss
11. VCU Researchers Identify Networks Of Genes Responding To Alcohol In The Brain
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/16/2016)... FRANCISCO , June 16, 2016 ... size is expected to reach USD 1.83 ... by Grand View Research, Inc. Technological proliferation and ... banking applications are expected to drive the market ... ) , The development of advanced ...
(Date:6/9/2016)... innovation leader in attendance control systems is proud to announce the introduction of fingerprint ... sure the right employees are actually signing in, and to even control the opening ... ... ... Photo ...
(Date:6/7/2016)... 7, 2016  Syngrafii Inc. and San Antonio ... that includes integrating Syngrafii,s patented LongPen™ eSignature "Wet" ... collaboration will result in greater convenience for SACU ... while maintaining existing document workflow and compliance requirements. ... Highlights: ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... ... will join the faculty of the University of North Carolina Kenan-Flagler Business ... strategy and entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s international ...
(Date:6/24/2016)... , June 24, 2016 Epic ... sensitively detects cancers susceptible to PARP inhibitors by ... tumor cells (CTCs). The new test has already ... therapeutics in multiple cancer types. Over ... DNA damage response pathways, including PARP, ATM, ATR, ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, ... launch of their brand, UP4™ Probiotics, into Target stores nationwide. The company, which ... to add Target to its list of well-respected retailers. This list includes such ...
(Date:6/23/2016)... June 23, 2016 Houston Methodist Willowbrook ... Cy-Fair Sports Association to serve as their official ... Houston Methodist Willowbrook will provide sponsorship support, athletic ... with association coaches, volunteers, athletes and families. ... Cy-Fair Sports Association and to bring Houston Methodist ...
Breaking Biology Technology: