Navigation Links
Recombination protein dynamics observed with single monomer resolution

Using a sensitive, single-molecule measurement technique, researchers at the University of Illinois at Urbana-Champaign have observed the life cycle of RecA, a protein that plays a major role in repairing damaged DNA.

The protein forms a filament, which grows and shrinks primarily by one monomer at a time, the researchers report in the August issue of the journal Cell.

RecA is a DNA recombination protein found in the gut bacterium E. coli. A human homolog, called Rad51, interacts with many proteins, including BRCA2, whose mutation increases susceptibility to breast and ovarian cancers. A better understanding of how these proteins function could help our understanding of cancer.

"Our measurement technique provides a way of counting the number of individual monomers bound to DNA in real time," said Taekjip Ha, a professor of physics at Illinois and a Howard Hughes Medical Institute investigator. "With that, we can determine the kinetic rates for reactions occurring at either end of the protein filament."

During the recombination process, RecA binds with DNA to form a filament that spirals around the DNA. The filament can grow in either direction, and can advance on the DNA by growing at the leading end and dissociating at the trailing end.

To study the dynamics of RecA, the researchers used a highly sensitive single-molecule fluorescence resonance energy transfer (FRET) technique that Ha and colleagues developed.

To use FRET, researchers first attach two dye molecules ?one green and one red ?to the molecule they want to study. Next, they excite the green dye with a laser. Some of the energy moves from the green dye to the red dye, depending upon the distance between them.

The researchers then measure the brightness of the two dyes simultaneously. The changing ratio of the two intensities indicates the relative movement of the two dyes, and therefore the motion of the molecule or its change in size.

The te
'"/>

Source:University of Illinois at Urbana-Champaign


Page: 1 2

Related biology news :

1. DNA Recombination and Repair—A New Twist to RecA Function
2. New, automated tool successfully classifies and relates proteins in unprecedented way
3. New binding target for oncogenic viral protein
4. Controversial drug shown to act on brain protein to cut alcohol use
5. Timing is everything: First step in protein building revealed
6. UWs Rosetta software to unlock secrets of many human proteins
7. Researchers find how protein allows insects to detect and respond to pheromones
8. Signaling protein builds bigger, better bones in mice
9. Ancient olfaction protein is shared by many bugs, offering new pest control target
10. Automatic extraction of gene/protein biological functions from biomedical text
11. Discovery of key proteins shape could lead to improved bacterial pneumonia vaccine
Post Your Comments:
(Date:11/12/2014)... BEACH GARDENS, Fla. , Nov. 12, 2014 /PRNewswire/ ... solutions, announced today that its U.are.U ® fingerprint ... retail bakery chain in Central Mexico ... in June 2014 to eliminate payroll issues caused by ... Prior to installing the U.are.U fingerprint readers, Montparnasse relied ...
(Date:11/7/2014)... Nov. 7, 2014  In conjunction with the ... Departments, The Community Foundation of the Verdugos has announced the ... JUSTICE PROJECT," a $250,000 initiative to keep the Verdugo Regional ... its first six months of existence, the Lab has processed ... for local law enforcement. "Because our number one ...
(Date:11/6/2014)... asset for a predator. Except when that predator runs so ... relative to its size, is the fastest creature on Earth. ... per second (at about five miles per hour). The fastest ... sprinting gold from the tiger beetle, a person would have ... beetle has a problem. At peak speeds, everything becomes a ...
Breaking Biology News(10 mins):Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2The tiger beetle: Too fast to see 2
... It may not be obvious, but hearing two ... to bilingualism by birth. According to new findings in ... for Psychological Science, infants born to bilingual mothers (who ... preferences than infants born to mothers speaking only one ...
... Scorpion venom is notoriously poisonous ― but it might ... painkillers like morphine, a Tel Aviv University researcher claims. ... University,s Department of Plant Sciences is investigating new ways ... found in the venom of scorpions. These compounds have ...
... (Birgitta) of Sweden that has been kept in a shrine ... conducted at Uppsala University reveals that the two skulls, believed ... is not from maternally related individuals. Furthermore, dating show that ... and Catherine lived. The findings are published in the journal ...
Cached Biology News:Bilingual babies: The roots of bilingualism in newborns 2Pinch away the pain 2Pinch away the pain 3The putative skull of St. Bridget can be questioned 2
(Date:11/26/2014)... November 26, 2014 The North ... segments the market for detailed analysis of the ... market to reach $616.3 million by 2018, growing ... 2018. , Browse through the TOC of the ... get an idea of the in-depth analysis and ...
(Date:11/26/2014)... The Alliance for Safe Biologic ... European physicians at the "1 ST EuropaBio ... at the Spanish Ministry of Health, Social Services ... the Spanish Bioindustry Association (ASEBIO), included regulators from ... oncology and rheumatology societies, representatives from a hospital ...
(Date:11/26/2014)... (PRWEB) November 25, 2014 Whitehouse ... officially joined the Morris County (New Jersey) Chamber ... is a leading business organization in the North ... to further engage some key clients in the ... in the geographic area. Membership enables Whitehouse Laboratories ...
(Date:11/26/2014)... 25, 2014 The Pittcon marketing department ... conference and exposition mobile app, Pittcon ... in the App Store for iOS and Android devices ... tool before, during and after the event. It acts ... exhibiting companies, technical sessions, Conferee Networking sessions and short ...
Breaking Biology Technology:The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 2The North America Thermal Protective Clothing Market is estimated to grow up to $616.3 million by 2018 - Report by MicroMarket Monitor 3ASBM Presents European Physicians Survey at Spanish Ministry of Health 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Pittcon Releases 2015 Mobile App 2
... N.C., Feb. 6 PPD, Inc. (Nasdaq:,PPDI) today announced ... independent contract research organization, or CRO, based in,Smolensk, Russia. ... in both Russia and Ukraine., One of the ... the,implementation of good clinical practice (GCP) in the former ...
... Idenix,Pharmaceuticals, Inc. (Nasdaq: IDIX ) announced today ... upcoming BIO CEO & Investor,Conference on Tuesday, February 12, ... Center/North Room at the Waldorf-Astoria Hotel in New York, ... presentation can be accessed,under "Calendar of Events" in the ...
... Further Solidifies Netsmart as Leader in Behavioral Healthcare Solutions for ... ... GREAT RIVER, N.Y., Feb. 6 Netsmart Technologies, Inc.,a leading provider ... announced it has signed a contract to,provide comprehensive licensed software and ...
Cached Biology Technology:PPD to Acquire InnoPharm Ltd. 2PPD to Acquire InnoPharm Ltd. 3Netsmart Technologies Extends Relationship with Manatee Glens for Enterprise Behavioral Health Software Solutions 2Netsmart Technologies Extends Relationship with Manatee Glens for Enterprise Behavioral Health Software Solutions 3
Request Info...
2-min, single-step add-mix-measure assay for luciferase expression and HTS in mammalian cells. Linear detection range: 2 fg to 46 ng luciferase....
Trop-2 Purified Anti-Human clone 162-46, Isotype Mouse IgG 1 , 100 µg Consult technical datasheet for details....
... Adapted to Sf-900 ... were isolated from Spodoptera ... insect cells (1). The ... to serum-free suspension culture ...
Biology Products: