Navigation Links
Recombinant DNA technology may enable oral, rather than injectable, delivery of protein drugs

o receptors on the intestinal epithelial cells and be transported across the GI tract to the blood stream," Shen says. "Humans naturally produce transferrin to move iron through the blood to the liver, spleen and bone marrow. It has been used to carry some protein-based drugs in a piggy-back fashion to their necessary site of action."

The next step, Shen explains, was isolating the genetic code for G-CSF, a protein factor that stimulates white blood cell production in the body. G-CSF is used to make Neupogen(r) and Neulasta(r) - injectable drugs that work to keep an individual's white blood cell count at normal levels during chemotherapy.

After that, the researchers needed to bring transferrin and G-CSF together, a process that is detailed in the PNAS paper. "Through recombinant DNA technology, we combined the genetic codes for both human transferrin and G-CSF to create a new recombinant DNA, which, when expressed in a cell, will produce a protein with half transferrin and half G-CSF," says Shen. "We refer to this method as recombinant fusion protein technology."

Recombinant DNA technology utilizes a series of procedures to join (recombine) segments of two or more different DNA molecules. When put into cell culture, a recombinant DNA molecule multiplies itself to form a colony of daughter cells that secretes the desired protein. These cells become "factories" for the production of the protein coded for by the inserted DNA.

As it turned out, this was the case as well for the transferrin/G-CSF combination.

"Our recombinant fusion protein was administered orally, and when tested in mice, increased the white blood cell count for three days, whereas the injectable agents only maintain effectiveness for one day," Shen says. "We have finally produced an orally-administered protein with a desirable therapeutic activity. This technique can be used to create orally-administered versions of other currently injectable protein drugs such a
'"/>

Source:Eurekalert


Page: 1 2 3

Related biology news :

1. Live Recombinant Adenovirus Vaccine Technique Explored
2. Novel technology detects human DNA mutations
3. Novel antiviral technology inhibits RSV infection in mice
4. The BIOS Initiative - open source biotechnology is born
5. Revolutionary nanotechnology illuminates brain cells at work
6. Study: homemade gene expression technology unreliable
7. First technology to remove prions that cause vCJD from blood launched
8. Integration of Agilents MS technology, Proteome Systems software to help scientists in proteomics research
9. Agilent Technologies new genome analysis technology set to accelerate Australia fight against mesothelioma
10. First production of human monoclonal antibodies in chicken eggs published in Nature Biotechnology
11. Vietnam war technology could aid elephant conservation
Post Your Comments:
(Date:12/17/2014)... 2014  Automation is fundamentally transforming the travel ... at international borders. Over the past decade, ePassports, ... veteran travelers to self process through border control ... at an increasing number of airports, seaports, and ... According to Maxine Most , Principal at ...
(Date:12/11/2014)... Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the ... 2015-2019" report to their offering. ... is the adoption of multimodal biometric systems. Multimodal ... an individual for verification and identification purposes. This ...
(Date:12/10/2014)... 8, 2014 You,ve been here before: you desperately ... remember your password, site key or the answer to your ... grade teacher? Today, Hoyos Labs , ... that will finally put an end to the frustration that ... . 1U leverages a user,s smartphone to acquire his ...
Breaking Biology News(10 mins):Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3
... A group of signaling proteins known as Wnt - ... and other tissues - depend on a complex delivery ... cell sites, according to a study at Cincinnati Childrens ... Retromer Complex - breaks down, the delivery of this ...
... Researchers at the Case Western Reserve University School of ... potentially impacting the three billion people exposed to malaria ... Ph. D., and Christopher L. King, M.D., Ph.D. published ... Medicine (PLoS Medicine), novel findings proving new antibodies inhibit ...
... as likely as U.S-born children to suffer from lead poisoning ... study, and the risk is highest among the most recent ... in 2002, published online in the American Journal of Public ... within the previous six months were 11 times as likely ...
Cached Biology News:Certain diseases, birth defects may be linked to failure of protein recycling system 2CWRU School of Medicine has evidence vaccine against malaria will reduce disease 2Study shows immigrant children are at increased risk of lead poisoning 2Study shows immigrant children are at increased risk of lead poisoning 3
(Date:12/24/2014)... 23, 2014 Vermillion, Inc. (Nasdaq:   ... disease, announced today that investors including Oracle Investment ... LLC and several Vermillion directors have agreed to ... Vermillion,s common stock and warrants to purchase unregistered ... placement.  Under the terms of ...
(Date:12/24/2014)... , Dec. 23, 2014  Rock Creek Pharmaceuticals, ... it has filed a Clinical Trial Application (CTA) with ... products Regulatory Agency (MHRA) seeking regulatory approval to initiate ... molecule, Anatabine Citrate. Contingent on the Company ... a Phase I trial to assess the safety, tolerability ...
(Date:12/22/2014)... 22, 2014   Synthetic Biologics, Inc. ... pathogen-specific therapies for serious infections and diseases, ... today announced positive topline safety and tolerability ... of SYN-004, the Company,s investigational oral beta-lactamase ... difficile (C. difficile) infection, antibiotic-associated diarrhea ...
(Date:12/22/2014)... 2014 GenomeDx Biosciences today announced that a ... a genomic test for prostate cancer, was able to ... by radical prostatectomy without adjuvant therapy. Although rapid metastasis ... using tumor genomics to identify these men who have ... important advance. The study has been published online ...
Breaking Biology Technology:Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 2Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 4Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 5Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 2Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 3
... Mass., Dec. 20 Thermo Fisher,Scientific Inc. ... in serving science, today,announced it has acquired ... consumables and related accessories, based in Langerwehe,Germany. ... will allow,Thermo Fisher to offer customers an ...
... CRGN ), a clinical-stage biopharmaceutical company focused ... as Vice,President and Chief Financial Officer effective January ... Wurzer will step down from his position,effective January ... remain,with the Company through March 2008, as an ...
... Inc. (Nasdaq: HGSI ) today announced that ... licensing and collaboration agreement with,Aegera Therapeutics, Inc. as ... 2 trial of HGS-ETR1 in combination with chemotherapy., ... conference call will be hosted by senior management ...
Cached Biology Technology:Thermo Fisher Scientific Acquires Leading Chromatography Consumables Provider, La-Pha-Pack 2Thermo Fisher Scientific Acquires Leading Chromatography Consumables Provider, La-Pha-Pack 3Thermo Fisher Scientific Acquires Leading Chromatography Consumables Provider, La-Pha-Pack 4CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 2CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 3CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 4Human Genome Sciences to Sponsor Conference Call 2
... strains are K-12 derivatives that have mutations ... glutathione reductase (gor) genes, which greatly enhances ... have shown that expression in Origami(DE3) yielded ... host even though overall expression levels were ...
Mouse Aminopeptidase P2/XPNPEP2 Affinity Purified PAb Protein Family: Aminopeptidases, Metalloproteases...
Donor Horse Serum US Origin...
Request Info...
Biology Products: