Navigation Links
Random gene expression may drive HIV into hiding

ocess could be at work in HIV. No one had ever shown that this type of "noise" in gene expression could influence phenotype in infected mammalian cells, Weinberger said.

"I thought it was a cool idea," he added, but "at the time, there wasn't a lot of data to support it. It was pretty far out there."

Weinberger, Arkin, and their colleagues created a model HIV-1 vector--a virus that could enter human cells, carrying with it a key component of HIV's replication machinery: a gene called Tat. Tat facilitates transcription of HIV's entire genome, including itself, which creates a positive-feedback loop: If a little bit of Tat is around, then the HIV genome is transcribed efficiently, which makes more Tat, and so on. If a cell has no Tat, then the HIV genome may remain untranscribed, and, unable to replicate, so the virus heads for latency.

When the scientists infected cultured human cells with their viral vector, they found that cells that initially expressed a low level of virus were very unstable: After a few days, all cells expressed either a high level of virus or none at all.

When Weinberger took one infected human cell with low levels of the virus and allowed it to proliferate into many genetically identical copies of itself, he found that these progeny did not all show the same behavior: some turned their viral expression on high and others turned it off.

This dichotomy in cell fate from genetically identical cells is consistent with the idea that random fluctuations in gene expression control what happens to the cells, the researchers said. To see if this really was the best explanation, they conducted a large array of experimental controls to discount other hypotheses and then created computer programs to model what would happen to the virus under different cellular conditions.

Of the 16 models they tested, only one produced results that matched those seen experimentally in the infected cells. In this model, after
'"/>

Source:Howard Hughes Medical Institute


Page: 1 2 3 4

Related biology news :

1. Alcohols effects on gene expression in the central nervous system
2. Carnegie Mellon scientists develop tool that uses MRI to visualize gene expression in living animals
3. Towards precise classification of cancers based on robust gene functional expression profiles
4. Expression Project for Oncology (expO) completes first phase of standardized gene expression analyses
5. Study: homemade gene expression technology unreliable
6. Cooperation is key—a new way of looking at MicroRNA and how it controls gene expression
7. Agilent Technologies releases probe sequence, annotation information for all its commercial gene expression microarrays
8. Multiple-drug resistant gene expression pattern predicts treatment outcome for pediatric leukemia
9. Computational verification of protein-protein interactions by orthologous co-expression
10. Confirmation of human protein interaction data by human expression data
11. Divergent life history shapes gene expression in brains of salmon
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Random gene expression may drive HIV into hiding

(Date:7/30/2014)... treatment has been shown to relieve patients, pain. ... transmission between the peripheral and central nervous systems, ... Radiation Medicine, Chinese Academy of Medical Sciences, China ... dorsal root ganglia (DRG) to provide relief for ... 14.8, 29.6 MBq) were implanted separately into the ...
(Date:7/30/2014)... of cells types. Researchers would like to understand how to ... that make up the organs and other structures of living ... of sugars that dangle from proteins on surfaces of cells. ... Diego, has created synthetic molecules that can stand in for ... direct the process, they report in the Journal of ...
(Date:7/30/2014)... Calif. , July 30, 2014 ... with the United States Air Force Research Laboratory ... by Binghamton University to ... performance monitoring (HPM). The $425,000 project, with contributions ... packaging firm i3 Electronics, Inc. ( Endicott, ...
Breaking Biology News(10 mins):Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 2Nano-Bio Manufacturing Consortium Awards Funding to Binghamton University for Development of Electronics and Biometric Sensor Platforms 3
... Researchers studying a type of cell found in the trillions ... how it responds to brain injury and disease such as ... trigger the processes that underlie how astrocyte cells respond to ... humans, are a type of glial cell that comprise one ...
... 25, 2013 , , , WHEN:               ... 1, 2013 , , , , LOCATION:       ... , , , SPEAKER:        , , , ... Augmented reality (AR) is here to ...
... of regular aerial surveys and genetics data collected by a ... the central Gulf of Maine as a mating ground for ... online in the journal Endangered Species Research . ... one of the most endangered marine mammal species in the ...
Cached Biology News:Key target responsible for triggering detrimental effects in brain trauma identified 2The (Augmented) Reality of the Situation: Emerging Trends and Issues 2The (Augmented) Reality of the Situation: Emerging Trends and Issues 3Are North Atlantic right whales mating in the Gulf of Maine? 2Are North Atlantic right whales mating in the Gulf of Maine? 3
(Date:7/30/2014)... 2014 Researchers often use microelectronic devices ... such devices can do much more – perhaps ... researchers have looked to patterned assemblies of proteins ... engineering to characterize – and potentially control – ... envisioned to recreate animal and human physiological functions ...
(Date:7/30/2014)... IRVINE, Calif. , July 30, 2014 /PRNewswire/ ... on the research and development of compounds to ... in humans, today announces that its partner, CardioNova, ... Phase 1b clinical trial with AtheroNova,s lead compound, ... continuation of the AHRO-001 Phase 1 safety trial ...
(Date:7/30/2014)... 2014 NCERC at Southern Illinois ... his findings on corn stover pretreatment methods at ... in Washington, D.C. , “Arun’s selection for ... a testament to the success of our postdoctoral ... to the foresight and vision of SIUE and ...
(Date:7/30/2014)... CA (PRWEB) July 30, 2014 ... from the U.S. Environmental Protection Agency (EPA). This ... in developmental toxicity models, including tests designed to ... formation and the formation of connections between neurons ... of neurodifferentiation and synapse formation by environmental pollutants ...
Breaking Biology Technology:UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 2AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 3AtheroNova Announces Milestone with Initiation of Phase 1b Clinical Trial of AHRO-001 4NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 2NCERC at SIUE Researcher Takes Stage at Department of Energy Conference 3U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 2U.S. Environmental Protection Agency (EPA) Awards Vala Sciences a Second ToxCast Contract 3
... PARK, N.C. and MIAMI, Oct. 13 The Alpha-1 ... grants to study how genetic variations in the alpha-1 ... in people with AAT deficiency. The inherited condition causes ... whether AAT infusion therapy -- known as augmentation therapy ...
... , CHICAGO, Oct. 13 Advanced Life Sciences ... company engaged in the discovery, development and commercialization ... infection, cancer and respiratory diseases, today announced that ... held October 28-29th in San Francisco. , (Logo: ...
... LONDON, October 13 Systagenix Wound Management ... Board, consisting of,a group of multi-disciplinary and highly respected ... which will be led by,Systagenix,s newly appointed Medical Director, ... major specialist organisations and thought leaders in the,wound care ...
Cached Biology Technology:The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 2The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 3The Alpha-1 Foundation Awards Two Research Grants with Support from Talecris Biotherapeutics, Inc. 4Advanced Life Sciences to Present at 8th Annual BIO Investor Forum 2Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 2Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 3Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 4Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 5Systagenix Announces Formation of New Global Medical Advisory Board to Support Future Clinical Advances in Wound Care 6