Navigation Links
Quantum Dots Research Leads to New Knowledge about Protein Binding in Plants

UC Riverside researchers from the Departments of Chemical and Environmental Engineering, Mechanical Engineering and Botany and Plant Sciences have worked together to discover a way to utilize Quantum Dot bio-conjugates to uncover new knowledge about the binding of a protein at the growing pollen tube tip. This protein plays a critical role along with another protein (chemocyanin) in guiding sperm-laden pollen tubes to the eggs found in ovules.

Applying nanoparticles for imaging the protein localization revealed information that could not be observed previously by conventional imaging techniques. This study provides a new tool to botanical scientists by merging areas of materials science, chemistry and plant biology. The findings are the result of an interdisciplinary research team including Sathyajith Ravindran of the Chemical and Environmental Engineering Department; Sunran Kim, Rebecca Martin and Elizabeth M. Lord of the Botany and Plant Sciences Department; and Cengiz S. Ozkan of the Mechanical Engineering Department at UC-Riverside. The results of their collaborative research appeared in an article titled “Quantum Dots as Bio-labels for the Localization of a Small Plant Adhesion Protein?and published in the January 2005 issue of Nanotechnology, and is a featured article at Journal Nanotechnology has an international readership among academic, government and corporate sectors, and is dedicated to coverage of all aspects of nanoscale science and technology from a multidisciplinary perspective. Ozkan and his colleagues utilized cadmium selenide (CdSe) quantum dots coated with zinc sulphide as fluorescent probes. The particles had a diameter of 6.3 nm. The team terminated the quantum dots with carboxylic groups by reacting them with mercaptoacetic acid. Then they conjugated the quantum dots with the amine groups of stigma/stylar cysteine-rich adhesin (SCA) - a plant polle n tube adhesion protein. This labeled the protein molecules with fluorescent tags. Quantum dots are much more resistant to photobleaching than conventional fluorescent markers and their small size make them ideal for biological imaging. The researchers then added the molecules to germinated lily pollen grains and examined them under a confocal microscope. This is the very first time that Quantum Dots have been utilized for live imaging in plant systems. The study opens the door for the potential use of Quantum Dots in live imaging of plant cells and provides valuable understanding of the mechanism of interaction between the pollen tube and female tissue during reproduction. “Integrating materials science, chemistry and plant biology to understand how and where specific proteins act on a pollen tube is one more step towards a better understanding of the fundamental processes involved, namely the network of the signaling process in plant reproduction,?said Ozkan. "A better understanding of the interaction of SCA with pollen tubes could help with successful plant breeding?

Source:UCR News

Related : QuantumDot

Related biology news :

1. Quantum dots provide a faster, more sensitive method for detecting respiratory viral infections
2. Connect the Quantum Dots
3. Quantum dots reviewed -- Could these nanoparticles hold the cure to cancer?
4. First Quantum Grant to fund stem cell repair of damage from stroke
5. Researchers discover way to make cells in the eye sensitive to light
6. Researchers find how protein allows insects to detect and respond to pheromones
7. Researchers Uncover Key Step In Manufacture of Memory Protein
8. Research advances quest for HIV-1 vaccine
9. Research on Worms Yields Clues on Aging
10. Researchers reveal the infectious impact of salmon farms on wild salmon
11. Researchers identify target for cancer drugs

Post Your Comments:

(Date:11/30/2015)... DEERFIELD BEACH, Fla. , Nov. 30, 2015 ... selected as a finalist in this year,s Fierce Innovation ... publisher of FierceHealthIT , ... BIOCLAIM was recognized as a finalist in the ... --> ...
(Date:11/18/2015)... -- As new scientific discoveries deepen our understanding of how ... face challenges in better using that knowledge to guide ... more children continue to survive pediatric cancer, that counseling ... John M. Maris, M.D ., a pediatric ... . --> John M. Maris, M.D ...
(Date:11/17/2015)... Mass. , Nov. 17, 2015 Pressure ... leader in the development and sale of broadly enabling, ... worldwide life sciences industry, today announced it has received ... its $5 million Private Placement (the "Offering"), increasing the ... $4,025,000.  One or more additional closings are expected in ...
Breaking Biology News(10 mins):
(Date:12/1/2015)... DUBLIN , December 1, 2015 /PRNewswire/ ... the addition of the  "2016 U.K. Virology ... Segment Forecasts for 100 Tests, Supplier Shares ... Opportunities"  report to their offering.  --> ... of the  "2016 U.K. Virology and Bacteriology ...
(Date:11/30/2015)... ... November 30, 2015 , ... Global Stem Cells Group announced that ... closed system for isolating adipose-derived stem cells. The announcement starts a new phase toward ... SVF is a component of the lipoaspirate obtained from liposuction of excess adipose tissue. ...
(Date:11/30/2015)... ... ... Cells Group announced the opening of a new core patient care hub with the ... The facilities are part of GSCG’s expansion efforts in Latin America. , Both the ... to patients from around the world. , The clinics will be headed by Victor Perez, ...
(Date:11/30/2015)... Dec. 1, 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ), ... joined the American Business Act on Climate Pledge, alongside more ... standing with the Obama Administration to demonstrate an ongoing commitment ... outcome to the COP21 Paris climate ... Sarnia, Canada . --> BioAmber ...
Breaking Biology Technology: