( in jet engines. We adapted some id...)Purdue creates new low-cost system to detect bacteria

"We adapted some ideas from that research to build a scatterometer for food safety, and now we're using the second generation of that instrument," Hirleman said.

A major motivation for the research is to reduce the time it takes for industry to identify harmful organisms in food processing. Scientists in food-processing plants routinely grow cultures to test for dangerous pathogens.

"The dairy industry, for example, grows bacteria on petri dishes to make sure products are safe, but industry is trying to develop technologies that will very quickly identify organisms," Robinson said. "The same sort of thing holds true for clinical microbiology and other laboratories. With our light-scattering method, it takes less than five minutes to identify harmful organisms after they have grown in a petri dish. The analysis is faster than any other methods in existence, and it's simple."

The technique might be used to identify staph infections that are resistant to antibiotics.

"This is an extremely dangerous infection, and you want to catch it as early as possible," Robinson said.

A mass-produced system based on the technology would consist of inexpensive, off-the-shelf hardware, such as red lasers and low-resolution digital cameras available at consumer electronics stores, and likely would cost less than $1,000, Hirleman said.

A critical part of the technique was made possible by adapting a mathematical method created in 1934 by Dutch physicist Fritz Zernike, who created a set of mathematical "descriptors" subsequently called radial Zernike polynomials. These descriptors can be used to analyze how light-wave patterns are distorted after passing through lenses having complex flaws or aberrations.

Individual bacterial colonies growing in a petri dish also distort light passing through them, just as a lens changes light-wave patterns.

"Therefore, we can treat the colonies as lenses and
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Source:Purdue University