Navigation Links
Pulsating ultrasound enhances gene therapy for tumors

High-intensity focused ultrasound emitted in short pulses is a promising, non-invasive procedure for enhancing gene delivery to cancerous cells without destroying healthy tissue, according to a study in the May issue of the journal Radiology.

High-intensity focused ultrasound (HIFU) is more powerful than standard ultrasound. HIFU can destroy tumors through long and continuous exposures that raise the temperature inside cancerous cells, effectively "cooking" them. Under a technique introduced by King C.P. Li, M.D., M.B.A., from the National Institutes of Health (NIH), short pulses of HIFU can be used to prevent exposed tissue from becoming too hot and damaged. Pulsed-HIFU instead renders tissues permeable and helps target them for taking up genes and other therapeutic substances injected into the body.

"Basically, we're using sound waves to open up the tissue by producing gaps between the cells, making it leakier and more prone to taking up various genes, agents and compounds," said Victor Frenkel, Ph.D., a staff scientist for the diagnostic radiology department at the NIH Clinical Center in Bethesda, Md.

Working with lead authors Kristin M. Dittmar, M.D., and Jianwu Xie, M.D., the researchers used pulsed-HIFU on tumors in mice, then immediately injected an easily measurable reporter gene into the vein in their tails. The reporter gene in this study--a fluorescent-green protein found in deep-sea invertebrates--was visible in all sections of the tumors exposed to pulsed-HIFU. Tumors not targeted with pulsed-HIFU showed negligible signs of the gene.

An analysis showed reporter gene levels to be nine times higher in tumors treated with pulsed-HIFU compared with tumors left unexposed.

Researchers were especially encouraged by the results because the type of cancer treated in the study--squamous cell carcinoma, found in head and neck tumors--is one of the least permeable cancers and does not respond well to chemotherapy or radiation. However, these types of tumors have responded to certain types of therapeutic genes.

"This procedure is hypothetically generic for enhancing delivery to all tissues," Dr. Frenkel said. "Previous studies by Dr. Li have shown that pulsed-HIFU increases the uptake of drugs. Now we've shown that it works for genes and we're making the case that there's a connection between the two."

Other methods currently being investigated for enhancing gene delivery, such as lasers and electric current, are limited to surface lesions or require needles to be inserted in the body. Pulsed-HIFU is non-invasive and can treat any area of the body accessible by ultrasound, the exceptions being the lungs and bones. Additional advantages of pulsed-HIFU include no scarring, limited blood loss and infections, reduced risk of other complications, shortened recovery time, significant reduction in costs and the potential for many procedures to be done on an outpatient basis.


'"/>

Source:Radiological Society of North America


Related biology news :

1. Penn researchers study the use of ultrasound for treatment of cancer
2. 3D ultrasound device poised to advance minimally invasive surgery
3. 3-D ultrasound scanner could guide robotic surgeries
4. 3-D ultrasound identifies women at risk for impending preterm birth
5. Elevated temperature enhances success of viral cancer therapy
6. NYU algorithm enhances ability to detect cancer genes
7. Proteasome activator enhances survival of Huntingtons disease neuronal model cells
8. Adding Radiation Therapy To Chemotherapy Improves Survival In Patients With High-risk Breast Cancer
9. Columbia research lifts major hurdle to gene therapy for cancer
10. Combination therapy boosts effectiveness of telomere-directed cancer cell death
11. Gene therapy converts dead bone graft to new, living tissue
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/20/2016)... , May 20, 2016  VoiceIt is ... partnership with VoicePass. By working together, ... experience.  Because VoiceIt and VoicePass take slightly different ... engines increases both security and usability. ... excitement about this new partnership. "This ...
(Date:4/28/2016)... BANGALORE, India , April 28, 2016 ... subsidiary of Infosys (NYSE: INFY ), and Samsung ... global partnership that will provide end customers with a ... and payment services.      (Logo: http://photos.prnewswire.com/prnh/20130122/589162 ... for financial services, but it also plays a fundamental part ...
(Date:4/15/2016)... CHICAGO , April 15, 2016  A ... companies make more accurate underwriting decisions in a ... offering timely, competitively priced and high-value life insurance ... health screenings. With Force Diagnostics, rapid ... and lifestyle data readings (blood pressure, weight, pulse, ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... ... ... While the majority of commercial spectrophotometers and fluorometers use the z-dimension of 8.5 ... end machines that use the more unconventional z-dimension of 20mm. Z-dimension or ... the cuvette holder. , FireflySci has developed several Agilent flow cell product lines ...
(Date:6/23/2016)... ... 23, 2016 , ... UAS LifeSciences, one of the leading ... UP4™ Probiotics, into Target stores nationwide. The company, which has been manufacturing high ... its list of well-respected retailers. This list includes such fine stores as Whole ...
(Date:6/23/2016)... SILVER SPRING, Md. , June 23, 2016 ... evidence collected from the crime scene to track the criminal ... sick, and the U.S. Food and Drug Administration (FDA) uses ... Sound far-fetched? It,s not. ... whole genome sequencing to support investigations of foodborne illnesses. Put ...
(Date:6/23/2016)...  The Prostate Cancer Foundation (PCF) is pleased to announce 24 ... for prostate cancer. Members of the Class of 2016 were selected from a ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
Breaking Biology Technology: