Navigation Links
Pulsating ultrasound enhances gene therapy for tumors

High-intensity focused ultrasound emitted in short pulses is a promising, non-invasive procedure for enhancing gene delivery to cancerous cells without destroying healthy tissue, according to a study in the May issue of the journal Radiology.

High-intensity focused ultrasound (HIFU) is more powerful than standard ultrasound. HIFU can destroy tumors through long and continuous exposures that raise the temperature inside cancerous cells, effectively "cooking" them. Under a technique introduced by King C.P. Li, M.D., M.B.A., from the National Institutes of Health (NIH), short pulses of HIFU can be used to prevent exposed tissue from becoming too hot and damaged. Pulsed-HIFU instead renders tissues permeable and helps target them for taking up genes and other therapeutic substances injected into the body.

"Basically, we're using sound waves to open up the tissue by producing gaps between the cells, making it leakier and more prone to taking up various genes, agents and compounds," said Victor Frenkel, Ph.D., a staff scientist for the diagnostic radiology department at the NIH Clinical Center in Bethesda, Md.

Working with lead authors Kristin M. Dittmar, M.D., and Jianwu Xie, M.D., the researchers used pulsed-HIFU on tumors in mice, then immediately injected an easily measurable reporter gene into the vein in their tails. The reporter gene in this study--a fluorescent-green protein found in deep-sea invertebrates--was visible in all sections of the tumors exposed to pulsed-HIFU. Tumors not targeted with pulsed-HIFU showed negligible signs of the gene.

An analysis showed reporter gene levels to be nine times higher in tumors treated with pulsed-HIFU compared with tumors left unexposed.

Researchers were especially encouraged by the results because the type of cancer treated in the study--squamous cell carcinoma, found in head and neck tumors--is one of the least permeable cancers and does not respond well to chemotherapy or radiation. However, these types of tumors have responded to certain types of therapeutic genes.

"This procedure is hypothetically generic for enhancing delivery to all tissues," Dr. Frenkel said. "Previous studies by Dr. Li have shown that pulsed-HIFU increases the uptake of drugs. Now we've shown that it works for genes and we're making the case that there's a connection between the two."

Other methods currently being investigated for enhancing gene delivery, such as lasers and electric current, are limited to surface lesions or require needles to be inserted in the body. Pulsed-HIFU is non-invasive and can treat any area of the body accessible by ultrasound, the exceptions being the lungs and bones. Additional advantages of pulsed-HIFU include no scarring, limited blood loss and infections, reduced risk of other complications, shortened recovery time, significant reduction in costs and the potential for many procedures to be done on an outpatient basis.


'"/>

Source:Radiological Society of North America


Related biology news :

1. Penn researchers study the use of ultrasound for treatment of cancer
2. 3D ultrasound device poised to advance minimally invasive surgery
3. 3-D ultrasound scanner could guide robotic surgeries
4. 3-D ultrasound identifies women at risk for impending preterm birth
5. Elevated temperature enhances success of viral cancer therapy
6. NYU algorithm enhances ability to detect cancer genes
7. Proteasome activator enhances survival of Huntingtons disease neuronal model cells
8. Adding Radiation Therapy To Chemotherapy Improves Survival In Patients With High-risk Breast Cancer
9. Columbia research lifts major hurdle to gene therapy for cancer
10. Combination therapy boosts effectiveness of telomere-directed cancer cell death
11. Gene therapy converts dead bone graft to new, living tissue
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/21/2017)... 2017 Der weltweite Biobanking-Sektor wird ... einem Gespräch mit mehr als 50 Vertretern aus verschiedenen Branchen ... gilt, um diese Prognose zu realisieren. ... Zu den ... finanziellen Mittel für die Biobank, die Implementierung Zeit sparender ...
(Date:2/14/2017)... N.C. , Feb. 14, 2017  Wake Forest ... M.D., as its new chief executive officer (CEO). Freischlag ... CEO John D. McConnell , M.D., who last ... position at the Medical Center, after leading it since ... the full scope of Wake Forest Baptist,s academic health ...
(Date:2/13/2017)... WASHINGTON , Feb. 13, 2017 Former ... U.S. Senate Judiciary Committee, Janice Kephart of ... regarding President Donald Trump,s "Executive Order: Protecting ... States" (Jan. 27, 2017):  "As President Trump,s ... 9th Circuit has now essentially banned the travel ban, ...
Breaking Biology News(10 mins):
(Date:3/27/2017)... ... March 27, 2017 , ... IsoPlexis Corporation (IsoPlexis), a ... disease and more through a single-cell precision engineering platform, today announced it has ... in the laboratory of Dr. James Heath at the California Institute of ...
(Date:3/27/2017)... ... March 27, 2017 , ... Biopsies from non-small cell ... with limited tumor content in a large background of normal or wild type ... need for reliable detection of low abundance somatic mutations, particularly in small specimens ...
(Date:3/27/2017)... Md. , March 27, 2017  The ... billion for 2016, according to a new report ... medical lab testing is performed to evaluate disease ... individual therapy, among other reasons.  The healthcare market ... Market , provides an overview of the ...
(Date:3/27/2017)... The new research portal will give visitors quick ... Valero Energy , offering extensive market research on their ... ... The latest trend gaining momentum in ... even though touted as a green alternative to fossil fuels, ...
Breaking Biology Technology: