Navigation Links
Primitive yeast yields secrets of human cholesterol and drug metabolism

By first probing the way primitive yeast make cholesterol, a team of scientists has discovered a long-sought protein whose human counterpart controls cholesterol production and potentially drug metabolism.

The collaborative study by investigators at Johns Hopkins University School of Medicine, Vanderbilt University, Indiana University and Eli Lilly Co., was published in the February issue of Cell Metabolism.

“Dap1 controls the activity of a clinically important class of enzymes required for cholesterol synthesis and drug metabolism,?says Peter Espenshade, Ph.D., assistant professor of cell biology at Johns Hopkins. “We’re excited because although we originally identified this protein in yeast, humans not only have the same protein, but it works the same way.?

The search for Dap1 began with the hunt for factors that influence the actions of a large family of enzymes called cytochrome P450. These enzymes control many life-sustaining chemical reactions in humans and other animals.

Happily, Espenshade says, yeast have only two P450 enzymes, and both play roles in making cholesterol, narrowing down the territory for their search and giving them a telltale marker (the cholesterol) to track.

Reasoning that whatever controls the P450s likely would be turned on and off at roughly the same time as the P450 enzymes themselves, the researchers found that Dap1 does just that in the yeast cell.

To figure out what Dap1 does, Espenshade and colleagues genetically altered yeast cells to lack Dap1. Those cells predictably were unable to make cholesterol and instead contained a build-up of cholesterol precursors.

The research team then tracked Dap1’s counterpart in humans by looking for similar proteins in a computer database and repeated their experiments in human kidney cells engineered to lack the human version of Dap1. As in yeast, the altered human cells accumulated cholesterol precursors and died because cholest
'"/>

Source:Johns Hopkins Medical Institutions


Page: 1 2

Related biology news :

1. Examination of internal wiring of yeast, worm, and fly reveals conserved circuits
2. Navigating an integrated yeast network
3. After the yeast is gone bacteria continue to develop flavor of sparkling wine
4. GlycoFi announces the first production of antibodies with human glycosylation in yeast
5. From a lowly yeast, researchers divine a clue to human disease
6. GlycoFi and Dartmouth report full humanization of yeast glycosylation pathway in Science
7. Engineered yeast speeds ethanol production
8. Hives ferment a yeasty brew, attract beetle pest
9. Genome-wide mouse study yields link to human leukemia
10. Marine sponge yields nanoscale secrets
11. Small worm yields big clue on muscle receptor action
Post Your Comments:
(Date:3/24/2015)... and Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) has announced the addition ... Report" report to their offering. This report analyzes ... by the following Segments: Face Biometrics, and Voice Biometrics. The report ... , Japan , Europe , ... & Africa , and Latin America ...
(Date:3/23/2015)... BOSTON, Mass , March 23, 2015 SoundView ... shipment of NXT-ID,s (NASDAQ: NXTD ) Wocket smart wallet. SoundView ... world user feedback on their experience with the Wocket in ... experience at CVS, Whole Foods and other retailers, making both ... Kris Tuttle also says, "If the company meets ...
(Date:3/23/2015)... Mar. 23, 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... the growing mobile commerce market, announces its biometric payment technology, ... on CNBC television starting March 30 th . ... in New York markets. ... are excited about our new ad campaign following the recent ...
Breaking Biology News(10 mins):Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3
... 19, 2013  Organovo Holdings, Inc. (NYSE MKT: ONVO) (" Organovo ... bioprinting technology, has been invited to present at the 25th ... New York December 3-4, 2013. ... Murphy , will be presenting on Wednesday, December 4, at ...
... great white shark, a major apex predator made famous by ... species capturing an extraordinary amount of public fascination. An intriguing ... way to address this is to explore the genetic makeup ... scientists from Nova Southeastern University,s (NSU) Save Our Seas Shark ...
... of annual change in sea-ice cover can been seen in ... new study from the University of Toronto Mississauga (UTM). ... used to track changes in Arctic sea ice," says Jochen ... Physical Sciences. "We found the algal record shows a dramatic ...
Cached Biology News:Organovo to Present at the 25th Annual Piper Jaffray Healthcare Conference 2Genome scale view of great white shark uncovers unexpected and distinctive features 2Genome scale view of great white shark uncovers unexpected and distinctive features 3Underwater 'tree rings' 2
(Date:5/1/2015)... 1, 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ... that it has priced an underwritten registered offering of ... of $9.00 per share, and granted the underwriters in ... an additional 585,000 shares of its common stock. The ... offering is expected to close on May 6, 2015, ...
(Date:5/1/2015)... 1, 2015 Investor-Edge has ... Ltd (NASDAQ: GENE ), Agilent Technologies Inc. ... (NYSE: TMO ), Sequenom Inc. (NASDAQ: ... ). Free research report on Genetic Technologies can ... April 30, 2015, the NASDAQ Composite ended at ...
(Date:4/30/2015)... 01, 2015 Spirax Sarco, the leader ... proud to announce that for the first time in ... winner of a Queen’s Award for Innovation – the ... and manufacture of a unique flowmeter. , The Spirax ... to traditional steam flow measurement. The device provides ...
(Date:4/30/2015)... , April 30, 2015 /PRNewswire/ - BioAmber Inc. ... sustainable chemicals, today announced that it has commenced an underwritten ... expects to grant the underwriters a 30-day option to ... shares of common stock offered in the public offering. ... can be no assurance as to whether or when ...
Breaking Biology Technology:BioAmber Inc. Announces Pricing Of $35.1 Million Registered Offering 2BioAmber Inc. Announces Pricing Of $35.1 Million Registered Offering 3Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 2Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 3Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 4Life Sciences Tools and Services Stocks on our Radar -- Genetic Technologies, Agilent Technologies, Thermo Fisher Scientific, Sequenom, and PerkinElmer 5Spirax Sarco Receives a Queen’s Award for Innovation 2Spirax Sarco Receives a Queen’s Award for Innovation 3BioAmber Inc. Announces Public Offering Of Common Stock 2BioAmber Inc. Announces Public Offering Of Common Stock 3
... Multiporator , , , , , , , ... Protocol No. 4308 915.050 11/2001 , , , ... , Cell line , ... Transfection with , pcDNA3.1/V5-His-TOPO (in bidistilled H 2 O), ...
... , Multiporator / Electroporator 2510 , , , , , , ... Transformation Protocol , Protocol No. 4308 915.536 01/2002 , ... , , , , , ... , , Cell type ...
... , , , , , Multiporator / Electroporator 2510 , , , ... Transformation Protocol , Protocol No. 4308 915.533 01/2002 , ... , , , , , ... , Cell type , Bacteria, ...
Cached Biology Technology:721.221 2Ustilago maydis 2Ustilago maydis 3Staphylococcus aureus 2
...
Mouse monoclonal antibody raised against a partial recombinant WDR5. NCBI Entrez Gene ID = WDR5...
... Polyclonal Antibody against Nonmuscle Myosin ... Purified Antibody (Stored in TBS + ... against the peptide SDVNETQPPQSE corresponding to ... chain isoform B (MHC-B) This antibody ...
... Phosphate Phosphatase 2 (LPP2) ... aqueous solution Solution ... containing 0.08% sodium azide. ... the human LPP2 protein. ...
Biology Products: