Navigation Links
Precision biochemistry tracks DNA damage in fish

Like coal-mine canaries, fish DNA can serve as a measure of the biological impact of water and sediment pollution--or pollution clean-up. That's one of the conclusions of a new study* by researchers from the Pacific Northwest Research Institute (PNRI), Woods Hole Oceanographic Institution, the University of Maryland and the National Institute of Standards and Technology (NIST).

Research over the past several years has demonstrated the adverse effects of industrial pollutants in water and sediment on the health of fish in the lower Duwamish River. The Duwamish flows through an industrialized section of south Seattle, Wash., and in 2001 a section of the lower river was added to the Environmental Protection Agency's Superfund list because of contaminants including polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), mercury and other metals, and phthalates. In previous research on Duwamish fish, PNRI used an infrared spectroscopy method to document DNA damage in the gills of English sole.

In a new joint paper, the researchers report on several biomarkers, including pollution-induced P450 enzyme changes, and on infrared spectral analysis of DNA and measurements of specific modifications to DNA from fish gills and livers using liquid and gas chromatography combined with mass spectrometry (LC/MS,GC/MS). Precision chemical analysis techniques at NIST allowed the researchers to identify and measure damage to adenine and guanine, specific chemical components or bases of DNA, at extraordinarily low levels--five lesions out of a 100 million bases in one case. The results correlated well with earlier research and revealed similar damage to liver DNA (more likely tied to the fish's food) and gill DNA (more probably reflecting pollutants in water).

The results suggest that these DNA lesions, and others like them, can be used as very sensitive biomarkers to provide a direct measure of the impact of contaminants on fish populations. More over, since natural DNA repair processes may gradually reverse the damage in the absence of further insults, these biomarkers also might be used to help assess the efficacy of pollution remediation efforts. The work was funded in part by the National Institute of Environmental Health Sciences (NIH).


'"/>

Source:National Institute of Standards and Technology (NIST)


Related biology news :

1. Protein offers way to stop microscopic parasites in their tracks
2. Stopping smallpox in its tracks: A new anti-viral approach
3. Venom doc tracks down snake bioweapons
4. MERIS monitoring tracks planetary photosynthesis levels
5. U. of Colorado researcher identifies tracks of swimming dinosaur in Wyoming
6. Magnetic probe successfully tracks implanted cells in cancer patients
7. Research tracks whales by listening to sounds
8. New tool tracks brain development in babies
9. Ghost protein leaves fresh tracks in the cell
10. New study aims to stop sepsis in its tracks
11. New imaging technique tracks traffic patterns of white blood cells
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/3/2016)... , Feb. 3, 2016 ... addition of the "Emotion Detection and ... Learning, and Others), Software Tools (Facial Expression, ... End Users,and Regions - Global forecast to ... --> http://www.researchandmarkets.com/research/d8zjcd/emotion_detection ) has announced ...
(Date:2/2/2016)... , Feb. 2, 2016 Technology Enhancements Accelerate Growth ... analysis of the digital and computed radiography markets in ... , and Indonesia (TIM). It ... market size, as well as regional market drivers and ... discusses market penetration and market attractiveness, both for digital ...
(Date:2/1/2016)... 1, 2016  Today, the first day of American ... to develop a first of its kind workplace health ... Watson. In the first application of Watson ... IBM ), and Welltok will create a new offering ... cognitive analytics, delivered on Welltok,s health optimization platform. The ...
Breaking Biology News(10 mins):
(Date:2/10/2016)... Feb. 10, 2016  Matchbook, Inc., a company ... growing biotech companies, announced today the appointment of ... Advisor. Jim brings nearly 25 years of experience ... having spent nearly two decades in executive level ... Procurement at Genzyme and, most recently headed global ...
(Date:2/10/2016)... ... February 10, 2016 , ... HOLLOWAY AMERICA, a leading custom ... Rocky Mountain Chapter 21st Annual Vendor Exhibition on Thursday, February 18, 2016. The ... for its annual event, which will run from 3:00 p.m. - 8:30 p.m. ...
(Date:2/10/2016)... ... February 10, 2016 , ... ... comprehensive training and support program, Sonalink™ remote monitoring. The inaugural launch of this ... Friday, February 5th, connecting Dr. Samuel Peretsman to a HIFU technical expert at ...
(Date:2/10/2016)... ... 10, 2016 , ... Curoverse announced today that the open-source, ... capabilities for managing and processing genomic and health data at petabyte scale. ... and analyzing genomic data,” said Adam Berrey chief executive officer at Curoverse. “Microsoft ...
Breaking Biology Technology: