Navigation Links
Plague proteome reveals proteins linked to infection

Recreating growth conditions in flea carriers and mammal hosts, Pacific Northwest National Laboratory scientists have uncovered 176 proteins and likely proteins in the plague-bacterium Yersinia pestis whose numbers rise and fall according to the disease’s virulence.

The team, led by the Department of Energy laboratory staff scientists Mary Lipton and Kim Hixson, identified the proteins as “unique biomarkers related specifically to growth condition,?according to a study in the latest issue of the Journal of Proteome Research. Biomarkers associated with disease progression show promise as detection tools in public health and biodefense and can guide drug and vaccine designers in their quest to disrupt the microbe’s ability to infect.

Y. pestis is the bacterium that caused the infamous Black Death plagues. Fleas are vectors for the disease and can spread it to rodent and human hosts. This study mimicked environmental conditions of Y. pestis in flea and in mammalian systems.

The proteome is a survey of proteins in a cell. Lipton, Hixson and colleagues at the PNNL-based Environmental Molecular Sciences Laboratory and Lawrence Livermore National Laboratory used proteomic techniques called accurate mass and time tag mass spectrometry and clustering analysis to compare abundance changes in 992 proteins under four different growth conditions, at 26 degrees and 37 degrees Celsius and with and without calcium.

They found 89 candidate proteins with similar abundance changes to 29 known virulence-linked proteins, and an additional 87 disease-condition-associated “hypothetical?proteins. The Institute for Genomic Research defines a hypothetical protein as one identified by a gene-finding algorithm that matches no other known protein sequence or contains no other evidence that it is an actual product of a gene.

The study authors said the same approach is being applied to a search for biomarkers across a wide range of biological syst ems, from other infectious agents such as Salmonella to soil microbes of interest in cleaning up toxic waste.
'"/>

Source:DOE/Pacific Northwest National Laboratory


Related biology news :

1. A wolf in sheeps clothing: Plague bacteria reveal one of their virulence tricks
2. Scientists characterize proteome of human cornea
3. A bacterial genome reveals new targets to combat infectious disease
4. First real-time view of developing neurons reveals surprises, say Stanford researchers
5. Examination of internal wiring of yeast, worm, and fly reveals conserved circuits
6. NYU study reveals how brains immune system fights viral encephalitis
7. Study reveals new technique for fingerprinting environmental samples
8. Study reveals dramatic difference between breast cancers in US and Africa
9. UCSD research reveals mechanism involved with type of fatal epilepsy
10. Study reveals candidate targets for anti-retroviral therapeutics
11. Chimp genome reveals a retroviral invasion

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/7/2016)... According to a new market research report "Emotion Detection and Recognition Market ... Application Area, End User, And Region - Global Forecast to 2021", published by ... 2016 to USD 36.07 Billion by 2021, at a Compound Annual Growth Rate ... ... MarketsandMarkets Logo ...
(Date:12/6/2016)... Valencell , the leading innovator in ... a third consecutive year of triple digit growth for ... with a 360 percent increase in companies who have ... driven by sales of its wrist and ear Benchmark™ ... technology for hearables for fitness and healthcare applications. ...
(Date:11/30/2016)... , Nov. 30, 2016 Not many of us realize that we spend ... recovery so we need to do it well. Inadequate sleep levels have been found ... pressure, stroke, diabetes, and even cancer. Maybe now is the best time ... could help them to manage their sleep quality? Continue ... ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... ... December 08, 2016 , ... The ability ... optogenetics — is key to exciting advances in the study and mapping of ... projected via free-space optics stimulates small, transparent organisms and excites neurons within superficial ...
(Date:12/8/2016)... Oxford Gene Technology ... Palette an anpassbaren SureSeq™ NGS-Panels mit dem Start ... ein schnelles und kostengünstiges Studium der Varianten bei ... Erkennung von Einzel-Nukleotid-Variationen (Single Nucleotide Variation, SNV) und ... Panel und ermöglicht eine individuelle Anpassung durch die ...
(Date:12/8/2016)...  Anaconda BioMed S.L., a pre-clinical stage medical device ... neuro-thrombectomy system for the treatment of Acute Ischemic Stroke ... to join its Scientific Advisory Board (SAB). The SAB ... scientific and clinical experts to Anaconda BioMed S.L., as ... ® to its clinical phase. The SAB is ...
(Date:12/8/2016)... (PRWEB) , ... December 08, ... ... announces the commercial launch of flexible packaging for their exceptionally efficient human ... bag system extends RoosterBio’s portfolio of bioprocess media products engineered to radically ...
Breaking Biology Technology: