Navigation Links
Picking particles faster than one at a time

n about ten minutes.

Adiga and his colleagues tested the new algorithm by using it to pick images from among over 130,000 ribosome particles in 55 micrographs provided by the Wadsworth Center of the New York State Department of Health in Albany. Adiga separately inspected the 55 micrographs by eye and "manually" selected particles, well over 80 percent of which turned out to be the same as those picked by the program. Fewer than 10 percent of the images chosen by the program were false positives.

A coauthor of the paper, William Baxter, independently inspected 14 of the same micrographs, chosen at random. On his first pass, intending to select only particles of the highest quality -- a "gold standard" -- he chose roughly two-thirds of the same particles picked by the software. When the program's additional candidates were inspected more closely, however, many turned out to be true positives of good quality; only about 10 percent of the program's picks were false positives.

Similar results were obtained when the segmentation program was used to pick particles from a smaller and more difficult molecule, a convex or "boat-shaped" enzyme labeled TPP-II, isolated from the fruit-fly. Although an initial comparison between manual selection and automatic selection indicated that 15 percent of the program's nominations were false positives, when the program was run again -- using a template after segmentation to filter out incompatible shapes -- false positives dropped to a mere 7 percent.

Beyond the demonstrated goal of selecting the same particles an expert would select with a low error rate, future refinement of the segmentation algorithm aims higher. By concentrating on the highest quality particles, crystallization in silico may need far fewer than hundreds of thousands of particles.

"Jacqueline Milne of the National Cancer Institute has demonstrated that high-quality structural maps can be achieved with a few hundred parti
'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4 5

Related biology news :

1. Picking apart how neurons learn
2. DNA Molecules Used To Assemble Nanoparticles
3. Tiny particles could solve billion-dollar problem
4. Imaging Lymph Nodes with Nanoparticles
5. Probing The Promise And Perils Of Nanoparticles
6. Probing the promise and perils of nanoparticles
7. Nanoparticles offer new hope for detection and treatment
8. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma
9. Basis for DNA ejection from single phage particles
10. Using nanoparticles, in vivo gene therapy activates brain stem cells
11. Breakthrough: Scientists create worlds tiniest organic particles
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Picking particles faster than one time

(Date:8/27/2014)... traps are lost or abandoned each year in U.S. ... which continue to catch fish, crabs, and other species ... habitat, fisheries, and the watermen who depend on the ... published NOAA study. , The report, published in the ... its kind to examine the derelict fish trap problem, ...
(Date:8/27/2014)... A growing number of studies have shown ... the pathogenesis of Parkinson,s disease. Previous studies ... Medical University in China have shown that ... injury, reduced divalent metal transporter 1 expression, ... substantia nigra of rotenone-induced Parkinson,s disease rats. ...
(Date:8/27/2014)... Adelaide research has shown that piglets can be weaned ... , The outcome of the study at the University,s ... Science , is an important finding for pig producers. ... loss of production. , "Sows don,t usually start their ... after their piglets have been weaned," says Ms Alice ...
Breaking Biology News(10 mins):NOAA's Marine Debris Program reports on the national issue of derelict fishing traps 2Piglet weaning age no bar to litter frequency 2
... & Children,s Hospital and the University of Adelaide, Australia, ... with high blood pressure during pregnancy and pre-term birth. ... of Obstetrics & Gynaecology, are a major step forward ... blood pressure in pregnancy. The research has been ...
... balance of nature looms prominently in the public mind these ... declining fish stocks are but a few of the issues ... is it? It depends on whom you ask, ... the School of Life Sciences specializing in biology and society. ...
... no group takes credit. The pressure on the U.S. ... the president orders an attack, but it turns out ... Things go downhill from there. , To avoid that ... with extensive nuclear expertise, headed by Stanford physicist Michael ...
Cached Biology News:Herpes virus link to complications in pregnancy 2Managing uncertainty important in ecological balance: ASU researcher 2Group led by Stanford physicist says there's an urgent need for nuclear detectives 2Group led by Stanford physicist says there's an urgent need for nuclear detectives 3
(Date:8/27/2014)... In 2013, Lawrence Livermore National ... Security LLC (LLNS), was awarded more than ... laser system for the European Union's Extreme Light ... the Czech Republic. , When commissioned to its ... " High repetition-rate Advanced Petawatt Laser System " ...
(Date:8/27/2014)... MSC , a healthcare performance improvement company advancing clinical ... Mary Beth Loesch to President and CEO. ... companies for rapid growth and market expansion, Loesch joined ... Development and Healthcare. In that role, she oversaw MSC,s ... corporate strategy and marketing. Previously, Loesch served as Senior ...
(Date:8/27/2014)... 2014 The Global and China Trifluoroacetic ... the current state of the Trifluoroacetic Acid industry in ... overview of the industry, including definitions, applications and industry ... analysis are provided with a focus on history, developments, ... between the international and Chinese situation is also offered. ...
(Date:8/27/2014)... -- Research and Markets  has announced the ... Polymer, Food & Beverage, Personal Care & Pharmaceutical) ... report to their offering. Lactic acid ... carbon atom. The main applications of lactic acid ... personal care product, and pharmaceutical industries. Lactic ...
Breaking Biology Technology:LLNL Synchs up with ELI Beamlines on Timing System 2LLNL Synchs up with ELI Beamlines on Timing System 3MSC names Mary Beth Loesch President and CEO 2Trifluoroacetic Acid Industry (Asia, Europe, North America, ROW) Analysis Report Now Available at ReportsnReports.com 2Global Lactic Acid (Biodegradable Polymer, Food & Beverage, Personal Care & Pharmaceutical) Market - Trends & Forecasts to 2019 2
... United States and Canada and Monetary Damages, ... a medical device company focused on the treatment ... patent infringement lawsuits,against CryoCath Technologies Inc., one in ... Delaware and one in Canada., The lawsuits ...
... Preparing for the NFL, Combine Use SURGEX(TM) as part of ... ... Clubs(TM), BASKING RIDGE, N.J., Jan. 15 ... MBTG), announced today that SURGEX(TM) is,currently being administered as part of ...
... Jan. 15 As drastic cutbacks at ... challenge,blockbuster drug teams. Social networks have entered ... expenditures are being scrutinized for,ROI. Product recalls ... and,responsibilities on drug marketers. Yet new marketing ...
Cached Biology Technology:CryoCor Files Patent Infringement Lawsuits Against CryoCath 2CryoCor Files Patent Infringement Lawsuits Against CryoCath 3Pro Football Prospects Training at TEST Sports Clubs(TM) Use SURGEX(TM) as Part of Their Pre-Combine Training Regime 2Pro Football Prospects Training at TEST Sports Clubs(TM) Use SURGEX(TM) as Part of Their Pre-Combine Training Regime 3Pro Football Prospects Training at TEST Sports Clubs(TM) Use SURGEX(TM) as Part of Their Pre-Combine Training Regime 4Beyond DTC: Pharma Industry Faces New Challenges, Competition, Strategies for Market Dominance 2