Navigation Links
Pathway toward gene silencing described in plants

e siRNAs and also the loci that are modified by the siRNAs," Pikaard said.

Pontes found the other half of the team located within a special nuclear compartment known as the nucleolus, long known to be the production center for ribosomes. "She got a brilliant signal in the nucleolus, a brilliant dot in the same place for each of the proteins," said Pikaard. Using RNA-FISH, Pontes also found that the siRNAs were in that same dot within the nucleolus.

Pontes and Pikaard were able to deduce the order of events by studying mutations of all eight genes that give rise to the proteins, finding out what happens to the different proteins as the different genes are mutated, one by one. For instance, the researchers found the importance of RNA Polymerase IVa (Pol IVa) by looking at a Pol IVa mutant and noting that the rest of the proteins didn't localize properly. In the RNA-dependent RNA polymerase 2 (RDR2) mutant, Pol IVa is unaffected, but the function of all the other proteins downstream is lost, inferring that it came into the act second. The picture that emerged from this logical approach is that Pol IVa gets things started, churning out RNA that then goes to the nucleolus where it is acted on by RDR2, which turns the single-stranded RNA into double-stranded RNA. The Dicer-like 3 protein, DCL3 then chops the RNA into small interfering RNAs (siRNAs). Along comes ARGONAUTE4 (AGO4), which grabs hold of the siRNAs while also binding to NRPD1b, the largest subunit of an alternative form of RNA Polymerase IV, Pol IVb. The AGO4-siRNA-NRPD1b complex is then thought to leave the nucleolus, acquire the second-largest Pol IV subunit, NRPD2, which serves both Pol IVa and Pol IVb, and then seek out the DNA sequences that match the siRNAs. At these sites, the chromatin remodeler DRD1 presumably bulldozes histones and other proteins out of the way to make the DNA accessible for methylation by the de novo cytosine methyltransferase, DRM2.

A paradoxical
'"/>

Source:Washington University in St. Louis


Page: 1 2 3 4

Related biology news :

1. Breast Cancer May Be Uniquely Sensitive To Inhibitors Of PI3K Pathway
2. Measuring Enzymes At End Of Cancer Pathway Predicts Outcome Of Tarceva, Taxol
3. Ariadne Genomics Announces the Release of PathwayStudio?Central, Client-Server Software for Biological Pathway Analysis
4. Unchecked DNA replication drives earliest steps toward cancer
5. Virologists make major step towards understanding the process of HIV infection
6. Moffitt-USF head toward first human trials of anti-cancer drug that targets protein AKT
7. Cats indifference towards sugar explained
8. A step toward the $1,000 personal genome using readily available lab equipment
9. A new step towards an AIDS vaccine
10. New GM mosquito sexing technique is step towards malaria control, report scientists
11. Novel method reveals how menthol discovery could point towards new or improved pain therapies
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Pathway toward gene silencing described plants

(Date:7/28/2014)... have pinpointed a mechanism in part of the brain that ... it to both type 1 and type 2 diabetes. The ... Proceedings of the National Academies of Sciences . , ... a part of the hypothalamus known as the ventromedial nucleus ... glucose levels in the blood," said lead author Sabrina Diano, ...
(Date:7/28/2014)... other forms of dementia, but the research community is ... Washington bioengineers have a designed a peptide structure that ... proteins into a state that,s linked to widespread diseases ... and Lou Gehrig,s disease. The synthetic molecule blocks these ... an abnormally folded form by targeting a toxic intermediate ...
(Date:7/28/2014)... St. Jude Children,s Research Hospital study found that 73 ... doubled their risk of developing metabolic syndrome and related ... The results appear in the current issue of the ... the 1,598 adult survivors of childhood cancer in the ... risk factors like high blood pressure, abdominal obesity, elevated ...
Breaking Biology News(10 mins):Glucose 'control switch' in the brain key to both types of diabetes 2New protein structure could help treat Alzheimer's, related diseases 2New protein structure could help treat Alzheimer's, related diseases 3Unhealthy habits more than double risk of metabolic syndrome in childhood cancer survivors 2Unhealthy habits more than double risk of metabolic syndrome in childhood cancer survivors 3
... Hunting for meat in the African rainforests has halved the ... consequences. The decline in the number of primates causes a ... this leads to a reduction in the numbers of important ... shown in new research from Lund University in Sweden. ...
... 2013 - Elsevier, a world-leading provider of scientific, technical ... announce the launch of Wound Medicine The ... and Applications . Published in print and ... is devoted to the clinical, scientific and health economics ...
... to determine mercury exposure from dental amalgam fillings may ... from fillings, according to University of Michigan researchers. ... vapor into the mouth. But both the amount of ... presents a significant health risk remain controversial. Public ...
Cached Biology News:Hunting for meat impacts on rainforest 2Elsevier announces the launch of a new journal: Wound Medicine 2Measuring mercury: Common test may overestimate exposure from dental amalgam fillings 2Measuring mercury: Common test may overestimate exposure from dental amalgam fillings 3
(Date:7/29/2014)... July 29, 2014   Sequenom, Inc. (NASDAQ: ... provides innovative genetic analysis solutions, and Mayo Medical ... services in the United States , ... patents and applications. "We have great appreciation ... care, and we welcome the opportunity to partner with ...
(Date:7/29/2014)... separated from its properties? On July 29, the prestigious ... the first Cheshire Cat experiment, separating a neutron from ... CA, and Vienna University of Technology. , Chesire Cat"Well! ... Alice in Wonderland, "but a grin without a cat! ... my life!" Alice,s surprise stems from her experience that ...
(Date:7/29/2014)... team of researchers has created a new way ... textures. These surfaces, made by self-assembly of carbon ... including controllable mechanical stiffness and strength, or ... direction. , "We have demonstrated that mechanical forces ... complex three-dimensional microstructures, and that we can independently ...
(Date:7/29/2014)... 29, 2014  Pomerantz LLP is investigating claims on ... the "Company") (NASDAQ: IPXL).  Such investors are advised to ... or 888-476-6529, ext. 237. The investigation ... directors have violated Sections 10(b) and 20(a) of the ... July 29, 2014, Impax Laboratories, Inc. (the "Company") issued ...
Breaking Biology Technology:Sequenom Enters Into License Agreement With Mayo Medical Laboratories 2Sequenom Enters Into License Agreement With Mayo Medical Laboratories 3Sequenom Enters Into License Agreement With Mayo Medical Laboratories 4The quantum Cheshire cat: Scientists separate a particle from its properties 2The quantum Cheshire cat: Scientists separate a particle from its properties 3The quantum Cheshire cat: Scientists separate a particle from its properties 4A new way to make microstructured surfaces 2SHAREHOLDER ALERT: Pomerantz Law Firm Investigates Claims On Behalf of Investors of Impax Laboratories, Inc. - IPXL 2
... Mass., Dec. 15 Repligen Corporation (Nasdaq: RGEN ) ... funding to support the ongoing development of new treatments for ... grant will support the completion of preclinical GLP toxicology testing ... trials. Support from the MDA not only provides important ...
... , , , , , , , ... Board : AEMD) announced today that Dr. Jeff Schorey and Dr. ... Exosome Sciences, Inc. (ESI). Both Dr. Schorey and Dr. Comper are ... of Aethlon Medical, was recently formed to leverage the discovery that ...
... , , PLYMOUTH, Minn., Dec. 15 The Mosaic ... quarter fiscal year 2010 earnings results and financial tables on Tuesday, ... York Stock Exchange. Results will be available on Mosaic,s website ... call with presentation slides on Wednesday, January 6, 2010, to discuss ...
Cached Biology Technology:Repligen Receives Second Research Grant from the Muscular Dystrophy Association to Support Friedreich's Ataxia Preclinical Development Program 2Repligen Receives Second Research Grant from the Muscular Dystrophy Association to Support Friedreich's Ataxia Preclinical Development Program 3Aethlon Medical Appoints Jeff Schorey and Wayne Comper to Exosome Sciences Advisory Board 2Aethlon Medical Appoints Jeff Schorey and Wayne Comper to Exosome Sciences Advisory Board 3Aethlon Medical Appoints Jeff Schorey and Wayne Comper to Exosome Sciences Advisory Board 4Aethlon Medical Appoints Jeff Schorey and Wayne Comper to Exosome Sciences Advisory Board 5Mosaic Announces Second Quarter Fiscal Year 2010 Earnings Release and Conference Call 2
... supplied as 10X concentrated solutions. REact Buffer SetConsists ... the REact buffers and one 250 microl vial ... 1 ml vials of REact 1 Buffer four ... 1 ml vials of REact 3 Buffer and ...
Request Info...
Services include subcloning into an expression vector. Tagged protein will be expressed in small scale for testing yield and solubility and then in 1L culture for large scale affinity purification. E...
...
Biology Products: