Navigation Links
PCRM develops world's first cruelty-free insulin assay

If you're an organization dedicated to humane alternatives to the use of animals in research and you want to conduct research of your own that requires using animals as part of the testing, what do you do? In the case of the Physicians Committee for Responsible Medicine, you invent your own test.

PCRM president Neal Barnard, M.D., announced today that PCRM has developed the world's first cruelty-free insulin assay, a test used to measure insulin levels in individuals with diabetes. The assay, which uses no animals, was developed as part of PCRM's ongoing clinical trials to test the effects of a low-fat, vegan diet on patients with type 2 diabetes.

"We only had two options available to us when we began our diabetes trials," said Dr. Barnard. "One, we could use test kits with insulin antibodies grown in vivo--literally from cells injected into the abdomens of live mice--or we could use kits containing antibodies produced from cells cultured with fetal calf serum. Neither was acceptable to us."

The answer? Develop an in-vitro, or test-tube, procedure using a synthetic replacement for the fetal calf serum used as a culturing medium in millions of medical tests every year.

After months of painstaking detective work, PCRM research analyst Megha Even, M.S., working with BiosPacific, an Emoryville, California, lab, succeeded in culturing cells using an animal component-free, peptide- and protein-free, media supplement as a replacement for calf serum--basically a synthetic formula with cofactors and trace elements that promote cell growth. Then, in collaboration with Linco Research of St. Charles, Missouri, Even successfully incorporated antibodies grown in the medium into a test kit for human insulin.

A report on the new methodology will be published soon in a peer-reviewed journal in conjunction with Linco. Even will present her findings at the "Experimental Biology 2005" scientific conference in San Diego, April 2-6. The new assay ki ts are available commercially from Linco.

"We hope that by making the test readily available and competitively priced, researchers and medical labs will use it," said Barnard. "We have proven that if researchers are willing to make the effort, there are effective, humane alternatives to animal-based assays and other testing procedures--alternatives that could help save the lives of millions of people and animals."

There are an estimated 194 million people worldwide with diabetes. More than 15 million Americans suffer from the disease and resulting complications.


Source:Physicians Committee for Responsible Medicine

Related biology news :

1. Carnegie Mellon develops non-invasive technique to detect transplant rejection at cellular level
2. Engineer ramps up protein production, develops versatile viral spheres
3. Breakthrough: Scientists create worlds tiniest organic particles
4. Results of worlds first gene therapy trial for arthritis show approach safe, feasible
5. Environmental lessons from tsunami as worlds coastal population doubles
6. New plant DNA libraries provides massive boost to worlds plant researchers
7. New book highlights worlds borderless conservation areas
8. Some good news for the worlds poor
9. New study of the worlds smallest elephant
10. The worlds deepest dinosaur finding - 2256 metres below the seabed
11. Scientists a step closer to protecting worlds most important crop
Post Your Comments:

(Date:10/29/2015)... Oct. 29, 2015  Rubicon Genomics, Inc., today ... distribution of its DNA library preparation products, including ... new ThruPLEX Plasma-seq kit. ThruPLEX Plasma-seq has been ... of NGS libraries for liquid biopsies--the analysis of ... prognostic applications in cancer and other conditions. Eurofins ...
(Date:10/27/2015)... 27, 2015 Munich, Germany ... Mapping technology (ASGM) automatically maps data from mobile eye ... , so that they can be quantitatively analyzed ... Munich, Germany , October 28-29, 2015. SMI,s ... from mobile eye tracking videos created with SMI,s ...
(Date:10/23/2015)... DUBLIN , Oct. 23, 2015 Research ... of the "Global Voice Recognition Biometrics Market 2015-2019" ... --> --> The global voice recognition ... during 2014-2019. --> ... 2015-2019, has been prepared based on an in-depth market ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... ... November 24, 2015 , ... Copper is an essential ... bound to proteins, copper is also toxic to cells. With a $1.3 million ... (WPI) will conduct a systematic study of copper in the bacteria Pseudomonas aeruginosa ...
(Date:11/24/2015)... Inc., a worldwide provider of clinical research services headquartered in ... has set a new quarterly earnings record in Q3 of 2015.  ... Q3 of 2014 to Q3 of 2015.   ... the establishment of an Asia-Pacific office to ... and Mexico , with the establishment ...
(Date:11/24/2015)... ... November 24, 2015 , ... The Academy of Model ... Group (SIG), MultiGP, also known as Multirotor Grand Prix, to represent the First–Person View ... years. Many AMA members have embraced this type of racing and several new model ...
(Date:11/24/2015)... Ltd. (OTCQB: TIKRF) today announced that its Annual General Meeting of Shareholders ... Israel time, at the law offices of Goldfarb Seligman ... Floor, Tel Aviv, Israel . ... to the Board of Directors; , election of Liat ... an amendment to certain terms of options granted to our Chief Executive ...
Breaking Biology Technology: